Guanosine exerts neuroprotective effects by reversing mitochondrial dysfunction in a cellular model of Parkinson's disease

  • Authors:
    • Da-Wei Li
    • Min Yao
    • Yan-Hua Dong
    • Min-Na Tang
    • Wei Chen
    • Guang-Ren Li
    • Bi-Quan Sun
  • View Affiliations

  • Published online on: August 19, 2014     https://doi.org/10.3892/ijmm.2014.1904
  • Pages: 1358-1364
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Abstract

The mitochondria are the most important cytoplasmic organelles in determining cell survival and death. Mitochondrial dysfunction leads to a wide range of disorders, including neurodegenerative diseases. The central events in the mitochondrial‑dependent cell death pathway are the activation of the mitochodrial permeability transition pore (mPTP) and the disruption of mitochondrial membrane potential, which cause the release of apoptogenic molecules and finally lead to cell death. This is thought to be at least partly responsible for the loss of dopaminergic neurons in Parkinson's disease (PD); thus, the attenuation of mitochondrial dysfunction may contribute to alleviating the severity and progression of this disease. Guanosine is a pleiotropic molecule affecting multiple cellular processes, including cellular growth, differentiation and survival. Its protective effects on the central nervous system and and on several cell types by inhibiting apoptosis have been shown in a number of pathological conditions. This study aimed to analyze the ability of guanosine to protect neuronal PC12 cells from the toxicity induced by 1-methyl-4-phenylpyridinium (MPP+), the active metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), which mediates selective damage to dopaminergic neurons and causes irreversible Parkinson-like symptoms in humans and primates. Our results demonstrated that the apoptosis of PC12 cells induced by MPP+ was significantly prevented by pre-treatment for 3 h with guanosine. In addition, guanosine attenuated the MPP+-induced collapse of mitochondrial transmembrane potential and prevented the sebsequent activation of caspase-3, thereby protecting dopaminergic neurons against mitochondrial stress-induced damage.
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November-2014
Volume 34 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li D, Yao M, Dong Y, Tang M, Chen W, Li G and Sun B: Guanosine exerts neuroprotective effects by reversing mitochondrial dysfunction in a cellular model of Parkinson's disease. Int J Mol Med 34: 1358-1364, 2014
APA
Li, D., Yao, M., Dong, Y., Tang, M., Chen, W., Li, G., & Sun, B. (2014). Guanosine exerts neuroprotective effects by reversing mitochondrial dysfunction in a cellular model of Parkinson's disease. International Journal of Molecular Medicine, 34, 1358-1364. https://doi.org/10.3892/ijmm.2014.1904
MLA
Li, D., Yao, M., Dong, Y., Tang, M., Chen, W., Li, G., Sun, B."Guanosine exerts neuroprotective effects by reversing mitochondrial dysfunction in a cellular model of Parkinson's disease". International Journal of Molecular Medicine 34.5 (2014): 1358-1364.
Chicago
Li, D., Yao, M., Dong, Y., Tang, M., Chen, W., Li, G., Sun, B."Guanosine exerts neuroprotective effects by reversing mitochondrial dysfunction in a cellular model of Parkinson's disease". International Journal of Molecular Medicine 34, no. 5 (2014): 1358-1364. https://doi.org/10.3892/ijmm.2014.1904