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Article

Angiopoietin-like protein 2 negatively regulated by microRNA-25 contributes to the malignant progression of colorectal cancer

  • Authors:
    • Jumei Zhou
    • Jing Wang
    • Shengqi Wu
    • Suyu Zhu
    • Sai Wang
    • Huijun Zhou
    • Xiaoqing Tian
    • Ning Tang
    • Shaolin Nie
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Tumor Surgery, The Affiliated Tumor Hospital of Xiangya Medical School of Central South University, Changsha, Hunan 410013, P.R. China
  • Pages: 1286-1292
    |
    Published online on: August 19, 2014
       https://doi.org/10.3892/ijmm.2014.1909
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Abstract

Angiopoietin-like protein 2 (ANGPTL2) is associated with tumor progression while dysregulation of its expression has been observed in various types of cancer. However, the expression and role of ANGPTL2 remain exclusive in colorectal cancer (CRC). In the present study, we determined the expression levels of ANGPTL2 in CRC tissues and cells. The roles of ANGPTL2 and miR-25 in the migration and invasion of CRC SW620 and HCT-116 cells were also investigated using transwell assays or scratch wound assays. The results showed that ANGPTL2 increased with metastatic progression. Increased ANGPTL2 and decreased microRNA-25 (miR-25) expression were found to coexist in CRC. The functional studies revealed that knockdown of ANGPTL2 reduced colony formation, and the invasive and migratory abilities of human CRC SW620 and HCT-116 cells. Similarly, overexpression of miR-25 resulted in reduced colony formation, invasion and migration in both cell lines. The overexpression of miR-25 led to a decreased ANGPTL2 mRNA and protein expression, whereas the downregulation of miR-25 resulted in increased ANGPTL2 mRNA and protein expression, in SW620 and HCT-116 cells. miR-25 directly targeted ANGPTL2 by binding to its 3'‑UTR, as determined by the dual luciferase reporter assay. To the best of our know­ledge, the results of this study suggest for the first time that the abnormal upregulation of ANGPTL2 in CRC is associated with miR-25 downregulation. Additionally, miR-25‑mediated ANGPTL2 promoted the malignant progression of CRC. The present study provides evidence supporting ANGPTL2 and miR-25 as diagnostic or therapeutic targets for CRC.
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1 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar

2 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

3 

Suda T, Arai F and Shimmura S: Regulation of stem cells in the niche. Cornea. 24:S12–S17. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Yancopoulos GD, Davis S, Gale NW, Rudge JS, Wiegand SJ and Holash J: Vascular-specific growth factors and blood vessel formation. Nature. 407:242–248. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Hato T, Tabata M and Oike Y: The role of angiopoietin-like proteins in angiogenesis and metabolism. Trends Cardiovasc Med. 18:6–14. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Tabata M, Kadomatsu T, Fukuhara S, et al: Angiopoietin-like protein 2 promotes chronic adipose tissue inflammation and obesity-related systemic insulin resistance. Cell Metab. 10:178–188. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Tazume H, Miyata K, Tian Z, et al: Macrophage-derived angiopoietin-like protein 2 accelerates development of abdominal aortic aneurysm. Arterioscler Thromb Vasc Biol. 32:1400–1409. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Okada T, Tsukano H, Endo M, et al: Synoviocyte-derived angiopoietin-like protein 2 contributes to synovial chronic inflammation in rheumatoid arthritis. Am J Pathol. 176:2309–2319. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Aoi J, Endo M, Kadomatsu T, et al: Angiopoietin-like protein 2 is an important facilitator of inflammatory carcinogenesis and metastasis. Cancer Res. 71:7502–7512. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Endo M, Nakano M, Kadomatsu T, et al: Tumor cell-derived angiopoietin-like protein ANGPTL2 is a critical driver of metastasis. Cancer Res. 72:1784–1794. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Sasaki H, Suzuki A, Shitara M, et al: Angiopoietin-like protein ANGPTL2 gene expression is correlated with lymph node metastasis in lung cancer. Oncol Lett. 4:1325–1328. 2012.

