Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
November-2014 Volume 34 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2014 Volume 34 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Homocysteine reduces protein S-nitrosylation in endothelium

  • Authors:
    • Yulong Chen
    • Sihai Zhao
    • Yanli Wang
    • Yafeng Li
    • Liang  Bai
    • Ruihan Liu
    • Jianglin Fan
    • Enqi Liu
  • View Affiliations / Copyright

    Affiliations: Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University Cardiovascular Research Center, Xi'an, Shaanxi 710061, P.R. China, Research Institute of Atherosclerotic Disease, Xi'an Jiaotong University Cardiovascular Research Center, Xi'an, Shaanxi 710061, P.R. China, Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi 409-3898, Japan
  • Pages: 1277-1285
    |
    Published online on: September 4, 2014
       https://doi.org/10.3892/ijmm.2014.1920
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hyperhomocysteinemia (HHcy) is a risk factor for cardiovascular disease. The S-nitrosylation of proteins is involved in the regulation of cardiovascular functions. However, whether homocysteine (Hcy) impairs vascular functions through the inhibition of protein S-nitrosylation in the endothelium remains to be determined. The experiments were performed in human umbilical vein endothelial cells (HUVECs). Male Sprague‑Dawley rats, with or without administration of L-methionine, were used for the in vivo validation of findings. S-nitrosylation was analyzed using immunofluorescence for nitrosocysteine, and further confirmed by the biotin switch method. The levels of reactive oxygen species (ROS) were detected by 2',7'-dichlorofluorescein diacetate (DCFH-DA) staining. The levels of nitric oxide (ΝΟ) were determined by the nitrate reduction method. Protein expression was analysed by western blot analysis. The activity of nuclear factor κB (NF-κB) was evaluated by an electrophoretic mobility shift assay (EMSA). The levels of plasma Hcy were measured by ELISA. The results showed that Hcy significantly reduced the levels of protein S-nitrosylation in HUVECs and endothelial S-nitrosylation of aorta. This reduction of protein S-nitrosylation was accompanied by increasing ROS, decreasing phosphorylation levels of Akt and endothelial nitric oxide synthase (eNOS), and reduced levels of nitric oxide in HUVECs. In addition, it was found that Hcy increased the protein expression of vascular cell adhesion molecule-1 by attenuating the cytoplasm S-nitrosylation of NF-κB (p65). These data suggested that Hcy impairs endothelial functions by inhibiting endothelial protein S-nitrosylation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Martínez-Ruiz A and Lamas S: Signalling by NO-induced protein S-nitrosylation and S-glutathionylation: convergences and divergences. Cardiovasc Res. 75:220–228. 2007.PubMed/NCBI

2 

Lima B, Forrester MT, Hess DT and Stamler JS: S-nitrosylation in cardiovascular signaling. Circ Res. 106:633–646. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Chakrabarti S, Lekontseva O, Peters A and Davidge ST: 17beta-Estradiol induces protein S-nitrosylation in the endothelium. Cardiovasc Res. 85:796–805. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Sun J, Picht E, Ginsburg KS, Bers DM, Steenbergen C and Murphy E: Hypercontractile female hearts exhibit increased S-nitrosylation of the L-type Ca2t channel alpha1 subunit and reduced ischemia/reperfusion injury. Circ Res. 98:403–411. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Selemidis S, Dusting GJ, Peshavariya H, Kemp-Harper BK and Drummond GR: Nitric oxide suppresses NADPH oxidase-dependent superoxide production by S-nitrosylation in human endothelial cells. Cardiovasc Res. 75:349–358. 2007. View Article : Google Scholar

