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Article

Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation

  • Authors:
    • Michaël Beck
    • Charlotte Rombouts
    • Marjan Moreels
    • An Aerts
    • Roel Quintens
    • Kevin Tabury
    • Arlette Michaux
    • Ann Janssen
    • Mieke Neefs
    • Eric Ernst
    • Birger Dieriks
    • Ryonfa Lee
    • Winnok H. De Vos
    • Charles Lambert
    • Patrick Van Oostveldt
    • Sarah Baatout
  • View Affiliations / Copyright

    Affiliations: Laboratory of Radiobiology, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK‑CEN), Mol, Belgium, Laboratory of Connective Tissues Biology, GIGA-Cancer, University of Liège, Liège, Belgium, Department of Molecular Biotechnology, Ghent University, Ghent, Belgium, Biophysics Department, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany
  • Pages: 1124-1132
    |
    Published online on: August 11, 2014
       https://doi.org/10.3892/ijmm.2014.1893
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Abstract

Ionizing radiation can elicit harmful effects on the cardiovascular system at high doses. Endothelial cells are critical targets in radiation-induced cardiovascular damage. Astronauts performing a long-term deep space mission are exposed to consistently higher fluences of ionizing radiation that may accumulate to reach high effective doses. In addition, cosmic radiation contains high linear energy transfer (LET) radiation that is known to produce high values of relative biological effectiveness (RBE). The aim of this study was to broaden the understanding of the molecular response to high LET radiation by investigating the changes in gene expression in endothelial cells. For this purpose, a human endothelial cell line (EA.hy926) was irradiated with accelerated nickel ions (Ni) (LET, 183 keV/µm) at doses of 0.5, 2 and 5 Gy. DNA damage was measured 2 and 24 h following irradiation by γ-H2AX foci detection by fluorescence microscopy and gene expression changes were measured by microarrays at 8 and 24 h following irradiation. We found that exposure to accelerated nickel particles induced a persistent DNA damage response up to 24 h after treatment. This was accompanied by a downregulation in the expression of a multitude of genes involved in the regulation of the cell cycle and an upregulation in the expression of genes involved in cell cycle checkpoints. In addition, genes involved in DNA damage response, oxidative stress, apoptosis and cell-cell signaling (cytokines) were found to be upregulated. An in silico analysis of the involved genes suggested that the transcription factors, E2F and nuclear factor (NF)-κB, may be involved in these cellular responses.
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1 

Schultz-Hector S and Trott KR: Radiation-induced cardiovascular diseases: is the epidemiologic evidence compatible with the radiobiologic data? Int J Radiat Oncol Biol Phys. 67:10–18. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Blaber E, Marçal H and Burns BP: Bioastronautics: the influence of microgravity on astronaut health. Astrobiology. 10:463–473. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Brinckmann E: Biology in Space and Life on Earth: Effects of Spaceflight on Biological Systems. WILEY-VCH Verlag GmbH & Co. KGaA; Weinheim: 2007, View Article : Google Scholar

4 

Rong Y and Welsh J: Basics of particle therapy II biologic and dosimetric aspects of clinical hadron therapy. Am J Clin Oncol. 33:646–649. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Blakely EA and Chang PY: Biology of charged particles. Cancer J. 15:271–284. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Halle M, Hall P and Tornvall P: Cardiovascular disease associated with radiotherapy: Activation of nuclear factor kappa-B. J Intern Med. 269:469–477. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Hildebrandt G: Non-cancer diseases and non-targeted effects. Mutat Res. 687:73–77. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Rödel F, Frey B, Capalbo G, et al: Discontinuous induction of x-linked inhibitor of apoptosis in ea. Hy926 endothelial cells is linked to NF-κB activation and mediates the anti-inflammatory properties of low-dose ionising-radiation. Radiother Oncol. 97:346–351. 2011.PubMed/NCBI

9 

Rödel F, Hofmann D, Auer J, et al: The anti-inflammatory effect of low-dose radiation therapy involves a diminished CCL20 chemokine expression and granulocyte/endothelial cell adhesion. Strahlenther Onkol. 184:41–47. 2008.

