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Achyranthes bidentata polysaccharides activate the Wnt/β-catenin signaling pathway to promote chondrocyte proliferation

  • Authors:
    • Xiaping Weng
    • Pingdong Lin
    • Fayuan Liu
    • Jiashou Chen
    • Huiting Li
    • Lichan Huang
    • Chunsong Zhen
    • Huifeng Xu
    • Xianxiang Liu
    • Hongzhi Ye
    • Xihai Li

  • View Affiliations

  • Published online on: July 29, 2014     https://doi.org/10.3892/ijmm.2014.1869
  • Pages: 1045-1050
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Abstract

Achyranthes bidentata polysaccharides (ABPS) are the active components of Radix Achyranthis Bidentatae (AB), which has been extensively used in Traditional Chinese medicine (TCM) in the treatment of osteoarthritis (OA). Our previous study provided evidence that ABPS regulated the G1/S transition to promote chondrocyte proliferation. However, the precise mechanisms involved remain to be elucidated. In the present study, we aimed to investigate the effects of ABPS on the Wnt/β‑catenin signaling pathway in chondrocytes. Chondrocytes, obtained from the knee cartilage of Sprague-Dawley rats, were identified by type II collagen immunohistochemistry. ABPS upregulated the expression of Wnt-4, Frizzled-2, β-catenin and cyclin D1, and downregulated the expression of glycogen synthase kinase 3β (GSK-3β), as shown by reverse transcription PCR (RT-PCR) and western blot analysis. Using immunofluorescence, we also found that ABPS induced β-catenin nuclear translocation. Importantly, the expression of β-catenin and cyclin D1 was partly inhibited by Dickkopf-1 (DKK-1), an inhibitor of the Wnt/β-catenin signaling pathway. In addition, we found that ABPS increased the expression of type II collagen in chondrocytes. These results suggest that ABPS promote chondrocyte proliferation by activating the Wnt/β-catenin signaling pathway.
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Journal Cover

October 2014
Volume 34 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Journal Metrics
  • Impact Factor: 5.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
  • SCImago Journal Rank (SJR): 0.922
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Spandidos Publications style
Weng X, Lin P, Liu F, Chen J, Li H, Huang L, Zhen C, Xu H, Liu X, Ye H, Ye H, et al: Achyranthes bidentata polysaccharides activate the Wnt/β-catenin signaling pathway to promote chondrocyte proliferation. Int J Mol Med 34: 1045-1050, 2014
APA
Weng, X., Lin, P., Liu, F., Chen, J., Li, H., Huang, L. ... Li, X. (2014). Achyranthes bidentata polysaccharides activate the Wnt/β-catenin signaling pathway to promote chondrocyte proliferation. International Journal of Molecular Medicine, 34, 1045-1050. https://doi.org/10.3892/ijmm.2014.1869
MLA
Weng, X., Lin, P., Liu, F., Chen, J., Li, H., Huang, L., Zhen, C., Xu, H., Liu, X., Ye, H., Li, X."Achyranthes bidentata polysaccharides activate the Wnt/β-catenin signaling pathway to promote chondrocyte proliferation". International Journal of Molecular Medicine 34.4 (2014): 1045-1050.
Chicago
Weng, X., Lin, P., Liu, F., Chen, J., Li, H., Huang, L., Zhen, C., Xu, H., Liu, X., Ye, H., Li, X."Achyranthes bidentata polysaccharides activate the Wnt/β-catenin signaling pathway to promote chondrocyte proliferation". International Journal of Molecular Medicine 34, no. 4 (2014): 1045-1050. https://doi.org/10.3892/ijmm.2014.1869