12 

Kikuchi R, Tsuda H, Kozaki K, et al: Frequent inactivation of a putative tumor suppressor, angiopoietin-like protein 2, in ovarian cancer. Cancer Res. 68:5067–5075. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Schetter AJ and Harris CC: Alterations of microRNAs contribute to colon carcinogenesis. Semin Oncol. 38:734–742. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Wu WK, Law PT, Lee CW, et al: MicroRNA in colorectal cancer: from benchtop to bedside. Carcinogenesis. 32:247–253. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Li Q, Zou C, Zou C, et al: MicroRNA-25 functions as a potential tumor suppressor in colon cancer by targeting Smad7. Cancer Lett. 335:168–174. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Arocho A, Chen B, Ladanyi M and Pan Q: Validation of the 2-DeltaDeltaCt calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts. Diagn Mol Pathol. 15:56–61. 2006.PubMed/NCBI

17 

Jiang X, Yue J, Lu H, et al: Inhibition of filamin-A reduces cancer metastatic potential. Int J Biol Sci. 9:67–77. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Aspenström P, Fransson A and Saras J: Rho GTPases have diverse effects on the organization of the actin filament system. Biochem J. 377:327–337. 2004.PubMed/NCBI

19 

Saadoun S, Papadopoulos MC, Hara-Chikuma M and Verkman AS: Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption. Nature. 434:786–792. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Lai AZ, Durrant M, Zuo D, Ratcliffe CD and Park M: Met kinase-dependent loss of the E3 ligase Cbl in gastric cancer. J Biol Chem. 287:8048–8059. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Baldwin A, Huh KW and Munger K: Human papillomavirus E7 oncoprotein dysregulates steroid receptor coactivator 1 localization and function. J Virol. 80:6669–6677. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Hill R, Calvopina JH, Kim C, et al: PTEN loss accelerates KrasG12D-induced pancreatic cancer development. Cancer Res. 70:7114–7124. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Woods NT, Yamaguchi H, Lee FY, Bhalla KN and Wang HG: Anoikis, initiated by Mcl-1 degradation and Bim induction, is deregulated during oncogenesis. Cancer Res. 67:10744–10752. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Kadera BE, Li L, Toste PA, et al: MicroRNA-21 in pancreatic ductal adenocarcinoma tumor-associated fibroblasts promotes metastasis. PLoS One. 8:e719782013. View Article : Google Scholar : PubMed/NCBI

25 

Dahlhaus M, Schult C, Lange S, Freund M and Junghanss C: MicroRNA 181a influences the expression of HMGB1 and CD4 in acute Leukemias. Anticancer Res. 33:445–452. 2013.PubMed/NCBI

26 

Sandhu R, Rivenbark AG and Coleman WB: Loss of post-transcriptional regulation of DNMT3b by microRNAs: A possible molecular mechanism for the hypermethylation defect observed in a subset of breast cancer cell lines. Int J Oncol. 41:721–732. 2012.PubMed/NCBI

27 

Fang JY, Lu J, Chen YX and Yang L: Effects of DNA methylation on expression of tumor suppressor genes and proto-oncogene in human colon cancer cell lines. World J Gastroenterol. 9:1976–1980. 2003.PubMed/NCBI

28 

Pakneshan P, Szyf M and Rabbani SA: Methylation and inhibition of expression of uPA by the RAS oncogene: divergence of growth control and invasion in breast cancer cells. Carcinogenesis. 26:557–564. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Chien CH and Chow SN: Point mutation of the ras oncogene in human ovarian cancer. DNA Cell Biol. 12:623–627. 1993. View Article : Google Scholar : PubMed/NCBI

30 

Beger M, Butz K, Denk C, Williams T, Hurst HC and Hoppe-Seyler F: Expression pattern of AP-2 transcription factors in cervical cancer cells and analysis of their influence on human papillomavirus oncogene transcription. J Mol Med (Berl). 79:314–320. 2001. View Article : Google Scholar

31 

Patel JB, Appaiah HN, Burnett RM, et al: Control of EVI-1 oncogene expression in metastatic breast cancer cells through microRNA miR-22. Oncogene. 30:1290–1301. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Shenouda SK and Alahari SK: MicroRNA function in cancer: oncogene or a tumor suppressor? Cancer Metastasis Rev. 28:369–378. 2009.PubMed/NCBI

33 

Lukiw WJ and Alexandrov PN: Regulation of complement factor H (CFH) by multiple miRNAs in Alzheimer’s disease (AD) brain. Mol Neurobiol. 46:11–19. 2012.PubMed/NCBI

