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International Journal of Molecular Medicine
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August-2014 Volume 34 Issue 2

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Article

α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma

  • Authors:
    • Shinichi Hayashi
    • Tetsuo Mikami
    • Yoshihiro Murai
    • Yasuo Takano
    • Johji Imura
  • View Affiliations / Copyright

    Affiliations: Department of Diagnostic Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan, Department of Pathology, Toho University School of Medicine, Ota, Tokyo 143-8540, Japan, Department of Nursing, Toyama College of Welfare Science, Imizu 939-0341, Japan, Kanagawa Cancer Center Research Institute, Asahi, Yokohama 241-8515, Japan
  • Pages: 483-490
    |
    Published online on: June 4, 2014
       https://doi.org/10.3892/ijmm.2014.1793
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Abstract

In the present study, the newly established mouse monoclonal antibody, Y-49, binding to a specific epitope of α-tubulin, was used to examine immunohistochemical reactivity in 116 patients with non-Hodgkin's lymphoma (NHL). The protein was detected at elevated levels in the nuclei of human proliferating cells by western blot analysis, flow cytometry and immunohistochemical analysis. The relatively weak binding in the cytoplasm was evident in almost all cases. The investigation of the correlation between immuno­histochemical positivity and clinicopathological variables revealed links with the MIB-1 proliferation index and poor survival. Nuclear positivity with Y-49 was more frequent in older-aged patients, those with nodal NHL and in those who harbored the diffuse large B-cell histological subtype, and was strongly associated with high MIB-1 labeling indices (LIs). Survival analysis by the Kaplan-Meier method revealed statistically significant differences between patients with high and low Y-49 LIs (p=0.0181), even in the group with advanced (stage III/IV) disease (p=0.0327). Multivariate analysis revealed that overexpression of α-tubulin is an independent prognostic factor in NHL with a relative risk of 2.786.
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Copy and paste a formatted citation
Spandidos Publications style
Hayashi S, Mikami T, Murai Y, Takano Y and Imura J: α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma. Int J Mol Med 34: 483-490, 2014.
APA
Hayashi, S., Mikami, T., Murai, Y., Takano, Y., & Imura, J. (2014). α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma. International Journal of Molecular Medicine, 34, 483-490. https://doi.org/10.3892/ijmm.2014.1793
MLA
Hayashi, S., Mikami, T., Murai, Y., Takano, Y., Imura, J."α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma". International Journal of Molecular Medicine 34.2 (2014): 483-490.
Chicago
Hayashi, S., Mikami, T., Murai, Y., Takano, Y., Imura, J."α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma". International Journal of Molecular Medicine 34, no. 2 (2014): 483-490. https://doi.org/10.3892/ijmm.2014.1793
Copy and paste a formatted citation
x
Spandidos Publications style
Hayashi S, Mikami T, Murai Y, Takano Y and Imura J: α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma. Int J Mol Med 34: 483-490, 2014.
APA
Hayashi, S., Mikami, T., Murai, Y., Takano, Y., & Imura, J. (2014). α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma. International Journal of Molecular Medicine, 34, 483-490. https://doi.org/10.3892/ijmm.2014.1793
MLA
Hayashi, S., Mikami, T., Murai, Y., Takano, Y., Imura, J."α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma". International Journal of Molecular Medicine 34.2 (2014): 483-490.
Chicago
Hayashi, S., Mikami, T., Murai, Y., Takano, Y., Imura, J."α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma". International Journal of Molecular Medicine 34, no. 2 (2014): 483-490. https://doi.org/10.3892/ijmm.2014.1793
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