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Transforming growth factor β regulates β-catenin expression in lung fibroblast through NF-κB dependent pathway

  • Authors:
    • Jian Li
    • Gang Wang
    • Xia Sun
  • View Affiliations / Copyright

    Affiliations: Jinan Centre for Disease Control and Prevention, Jinan, Shandong 250001, P.R. China, Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical college, Xuzhou, Jiangsu 223002, P.R. China, Department of Pediatrics, The University of Chicago, Chicago, IL 60637, USA
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1219-1224
    |
    Published online on: August 27, 2014
       https://doi.org/10.3892/ijmm.2014.1916
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Abstract

β-catenin contributes to the pathogenesis of lung fibrosis. However, the expression of β-catenin in fibroblasts under fibrotic conditions has not been studied. We investigated the expression of β-catenin in lung fibroblasts from bleomycin (BLM)‑challenged mice and human lung fibroblasts treated with transforming growth factor β (TGF-β) or lysophosphatidic acid (LPA) by western blot analysis. The result showed that the expression of β-catenin was significantly increased in lung fibrotic foci and lung fibroblasts from bleomycin‑challenged mice. TGF-β stimulated β-catenin expression and induced differentiation in human lung fibroblasts in vitro. Pretreatment of the NF-κB activation inhibitor attenuated the TGF-β‑induced expression of β-catenin and differentiation in human lung fibroblasts. Similarly, LPA induced β-catenin expression in human lung fibroblasts, and pre-treatment of the neutralized anti-TGF-β antibody attenuated the LPA‑induced expression of β-catenin and differentiation in human lung fibroblasts. The results suggested that β-catenin expression is upregulated in lung fibroblast during differentiation, and that TGF-β induced β-catenin expression in human lung fibroblasts through the activation of NF-κB.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Wang G and Sun X: Transforming growth factor β regulates β-catenin expression in lung fibroblast through NF-κB dependent pathway. Int J Mol Med 34: 1219-1224, 2014.
APA
Li, J., Wang, G., & Sun, X. (2014). Transforming growth factor β regulates β-catenin expression in lung fibroblast through NF-κB dependent pathway. International Journal of Molecular Medicine, 34, 1219-1224. https://doi.org/10.3892/ijmm.2014.1916
MLA
Li, J., Wang, G., Sun, X."Transforming growth factor β regulates β-catenin expression in lung fibroblast through NF-κB dependent pathway". International Journal of Molecular Medicine 34.5 (2014): 1219-1224.
Chicago
Li, J., Wang, G., Sun, X."Transforming growth factor β regulates β-catenin expression in lung fibroblast through NF-κB dependent pathway". International Journal of Molecular Medicine 34, no. 5 (2014): 1219-1224. https://doi.org/10.3892/ijmm.2014.1916
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Wang G and Sun X: Transforming growth factor β regulates β-catenin expression in lung fibroblast through NF-κB dependent pathway. Int J Mol Med 34: 1219-1224, 2014.
APA
Li, J., Wang, G., & Sun, X. (2014). Transforming growth factor β regulates β-catenin expression in lung fibroblast through NF-κB dependent pathway. International Journal of Molecular Medicine, 34, 1219-1224. https://doi.org/10.3892/ijmm.2014.1916
MLA
Li, J., Wang, G., Sun, X."Transforming growth factor β regulates β-catenin expression in lung fibroblast through NF-κB dependent pathway". International Journal of Molecular Medicine 34.5 (2014): 1219-1224.
Chicago
Li, J., Wang, G., Sun, X."Transforming growth factor β regulates β-catenin expression in lung fibroblast through NF-κB dependent pathway". International Journal of Molecular Medicine 34, no. 5 (2014): 1219-1224. https://doi.org/10.3892/ijmm.2014.1916
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