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Article Open Access

Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries

  • Authors:
    • Teiji Oda
    • Akane Yamaguchi
    • Masao Yokoyama
    • Koji Shimizu
    • Kosaku Toyota
    • Tetsuro Nikai
    • Ken‑Ichi Matsumoto
  • View Affiliations / Copyright

    Affiliations: Division of Thoracic and Cardiovascular Surgery, Department of Surgery, Shimane University Faculty of Medicine, Shimane, Japan, Department of Anesthesiology, Shimane University Faculty of Medicine, Shimane, Japan, Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, Shimane, Japan
    Copyright: © Oda et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 947-956
    |
    Published online on: July 15, 2014
       https://doi.org/10.3892/ijmm.2014.1855
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Abstract

Deep hypothermic circulatory arrest (DHCA) is a protective method against brain ischemia in aortic surgery. However, the possible effects of DHCA on the plasma proteins remain to be determined. In the present study, we used novel high‑throughput technology to compare the plasma proteomes during DHCA (22˚C) with selective cerebral perfusion (SCP, n=7) to those during normothermic cardiopulmonary bypass (CPB, n=7). Three plasma samples per patient were obtained during CPB: T1, prior to cooling; T2, during hypothermia; T3, after rewarming for the DHCA group and three corresponding points for the normothermic group. A proteomic analysis was performed using isobaric tag for relative and absolute quantification (iTRAQ) labeling tandem mass spectrometry to assess quantitative protein changes. In total, the analysis identified 262 proteins. The bioinformatics analysis revealed a significant upregulation of complement activation at T2 in normothermic CPB, which was suppressed in DHCA. These findings were confirmed by the changes of the terminal complement complex (SC5b‑9) levels. At T3, however, the level of SC5b‑9 showed a greater increase in DHCA compared to normothermic CPB, while 48 proteins were significantly downregulated in DHCA. The results demonstrated that DHCA and rewarming potentially exert a significant effect on the plasma proteome in patients undergoing aortic surgery.
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Copy and paste a formatted citation
Spandidos Publications style
Oda T, Yamaguchi A, Yokoyama M, Shimizu K, Toyota K, Nikai T and Matsumoto KI: Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries. Int J Mol Med 34: 947-956, 2014.
APA
Oda, T., Yamaguchi, A., Yokoyama, M., Shimizu, K., Toyota, K., Nikai, T., & Matsumoto, K. (2014). Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries. International Journal of Molecular Medicine, 34, 947-956. https://doi.org/10.3892/ijmm.2014.1855
MLA
Oda, T., Yamaguchi, A., Yokoyama, M., Shimizu, K., Toyota, K., Nikai, T., Matsumoto, K."Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries". International Journal of Molecular Medicine 34.4 (2014): 947-956.
Chicago
Oda, T., Yamaguchi, A., Yokoyama, M., Shimizu, K., Toyota, K., Nikai, T., Matsumoto, K."Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries". International Journal of Molecular Medicine 34, no. 4 (2014): 947-956. https://doi.org/10.3892/ijmm.2014.1855
Copy and paste a formatted citation
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Spandidos Publications style
Oda T, Yamaguchi A, Yokoyama M, Shimizu K, Toyota K, Nikai T and Matsumoto KI: Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries. Int J Mol Med 34: 947-956, 2014.
APA
Oda, T., Yamaguchi, A., Yokoyama, M., Shimizu, K., Toyota, K., Nikai, T., & Matsumoto, K. (2014). Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries. International Journal of Molecular Medicine, 34, 947-956. https://doi.org/10.3892/ijmm.2014.1855
MLA
Oda, T., Yamaguchi, A., Yokoyama, M., Shimizu, K., Toyota, K., Nikai, T., Matsumoto, K."Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries". International Journal of Molecular Medicine 34.4 (2014): 947-956.
Chicago
Oda, T., Yamaguchi, A., Yokoyama, M., Shimizu, K., Toyota, K., Nikai, T., Matsumoto, K."Plasma proteomic changes during hypothermic and normothermic cardiopulmonary bypass in aortic surgeries". International Journal of Molecular Medicine 34, no. 4 (2014): 947-956. https://doi.org/10.3892/ijmm.2014.1855
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