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International Journal of Molecular Medicine
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Article

Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells

  • Authors:
    • Kumiko Tanabe
    • Rie Matsushima-Nishiwaki
    • Osamu Kozawa
    • Hiroki Iida
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology and Pain Medicine, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan
  • Pages: 1032-1038
    |
    Published online on: July 24, 2014
       https://doi.org/10.3892/ijmm.2014.1863
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Abstract

Dexmedetomidine, an α2-adrenoceptor agonist, is used as a sedative medication for criticalyl ill patients and is known to exert neuroprotective effects by direct action on neurons and indirect action on neurons through astrocytes. Interleukin (IL)-6 plays a key role in neuroinflammation, which accompanies infection, traumatic brain injury, ischemia, neurodegenerative disorders, as both a pro-inflammatory cytokine and an anti-inflammatory cytokine. Dexmedetomidine suppresses immune function. However, the effects of dexmedetomidine on cytokine synthesis in the central nervous system (CNS) remain elusive. We previously reported that IL-1β stimulates IL-6 synthesis in the rat C6 glioma cell line through the phosphorylation of p38 mitogen-activated protein (MAP) kinase, stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) and IκB. In the present study, we investigated the effects of dexmedetomidine on the IL-1β-induced IL-6 synthesis in C6 cells. Dexmedetomidine inhibited the IL-1β-stimulated IL-6 release and mRNA expression in C6 cells. 8-Bromo-adenosine-3',5'-cyclic monophosphate, but not 8-bromo-guanosine 3',5'-cyclic monophosphate, significantly enhanced the IL-1β-induced IL-6 release and mRNA expression. However, dexmedetomidine failed to affect cAMP accumulation in the cells treated with IL-1β or forskolin, an activator of adenylyl cyclase. Yohimbine, an α2-adrenoceptor antagonist, did not reverse the suppressive effects of dexmedetomidine on the IL-1β-induced IL-6 release. Dexmedetomidine did not affect the IL-1β-induced phosphorylation of p38 MAP kinase, SAPK/JNK, IκB, nuclear factor (NF)-κB or c-Jun. Our findings strongly suggest that dexmedetomidine inhibits the IL-1β-induced IL-6 synthesis independently of the adenylyl cyclase-cAMP pathway through α2-adrenoceptors in C6 glioma cells. It is possible that dexmedetomidine may affect the immune system in the CNS by regulating the production of IL-6.
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Copy and paste a formatted citation
Spandidos Publications style
Tanabe K, Matsushima-Nishiwaki R, Kozawa O and Iida H: Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells. Int J Mol Med 34: 1032-1038, 2014.
APA
Tanabe, K., Matsushima-Nishiwaki, R., Kozawa, O., & Iida, H. (2014). Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells. International Journal of Molecular Medicine, 34, 1032-1038. https://doi.org/10.3892/ijmm.2014.1863
MLA
Tanabe, K., Matsushima-Nishiwaki, R., Kozawa, O., Iida, H."Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells". International Journal of Molecular Medicine 34.4 (2014): 1032-1038.
Chicago
Tanabe, K., Matsushima-Nishiwaki, R., Kozawa, O., Iida, H."Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells". International Journal of Molecular Medicine 34, no. 4 (2014): 1032-1038. https://doi.org/10.3892/ijmm.2014.1863
Copy and paste a formatted citation
x
Spandidos Publications style
Tanabe K, Matsushima-Nishiwaki R, Kozawa O and Iida H: Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells. Int J Mol Med 34: 1032-1038, 2014.
APA
Tanabe, K., Matsushima-Nishiwaki, R., Kozawa, O., & Iida, H. (2014). Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells. International Journal of Molecular Medicine, 34, 1032-1038. https://doi.org/10.3892/ijmm.2014.1863
MLA
Tanabe, K., Matsushima-Nishiwaki, R., Kozawa, O., Iida, H."Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells". International Journal of Molecular Medicine 34.4 (2014): 1032-1038.
Chicago
Tanabe, K., Matsushima-Nishiwaki, R., Kozawa, O., Iida, H."Dexmedetomidine suppresses interleukin-1β-induced interleukin-6 synthesis in rat glial cells". International Journal of Molecular Medicine 34, no. 4 (2014): 1032-1038. https://doi.org/10.3892/ijmm.2014.1863
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