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August-2014 Volume 34 Issue 2

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Article

Osmotic stress decreases aquaporin-4 expression in the human retinal pigment epithelial cell line, ARPE-19

  • Authors:
    • François Willermain
    • Sarah Janssens
    • Tatjana Arsenijevic
    • Isabelle Piens
    • Nargis Bolaky
    • Laure Caspers
    • Jason Perret
    • Christine Delporte
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, CHU Saint-Pierre and Brugmann, Brussels, Belgium, Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, Belgium
  • Pages: 533-538
    |
    Published online on: May 29, 2014
       https://doi.org/10.3892/ijmm.2014.1791
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Abstract

The regulation of water movement is of utmost importance for normal retinal function. Under physiological conditions, water is transported, dependent on the osmotic gradient, through the retinal pigment epithelial cell layer from the subretinal space to the choroid. The osmotic gradient has been found to be modified in eye diseases, thus leading to water accumulation in the subretinal space and the sensory retina, and subsequently contributing to the formation of macular oedema. Understanding the regulation of aquaporin expression is therefore crucial. In this study, we investigated the effects of hyperosmolarity on aquaporin-4 (AQP4) protein expression in the human retinal pigment epithelial cell line, ARPE‑19. AQP4 expression was examined by PCR, western blot analysis and immunofluorescence. Ubiquitinylation was examined by immunoprecipitation. The results revealed that hyperosmotic stress rapidly decreased AQP4 expression in the ARPE-19 cells. The effect remained unmodified by lysosomal or mitogen-activated protein kinase inhibitors, but was reversed by proteasome inhibitors. However, no ubiquitinylation of AQP4 was detected. Our results suggest that hyperosmotic stress markedly reduces AQP4 expression possibly through a proteasome ubiquitinylation-independent pathway. This may represent an adaptation to hyperosmotic stress. The results presented in this study contribute to our understanding of the formation of macular oedema.
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Copy and paste a formatted citation
Spandidos Publications style
Willermain F, Janssens S, Arsenijevic T, Piens I, Bolaky N, Caspers L, Perret J and Delporte C: Osmotic stress decreases aquaporin-4 expression in the human retinal pigment epithelial cell line, ARPE-19. Int J Mol Med 34: 533-538, 2014.
APA
Willermain, F., Janssens, S., Arsenijevic, T., Piens, I., Bolaky, N., Caspers, L. ... Delporte, C. (2014). Osmotic stress decreases aquaporin-4 expression in the human retinal pigment epithelial cell line, ARPE-19. International Journal of Molecular Medicine, 34, 533-538. https://doi.org/10.3892/ijmm.2014.1791
MLA
Willermain, F., Janssens, S., Arsenijevic, T., Piens, I., Bolaky, N., Caspers, L., Perret, J., Delporte, C."Osmotic stress decreases aquaporin-4 expression in the human retinal pigment epithelial cell line, ARPE-19". International Journal of Molecular Medicine 34.2 (2014): 533-538.
Chicago
Willermain, F., Janssens, S., Arsenijevic, T., Piens, I., Bolaky, N., Caspers, L., Perret, J., Delporte, C."Osmotic stress decreases aquaporin-4 expression in the human retinal pigment epithelial cell line, ARPE-19". International Journal of Molecular Medicine 34, no. 2 (2014): 533-538. https://doi.org/10.3892/ijmm.2014.1791
Copy and paste a formatted citation
x
Spandidos Publications style
Willermain F, Janssens S, Arsenijevic T, Piens I, Bolaky N, Caspers L, Perret J and Delporte C: Osmotic stress decreases aquaporin-4 expression in the human retinal pigment epithelial cell line, ARPE-19. Int J Mol Med 34: 533-538, 2014.
APA
Willermain, F., Janssens, S., Arsenijevic, T., Piens, I., Bolaky, N., Caspers, L. ... Delporte, C. (2014). Osmotic stress decreases aquaporin-4 expression in the human retinal pigment epithelial cell line, ARPE-19. International Journal of Molecular Medicine, 34, 533-538. https://doi.org/10.3892/ijmm.2014.1791
MLA
Willermain, F., Janssens, S., Arsenijevic, T., Piens, I., Bolaky, N., Caspers, L., Perret, J., Delporte, C."Osmotic stress decreases aquaporin-4 expression in the human retinal pigment epithelial cell line, ARPE-19". International Journal of Molecular Medicine 34.2 (2014): 533-538.
Chicago
Willermain, F., Janssens, S., Arsenijevic, T., Piens, I., Bolaky, N., Caspers, L., Perret, J., Delporte, C."Osmotic stress decreases aquaporin-4 expression in the human retinal pigment epithelial cell line, ARPE-19". International Journal of Molecular Medicine 34, no. 2 (2014): 533-538. https://doi.org/10.3892/ijmm.2014.1791
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