FPDHP, a novel anticancer agent, induces cell detachment and caspase-dependent apoptosis in Caki cells

Park,Jun  Soo; Kim,Wan  Tae; Kim,Shin; Kwon,Taeg  Kyu; Jang,Byeong-Churl; Lee,Eung-Seok; Park,Jong  Wook

doi:10.3892/ijmm.2014.1875

October 2014 Volume 34 Issue 4

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October 2014 Volume 34 Issue 4

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Article

FPDHP, a novel anticancer agent, induces cell detachment and caspase-dependent apoptosis in Caki cells

Authors:
- Jun Soo Park
- Wan Tae Kim
- Shin Kim
- Taeg Kyu Kwon
- Byeong-Churl Jang
- Eung-Seok Lee
- Jong Wook Park
View Affiliations / Copyright

Affiliations: Chronic Disease Research Center, Keimyung University School of Medicine, Daegu 704-701, Republic of Korea, College of Pharmacy, Yeungnam University, Kyongsan 712-749, Republic of Korea
Pages: 1051-1056
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Published online on: August 4, 2014

https://doi.org/10.3892/ijmm.2014.1875
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Abstract

The inhibition of topoisomerase can suppress the growth of cancer cells and induce apoptosis. The aim of this study was to evaluate the anticancer effects and mechanisms of action of a novel topoisomerase inhibitor, 4-(furan-2-yl)-2-(pyridin-2-yl)-5,6-dihydro-1,10-phenanthroline (FPDHP). FPDHP suppressed the growth of Caki, A549, HT29 and MDA-MB-231 cells, and induced caspase-dependent apoptosis in the Caki cells. In particular, FPDHP also induced caspase-dependent apoptosis and the downregulation of the protein expression levels of cellular FLICE-like inhibitory protein (cFLIP) and the phosphorylation of Akt in Caki cells. Notably, the overexpression of cFLIP, but not that of Akt, in part, blocked the FPDHP-mediated apoptosis in Caki cells. In addition, FPDHP was further shown to induce the caspase-independent detachment of Caki cells from the culture dish; higher populations of apoptotic cells were observed in the detached cells than in the attached cells. To the best of our knoweledge, these results collectively demonstrate for the first time that FPDHP has a killing effect on Caki cells, which is mediated through both caspase-dependent apoptosis and caspase-independent cell detachment.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

FPDHP, a novel anticancer agent, induces cell detachment and caspase-dependent apoptosis in Caki cells

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Abstract

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