10 

Rödel F, Keilholz L, Herrmann M, Sauer R and Hildebrandt G: Radiobiological mechanisms in inflammatory diseases of low-dose radiation therapy. Int J Radiat Biol. 83:357–366. 2007.PubMed/NCBI

11 

Rödel F, Schaller U, Schultze-Mosgau S, et al: The induction of TGF-beta(1) and NF-kappaB parallels a biphasic time course of leukocyte/endothelial cell adhesion following low-dose X-irradiation. Strahlenther Onkol. 180:194–200. 2004.PubMed/NCBI

12 

Ando K, Ishibashi T, Ohkawara H, et al: Crucial role of membrane type 1 matrix metalloproteinase (MT1-MMP) in Rhoa/Rac1-dependent signaling pathways in thrombin-stimulated endothelial cells. J Atheroscler Thromb. 18:762–773. 2011. View Article : Google Scholar

13 

Kantak SS, Diglio CA and Onoda JM: Low dose radiation-induced endothelial cell retraction. Int J Radiat Biol. 64:319–328. 1993. View Article : Google Scholar : PubMed/NCBI

14 

Onoda JM, Kantak SS and Diglio CA: Radiation induced endothelial cell retraction in vitro: correlation with acute pulmonary edema. Pathol Oncol Res. 5:49–55. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Gabrys D, Greco O, Patel G, Prise KM, Tozer GM and Kanthou C: Radiation effects on the cytoskeleton of endothelial cells and endothelial monolayer permeability. Int J Radiat Oncol Biol Phys. 69:1553–1562. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Pluder F, Barjaktarovic Z, Azimzadeh O, et al: Low-dose irradiation causes rapid alterations to the proteome of the human endothelial cell line EA. hy926. Radiat Environ Biophys. 50:155–166. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Grabham P, Hu B, Sharma P and Geard C: Effects of ionizing radiation on three-dimensional human vessel models: differential effects according to radiation quality and cellular development. Radiat Res. 175:21–28. 2011. View Article : Google Scholar

18 

Takahashi Y, Teshima T, Kawaguchi N, Hamada Y, Mori S, Madachi A, Ikeda S, Mizuno H, Ogata T, Nojima K, Furusawa Y and Matsuura N: Heavy ion irradiation inhibits in vitro angiogenesis even at sublethal dose. Cancer Res. 63:4253–4257. 2003.PubMed/NCBI

19 

Kiyohara H, Ishizaki Y, Suzuki Y, Katoh H, Hamada N, Ohno T, Takahashi T, Kobayashi Y and Nakano T: Radiation-induced ICAM-1 expression via TGF-β1 pathway on human umbilical vein endothelial cells; comparison between X-ray and carbon-ion beam irradiation. J Radiat Res. 52:287–292. 2011.PubMed/NCBI

20 

Haberer T, Becher W, Schardt D and Kraft G: Magnetic scanning system for heavy ion therapy. Nucl Instrum Methods Phys Res A. 330:296–305. 1993. View Article : Google Scholar

21 

De Vos WH, Van Neste L, Dieriks B, Joss GH and Van Oostveldt P: High content image cytometry in the context of subnuclear organization. Cytometry A. 77:64–75. 2010.PubMed/NCBI

22 

Zambelli F, Pesole G and Pavesi G: Pscan: finding over-represented transcription factor binding site motifs in sequences from co-regulated or co-expressed genes. Nucleic Acids Res. 37:W247–W252. 2009. View Article : Google Scholar

23 

Fokas E, Kraft G, An H and Engenhart-Cabillic R: Ion beam radiobiology and cancer: time to update ourselves. Biochim Biophys Acta. 1796:216–229. 2009.PubMed/NCBI

24 

Held KD: Effects of low fluences of radiations found in space on cellular systems. Int J Radiat Biol. 85:379–390. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Costes SV, Boissière A, Ravani S, Romano R, Parvin B and Barcellos-Hoff MH: Imaging features that discriminate between foci induced by high- and low-let radiation in human fibroblasts. Radiat Res. 165:505–515. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Blakely EA and Kronenberg A: Heavy-ion radiobiology: new approaches to delineate mechanisms underlying enhanced biological effectiveness. Radiat Res. 150:S126–S145. 1998. View Article : Google Scholar

27 

Chappell LJ, Whalen MK, Gurai S, Ponomarev A, Cucinotta FA and Pluth JM: Analysis of flow cytometry DNA damage response protein activation kinetics after exposure to x rays and high-energy iron nuclei. Radiat Res. 174:691–702. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Dyson N: The regulation of E2F by pRB-family proteins. Genes Dev. 12:2245–2262. 1998. View Article : Google Scholar : PubMed/NCBI

