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International Journal of Molecular Medicine
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August-2014 Volume 34 Issue 2

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

Brain death induces the alteration of liver protein expression profiles in rabbits

  • Authors:
    • Bing Du
    • Ling Li
    • Zhibiao Zhong
    • Xiaoli Fan
    • Bingbing Qiao
    • Chongxiang He
    • Zhen Fu
    • Yanfeng Wang
    • Qifa Ye
  • View Affiliations / Copyright

    Affiliations: Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, Hubei 430071, P.R. China
  • Pages: 578-584
    |
    Published online on: June 16, 2014
       https://doi.org/10.3892/ijmm.2014.1806
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Abstract

At present, there is no accurate method for evaluating the quality of liver transplant from a brain-dead donor. Proteomics are used to investigate the mechanisms involved in brain death‑induced liver injury and to identify sensitive biomarkers. In the present study, age‑ and gender‑matched rabbits were randomly divided into the brain death and sham groups. The sham served as the control. A brain‑death model was established using an intracranial progressive pressurized method. The differentially expressed proteins extracted from the liver tissues of rabbits that were brain‑dead for 6 h in the two groups were determined by two‑dimensional gel electrophoresis and matrix‑assisted laser desorption ionization time of flight mass spectrometry. Although there was no obvious functional and morphological difference in 2, 4 and 6 h after brain death, results of the proteomics analysis revealed 973±34 and 987±38 protein spots in the control and brain death groups, respectively. Ten proteins exhibited a ≥2‑fold alteration. The downregulated proteins were: aldehyde dehydrogenase, runt‑related transcription factor 1 (RUNX1), inorganic pyrophosphatase, glutamate‑cysteine ligase regulatory subunit and microsomal cytochrome B5. By contrast, the expression of dihydropyrimidinase-related protein 4, peroxiredoxin‑6, 3‑phosphoinositide‑dependent protein kinase‑1, 3-mercaptopyruvate and alcohol dehydrogenase were clearly upregulated. Immunohistochemistry and western blot analysis results revealed that the expression of RUNX1 was gradually increased in a time‑dependent manner in 2, 4, and 6 h after brain death. In conclusion, alteration of the liver protein expression profile induced by brain death indicated the occurrence of complex pathological changes even if no functional or morphological difference was identified. Thus, RUNX1 may be a sensitive predict factor for evaluating the quality of brain death donated liver.
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Copy and paste a formatted citation
Spandidos Publications style
Du B, Li L, Zhong Z, Fan X, Qiao B, He C, Fu Z, Wang Y and Ye Q: Brain death induces the alteration of liver protein expression profiles in rabbits. Int J Mol Med 34: 578-584, 2014.
APA
Du, B., Li, L., Zhong, Z., Fan, X., Qiao, B., He, C. ... Ye, Q. (2014). Brain death induces the alteration of liver protein expression profiles in rabbits. International Journal of Molecular Medicine, 34, 578-584. https://doi.org/10.3892/ijmm.2014.1806
MLA
Du, B., Li, L., Zhong, Z., Fan, X., Qiao, B., He, C., Fu, Z., Wang, Y., Ye, Q."Brain death induces the alteration of liver protein expression profiles in rabbits". International Journal of Molecular Medicine 34.2 (2014): 578-584.
Chicago
Du, B., Li, L., Zhong, Z., Fan, X., Qiao, B., He, C., Fu, Z., Wang, Y., Ye, Q."Brain death induces the alteration of liver protein expression profiles in rabbits". International Journal of Molecular Medicine 34, no. 2 (2014): 578-584. https://doi.org/10.3892/ijmm.2014.1806
Copy and paste a formatted citation
x
Spandidos Publications style
Du B, Li L, Zhong Z, Fan X, Qiao B, He C, Fu Z, Wang Y and Ye Q: Brain death induces the alteration of liver protein expression profiles in rabbits. Int J Mol Med 34: 578-584, 2014.
APA
Du, B., Li, L., Zhong, Z., Fan, X., Qiao, B., He, C. ... Ye, Q. (2014). Brain death induces the alteration of liver protein expression profiles in rabbits. International Journal of Molecular Medicine, 34, 578-584. https://doi.org/10.3892/ijmm.2014.1806
MLA
Du, B., Li, L., Zhong, Z., Fan, X., Qiao, B., He, C., Fu, Z., Wang, Y., Ye, Q."Brain death induces the alteration of liver protein expression profiles in rabbits". International Journal of Molecular Medicine 34.2 (2014): 578-584.
Chicago
Du, B., Li, L., Zhong, Z., Fan, X., Qiao, B., He, C., Fu, Z., Wang, Y., Ye, Q."Brain death induces the alteration of liver protein expression profiles in rabbits". International Journal of Molecular Medicine 34, no. 2 (2014): 578-584. https://doi.org/10.3892/ijmm.2014.1806
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