34 

Esposito F, Tornincasa M, Pallante P, et al: Down-regulation of the miR-25 and miR-30d contributes to the development of anaplastic thyroid carcinoma targeting the polycomb protein EZH2. J Clin Endocrinol Metab. 97:E710–E718. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Razumilava N, Bronk SF, Smoot RL, et al: miR-25 targets TNF-related apoptosis inducing ligand (TRAIL) death receptor-4 and promotes apoptosis resistance in cholangiocarcinoma. Hepatology. 55:465–475. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Zhang H, Zuo Z, Lu X, Wang L, Wang H and Zhu Z: miR-25 regulates apoptosis by targeting Bim in human ovarian cancer. Oncol Rep. 27:594–598. 2012.PubMed/NCBI

37 

Rodríguez-Aznar E, Barrallo-Gimeno A and Nieto MA: Scratch2 prevents cell cycle re-entry by repressing miR-25 in postmitotic primary neurons. J Neurosci. 33:5095–5105. 2013.PubMed/NCBI

38 

Lu D, Davis MP, Abreu-Goodger C, et al: miR-25 regulates Wwp2 and Fbxw7 and promotes reprogramming of mouse fibroblast cells to iPSCs. PLoS One. 7:e409382012. View Article : Google Scholar : PubMed/NCBI

39 

Zhu Z, He J, Jia X, et al: MicroRNA-25 functions in regulation of pigmentation by targeting the transcription factor MITF in alpaca (Lama pacos) skin melanocytes. Domest Anim Endocrinol. 38:200–209. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Wang J, Wu S, Zhu S, Wang S, Zhou H, Tian X, Tang N and Nie S: Angiopoietin-like protein 2 negatively regulated by microRNA-25 contributes to the malignant progression of colorectal cancer. Int J Mol Med 34: 1286-1292, 2014.
APA
Zhou, J., Wang, J., Wu, S., Zhu, S., Wang, S., Zhou, H. ... Nie, S. (2014). Angiopoietin-like protein 2 negatively regulated by microRNA-25 contributes to the malignant progression of colorectal cancer. International Journal of Molecular Medicine, 34, 1286-1292. https://doi.org/10.3892/ijmm.2014.1909
MLA
Zhou, J., Wang, J., Wu, S., Zhu, S., Wang, S., Zhou, H., Tian, X., Tang, N., Nie, S."Angiopoietin-like protein 2 negatively regulated by microRNA-25 contributes to the malignant progression of colorectal cancer". International Journal of Molecular Medicine 34.5 (2014): 1286-1292.
Chicago
Zhou, J., Wang, J., Wu, S., Zhu, S., Wang, S., Zhou, H., Tian, X., Tang, N., Nie, S."Angiopoietin-like protein 2 negatively regulated by microRNA-25 contributes to the malignant progression of colorectal cancer". International Journal of Molecular Medicine 34, no. 5 (2014): 1286-1292. https://doi.org/10.3892/ijmm.2014.1909
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Wang J, Wu S, Zhu S, Wang S, Zhou H, Tian X, Tang N and Nie S: Angiopoietin-like protein 2 negatively regulated by microRNA-25 contributes to the malignant progression of colorectal cancer. Int J Mol Med 34: 1286-1292, 2014.
APA
Zhou, J., Wang, J., Wu, S., Zhu, S., Wang, S., Zhou, H. ... Nie, S. (2014). Angiopoietin-like protein 2 negatively regulated by microRNA-25 contributes to the malignant progression of colorectal cancer. International Journal of Molecular Medicine, 34, 1286-1292. https://doi.org/10.3892/ijmm.2014.1909
MLA
Zhou, J., Wang, J., Wu, S., Zhu, S., Wang, S., Zhou, H., Tian, X., Tang, N., Nie, S."Angiopoietin-like protein 2 negatively regulated by microRNA-25 contributes to the malignant progression of colorectal cancer". International Journal of Molecular Medicine 34.5 (2014): 1286-1292.
Chicago
Zhou, J., Wang, J., Wu, S., Zhu, S., Wang, S., Zhou, H., Tian, X., Tang, N., Nie, S."Angiopoietin-like protein 2 negatively regulated by microRNA-25 contributes to the malignant progression of colorectal cancer". International Journal of Molecular Medicine 34, no. 5 (2014): 1286-1292. https://doi.org/10.3892/ijmm.2014.1909
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