29 

Biswas AK and Johnson DG: Transcriptional and nontranscriptional functions of E2F1 in response to DNA damage. Cancer Res. 72:13–17. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Costa A and Onesti S: The MCM complex: (just) a replicative helicase? Biochem Soc Trans. 36:136–140. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Forsburg SL: The MCM helicase: Linking checkpoints to the replication fork. Biochem Soc Trans. 36:114–119. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Chuang CH, Yang D, Bai G, Freeland A, Pruitt SC and Schimenti JC: Post-transcriptional homeostasis and regulation of MCM2-7 in mammalian cells. Nucleic Acids Res. 40:4914–4924. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Dejana E, Orsenigo F, Molendini C, Baluk P and McDonald DM: Organization and signaling of endothelial cell-to-cell junctions in various regions of the blood and lymphatic vascular trees. Cell Tissue Res. 335:17–25. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Prasain N and Stevens T: The actin cytoskeleton in endothelial cell phenotypes. Microvasc Res. 77:53–63. 2009. View Article : Google Scholar

35 

Bogatcheva NV and Verin AD: The role of cytoskeleton in the regulation of vascular endothelial barrier function. Microvasc Res. 76:202–207. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Oeckinghaus A and Ghosh S: The NF-kappaB family of transcription factors and its regulation. Cold Spring Harb Perspect Biol. 1:a0000342009. View Article : Google Scholar : PubMed/NCBI

37 

Karin M: NF-kappaB as a critical link between inflammation and cancer. Cold Spring Harb Perspect Biol. 1:a0001412009. View Article : Google Scholar : PubMed/NCBI

38 

Dieriks B, De Vos WH, Derradji H, Baatout S and Van Oostveldt P: Medium-mediated DNA repair response after ionizing radiation is correlated with the increase of specific cytokines in human fibroblasts. Mutat Res. 687:40–48. 2010. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Beck M, Rombouts C, Moreels M, Aerts A, Quintens R, Tabury K, Michaux A, Janssen A, Neefs M, Ernst E, Ernst E, et al: Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation. Int J Mol Med 34: 1124-1132, 2014.
APA
Beck, M., Rombouts, C., Moreels, M., Aerts, A., Quintens, R., Tabury, K. ... Baatout, S. (2014). Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation. International Journal of Molecular Medicine, 34, 1124-1132. https://doi.org/10.3892/ijmm.2014.1893
MLA
Beck, M., Rombouts, C., Moreels, M., Aerts, A., Quintens, R., Tabury, K., Michaux, A., Janssen, A., Neefs, M., Ernst, E., Dieriks, B., Lee, R., De Vos, W. H., Lambert, C., Van Oostveldt, P., Baatout, S."Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation". International Journal of Molecular Medicine 34.4 (2014): 1124-1132.
Chicago
Beck, M., Rombouts, C., Moreels, M., Aerts, A., Quintens, R., Tabury, K., Michaux, A., Janssen, A., Neefs, M., Ernst, E., Dieriks, B., Lee, R., De Vos, W. H., Lambert, C., Van Oostveldt, P., Baatout, S."Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation". International Journal of Molecular Medicine 34, no. 4 (2014): 1124-1132. https://doi.org/10.3892/ijmm.2014.1893
Copy and paste a formatted citation
x
Spandidos Publications style
Beck M, Rombouts C, Moreels M, Aerts A, Quintens R, Tabury K, Michaux A, Janssen A, Neefs M, Ernst E, Ernst E, et al: Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation. Int J Mol Med 34: 1124-1132, 2014.
APA
Beck, M., Rombouts, C., Moreels, M., Aerts, A., Quintens, R., Tabury, K. ... Baatout, S. (2014). Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation. International Journal of Molecular Medicine, 34, 1124-1132. https://doi.org/10.3892/ijmm.2014.1893
MLA
Beck, M., Rombouts, C., Moreels, M., Aerts, A., Quintens, R., Tabury, K., Michaux, A., Janssen, A., Neefs, M., Ernst, E., Dieriks, B., Lee, R., De Vos, W. H., Lambert, C., Van Oostveldt, P., Baatout, S."Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation". International Journal of Molecular Medicine 34.4 (2014): 1124-1132.
Chicago
Beck, M., Rombouts, C., Moreels, M., Aerts, A., Quintens, R., Tabury, K., Michaux, A., Janssen, A., Neefs, M., Ernst, E., Dieriks, B., Lee, R., De Vos, W. H., Lambert, C., Van Oostveldt, P., Baatout, S."Modulation of gene expression in endothelial cells in response to high LET nickel ion irradiation". International Journal of Molecular Medicine 34, no. 4 (2014): 1124-1132. https://doi.org/10.3892/ijmm.2014.1893
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