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International Journal of Molecular Medicine
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Article

Adhesion signaling promotes protease‑driven polyploidization of glioblastoma cells

  • Authors:
    • Javier Mercapide
    • Aurelio Lorico
  • View Affiliations / Copyright

    Affiliations: Cancer Research Center, Roseman University of Health Sciences, Las Vegas, NV, USA
  • Pages: 1365-1371
    |
    Published online on: August 25, 2014
       https://doi.org/10.3892/ijmm.2014.1913
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Abstract

An increase in ploidy (polyploidization) causes genomic instability in cancer. However, the determinants for the increased DNA content of cancer cells have not yet been fully elucidated. In the present study, we investigated whether adhesion induces polyploidization in human U87MG glioblastoma cells. For this purpose, we employed expression vectors that reported transcriptional activation by signaling networks implicated in cancer. Signaling activation induced by intercellular integrin binding elicited both extracellular signal‑regulated kinase (ERK) and Notch target transcription. Upon the prolonged activation of both ERK and Notch target transcription induced by integrin binding to adhesion protein, cell cultures accumulated polyploid cells, as determined by cell DNA content distribution analysis and the quantification of polynucleated cells. This linked the transcriptional activation induced by integrin adhesion to the increased frequency of polyploidization. Accordingly, the inhibition of signaling decreased the extent of polyploidization mediated by protease‑driven intracellular invasion. Therefore, the findings of this study indicate that integrin adhesion induces polyploidization through the stimulation of glioblastoma cell invasiveness.
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Copy and paste a formatted citation
Spandidos Publications style
Mercapide J and Lorico A: Adhesion signaling promotes protease‑driven polyploidization of glioblastoma cells. Int J Mol Med 34: 1365-1371, 2014.
APA
Mercapide, J., & Lorico, A. (2014). Adhesion signaling promotes protease‑driven polyploidization of glioblastoma cells. International Journal of Molecular Medicine, 34, 1365-1371. https://doi.org/10.3892/ijmm.2014.1913
MLA
Mercapide, J., Lorico, A."Adhesion signaling promotes protease‑driven polyploidization of glioblastoma cells". International Journal of Molecular Medicine 34.5 (2014): 1365-1371.
Chicago
Mercapide, J., Lorico, A."Adhesion signaling promotes protease‑driven polyploidization of glioblastoma cells". International Journal of Molecular Medicine 34, no. 5 (2014): 1365-1371. https://doi.org/10.3892/ijmm.2014.1913
Copy and paste a formatted citation
x
Spandidos Publications style
Mercapide J and Lorico A: Adhesion signaling promotes protease‑driven polyploidization of glioblastoma cells. Int J Mol Med 34: 1365-1371, 2014.
APA
Mercapide, J., & Lorico, A. (2014). Adhesion signaling promotes protease‑driven polyploidization of glioblastoma cells. International Journal of Molecular Medicine, 34, 1365-1371. https://doi.org/10.3892/ijmm.2014.1913
MLA
Mercapide, J., Lorico, A."Adhesion signaling promotes protease‑driven polyploidization of glioblastoma cells". International Journal of Molecular Medicine 34.5 (2014): 1365-1371.
Chicago
Mercapide, J., Lorico, A."Adhesion signaling promotes protease‑driven polyploidization of glioblastoma cells". International Journal of Molecular Medicine 34, no. 5 (2014): 1365-1371. https://doi.org/10.3892/ijmm.2014.1913
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