Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
October 2014 Volume 34 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October 2014 Volume 34 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study

  • Authors:
    • Wen Zhang
    • Xiaolei Zhang
    • Junqi Ling
    • Wei Liu
    • Xinchun  Zhang
    • Jinglei Ma
    • Jianmao Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology and Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510080, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1004-1012
    |
    Published online on: July 24, 2014
       https://doi.org/10.3892/ijmm.2014.1862
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Stem cells from the apical papilla (SCAP) have odontogenic potential, which plays a pivotal role in the root dentin development of permanent teeth. Human bone morphogenetic protein 2 (BMP2) is a well-known gene that participates in regulating the odontogenic differentiation of dental tissue‑derived stem cells. However, little is known regarding the effects of the BMP2 gene on the proliferation and odontogenic differentiation of SCAP. This study aimed to evaluate the odontogenic differentiation potential of lentiviral‑mediated BMP2 gene‑transfected human SCAP (SCAP/BMP2) in vitro. SCAP were isolated by enzymatic dissociation of human teeth apical papillae. The multipotential of SCAP was verified by their osteogenic and adipogenic differentiation characteristics. The phenotype of SCAP was evaluated by flow cytometry (FCM). The proliferation status of the blank vector‑transfected SCAP (SCAP/Vector) and SCAP/BMP2 was analyzed by a cell counting kit-8 (CCK‑8). Odontogenic genes, including alkaline phosphatase (ALP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP1) of the two groups of cells were evaluated by quantitative polymerase chain reaction (qPCR). ALP staining and alizarin red (AR) staining of the cells was performed on the 16th day after transfection. In vitro results of CCK-8, qPCR, ALP and AR staining demonstrated that: ⅰ) SCAP/BMP2 had a comparable proliferation rate to SCAP/Vector; ⅱ) SCAP/BMP2 presented significantly better potential to differentiate into odontoblasts compared to SCAP/Vector by upregulating ALP, OCN, DSPP and DMP1 genes; ⅲ) more ALP granules and mineralized deposits were formed by SCAP/BMP2 as compared to SCAP/Vector. The results suggested that lentiviral-mediated BMP2 gene transfection enhances the odontogenic differentiation capacity of human SCAP in vitro.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Sonoyama W, Liu Y, Fang D, et al: Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS One. 1:e792006. View Article : Google Scholar : PubMed/NCBI

2 

Wu J, Huang GT, He W, et al: Basic fibroblast growth factor enhances stemness of human stem cells from the apical papilla. J Endod. 38:614–622. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Bakopoulou A, Leyhausen G, Volk J, et al: Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP). Arch Oral Biol. 56:709–721. 2011. View Article : Google Scholar

4 

Bakopoulou A, Leyhausen G, Volk J, Koidis P and Geurtsen W: Comparative characterization of (neg)/CD146(pos) and STRO-1 (neg)/CD146(pos) apical papilla stem cells enriched with flow cytometry. Arch Oral Biol. 58:1556–1568. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Sonoyama W, Liu Y, Yamaza T, et al: Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study. J Endod. 34:166–171. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Huang GT, Sonoyama W, Liu Y, Liu H, Wang S and Shi S: The hidden treasure in apical papilla: the potential role in pulp/dentin regeneration and bioroot engineering. J Endod. 34:645–651. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Huang GT, Gronthos S and Shi S: Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 88:792–806. 2009.PubMed/NCBI

8 

Yang W, Harris MA, Cui Y, Mishina Y, Harris SE and Gluhak-Heinrich J: Bmp2 is required for odontoblast differentiation and pulp vasculogenesis. J Dent Res. 91:58–64. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Wang Y, Li L, Zheng Y, et al: BMP activity is required for tooth development from the lamina to bud stage. J Dent Res. 91:690–695. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Rakian A, Yang WC, Gluhak-Heinrich J, et al: Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium. Int J Oral Sci. 5:75–84. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Saito T, Ogawa M, Hata Y and Bessho K: Acceleration effect of human recombinant bone morphogenetic protein-2 on differentiation of human pulp cells into odontoblasts. J Endod. 30:205–208. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Iohara K, Nakashima M, Ito M, Ishikawa M, Nakasima A and Akamine A: Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2. J Dent Res. 83:590–595. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Yang X, van der Kraan PM, van den Dolder J, et al: STRO-1 selected rat dental pulp stem cells transfected with adenoviral-mediated human bone morphogenetic protein 2 gene show enhanced odontogenic differentiation. Tissue Eng. 13:2803–2812. 2007. View Article : Google Scholar

14 

Yang X, Walboomers XF, van den Dolder J, et al: Non-viral bone morphogenetic protein 2 transfection of rat dental pulp stem cells using calcium phosphate nanoparticles as carriers. Tissue Eng Part A. 14:71–81. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Yang X, van der Kraan PM, Bian Z, Fan M, Walboomers XF and Jansen JA: Mineralized tissue formation by BMP2-transfected pulp stem cells. J Dent Res. 88:1020–1025. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Yang X, Walboomers XF, van den Beucken JJ, Bian Z, Fan M and Jansen JA: Hard tissue formation of STRO-1-selected rat dental pulp stem cells in vivo. Tissue Eng Part A. 15:367–375. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Lahmy R, Soleimani M, Sanati MH, Behmanesh M, Kouhkan F and Mobarra N: Pancreatic islet differentiation of human embryonic stem cells by microRNA overexpression. J Tissue Eng Regen Med. 30: View Article : Google Scholar : 2013.PubMed/NCBI

18 

Hwang SY, Foley J, Numaga-Tomita T, Petranka JG, Bird GS and Putney JW Jr: Deletion of Orai1 alters expression of multiple genes during osteoclast and osteoblast maturation. Cell Calcium. 52:488–500. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Wei X, Wu L, Ling J, et al: Differentially expressed protein profile of human dental pulp cells in the early process of odontoblast-like differentiation in vitro. J Endod. 34:1077–1084. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Langenbach F and Handschel J: Effects of dexamethasone, ascorbic acid and β-glycerophosphate on the osteogenic differentiation of stem cells in vitro. Stem Cell Res Ther. 4:1172013.

21 

Hitomi K, Torii Y and Tsukagoshi N: Increase in the activity of alkaline phosphatase by L-ascorbic acid 2-phosphate in a human osteoblast cell line, HuO-3N1. J Nutr Sci Vitaminol (Tokyo). 38:535–544. 1992. View Article : Google Scholar : PubMed/NCBI

22 

Shima N, Kimoto M, Yamaguchi M and Yamagami S: Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate. Invest Ophthalmol Vis Sci. 52:8711–8717. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Choi KS, Ahn SY, Kim TS, et al: Characterization and biodistribution of human mesenchymal stem cells transduced with lentiviral-mediated BMP2. Arch Pharm Res. 34:599–606. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Kutner RH, Zhang XY and Reiser J: Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors. Nat Protoc. 4:495–505. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Reiser J: Production and concentration of pseudotyped HIV-1-based gene transfer vectors. Gene Ther. 7:910–913. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Li R, Peng L, Ren L, Tan H and Ye L: Hepatocyte growth factor exerts promoting functions on murine dental papilla cells. J Endod. 35:382–388. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Choi MH, Noh WC, Park JW, Lee JM and Suh JY: Gene expression pattern during osteogenic differentiation of human periodontal ligament cells in vitro. J Periodontal Implant Sci. 41:167–175. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Yu J, Deng Z, Shi J, et al: Differentiation of dental pulp stem cells into regular-shaped dentin-pulp complex induced by tooth germ cell conditioned medium. Tissue Eng. 12:3097–3105. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Wang C, Ren L, Peng L, Xu P, Dong G and Ye L: Effect of Wnt6 on human dental papilla cells in vitro. J Endod. 36:238–243. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Chen K, Xiong H, Huang Y and Liu C: Comparative analysis of in vitro periodontal characteristics of stem cells from apical papilla (SCAP) and periodontal ligament stem cells (PDLSCs). Arch Oral Biol. 58:997–1006. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Yue J, Wu B, Gao J, et al: DMP1 is a target of let-7 in dental pulp cells. Int J Mol Med. 30:295–301. 2012.PubMed/NCBI

32 

Feng JQ, Huang H, Lu Y, et al: The Dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. J Dent Res. 82:776–780. 2003. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang W, Zhang X, Ling J, Liu W, Zhang X, Ma J and Zheng J: Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study. Int J Mol Med 34: 1004-1012, 2014.
APA
Zhang, W., Zhang, X., Ling, J., Liu, W., Zhang, X., Ma, J., & Zheng, J. (2014). Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study. International Journal of Molecular Medicine, 34, 1004-1012. https://doi.org/10.3892/ijmm.2014.1862
MLA
Zhang, W., Zhang, X., Ling, J., Liu, W., Zhang, X., Ma, J., Zheng, J."Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study". International Journal of Molecular Medicine 34.4 (2014): 1004-1012.
Chicago
Zhang, W., Zhang, X., Ling, J., Liu, W., Zhang, X., Ma, J., Zheng, J."Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study". International Journal of Molecular Medicine 34, no. 4 (2014): 1004-1012. https://doi.org/10.3892/ijmm.2014.1862
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang W, Zhang X, Ling J, Liu W, Zhang X, Ma J and Zheng J: Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study. Int J Mol Med 34: 1004-1012, 2014.
APA
Zhang, W., Zhang, X., Ling, J., Liu, W., Zhang, X., Ma, J., & Zheng, J. (2014). Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study. International Journal of Molecular Medicine, 34, 1004-1012. https://doi.org/10.3892/ijmm.2014.1862
MLA
Zhang, W., Zhang, X., Ling, J., Liu, W., Zhang, X., Ma, J., Zheng, J."Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study". International Journal of Molecular Medicine 34.4 (2014): 1004-1012.
Chicago
Zhang, W., Zhang, X., Ling, J., Liu, W., Zhang, X., Ma, J., Zheng, J."Proliferation and odontogenic differentiation of BMP2 gene‑transfected stem cells from human tooth apical papilla: An in vitro study". International Journal of Molecular Medicine 34, no. 4 (2014): 1004-1012. https://doi.org/10.3892/ijmm.2014.1862
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team