6 

Kang-Decker N, Cao S, Chatterjee S, Yao J, Egan LJ, Semela D, Mukhopadhyay D and Shah V: Nitric oxide promotes endothelial cell survival signaling through S-nitrosylation and activation of dynamin-2. J Cell Sci. 120:492–501. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Marshall HE and Stamler JS: Inhibition of NF-kappa B by S-nitrosylation. Biochemistry. 40:1688–1693. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Hoffmann J, Haendeler J, Zeiher AM and Dimmeler S: TNFalpha and oxLDL reduce protein S-nitrosylation in endothelial cells. J Biol Chem. 276:41383–41387. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Wadham C, Parker A, Wang L and Xia P: High glucose attenuates protein S-nitrosylation in endothelial cells: role of oxidative stress. Diabetes. 58:2715–2721. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Hoffmann J, Dimmeler S and Haendeler J: Shear stress increases the amount of S-nitrosylated molecules in endothelial cells: important role for signal transduction. FEBS Lett. 551:153–158. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Chen SC, Huang B, Liu YC, Shyu KG, Lin PY and Wang DL: Acute hypoxia enhances proteins’ S-nitrosylation in endothelial cells. Biochem Biophys Res Commun. 377:1274–1278. 2008.

12 

Hankey GJ and Eikelboom JW: Homocysteine and vascular disease. Lancet. 354:407–413. 1999. View Article : Google Scholar

13 

Suematsu N, Ojaimi C, Kinugawa S, et al: Hyperhomocysteinemia alters cardiac substrate metabolism by impairing nitric oxide bioavailability through oxidative stress. Circulation. 115:255–262. 2007. View Article : Google Scholar

14 

Ungvari Z, Sarkadi-Nagy E, Bagi Z, Szollár L and Koller A: Simultaneously increased TxA(2) activity in isolated arterioles and platelets of rats with hyperhomocysteinemia. Arterioscler Thromb Vasc Biol. 20:1203–1208. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Forrester MT, Foster MW, Benhar M and Stamler JS: Detection of protein S-nitrosylation with the biotin-switch technique. Free Radic Biol Med. 46:119–126. 2009.PubMed/NCBI

16 

Ota H, Eto M, Kano MR, Ogawa S, Iijima K, Akishita M and Ouchi Y: Cilostazol inhibits oxidative stress-induced premature senescence via upregulation of Sirt1 in human endothelial cells. Arterioscler Thromb Vasc Biol. 28:1634–1639. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Wu Y, Zhang W, Liu W, Zhuo X, Zhao Z and Yuan Z: The double-faced metabolic and inflammatory effects of standard drug therapy in patients after percutaneous treatment with drug-eluting stent. Atherosclerosis. 215:170–175. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Michaud SE, Dussault S, Groleau J, Haddad P and Rivard A: Cigarette smoke exposure impairs VEGF-induced endothelial cell migration: role of NO and reactive oxygen species. J Mol Cell Cardiol. 41:275–284. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Lan TH, Xu ZW, Wang Z, Wu YL, Wu WK and Tan HM: Ginsenoside Rb1 prevents homocysteine-induced endothelial dysfunction via PI3K/Akt activation and PKC inhibition. Biochem Pharmacol. 82:148–155. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Thibeault S, Rautureau Y, Oubaha M, Faubert D, Wilkes BC, Delisle C and Gratton JP: S-nitrosylation of beta-catenin by eNOS-derived NO promotes VEGF-induced endothelial cell permeability. Mol Cell. 39:468–476. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Pi X, Wu Y, Ferguson JE III, Portbury AL and Patterson C: SDF-1alpha stimulates JNK3 activity via eNOS-dependent nitrosylation of MKP7 to enhance endothelial migration. Proc Natl Acad Sci USA. 106:5675–5680. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Oliveira CJ, Curcio MF, Moraes MS, Tsujita M, Travassos LR, Stern A and Monteiro HP: The low molecular weight S-nitrosothiol, S-nitroso-N-acetylpenicillamine, promotes cell cycle progression in rabbit aortic endothelial cells. Nitric Oxide. 18:241–255. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Santhanam L, Tuday EC, Webb AK, et al: Decreased S-nitrosylation of tissue transglutaminase contributes to age-related increases invascular stiffness. Circ Res. 107:117–125. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Martínez-Ruiz A and Lamas S: Detection and proteomic identification of S-nitrosylated proteins in endothelial cells. Arch Biochem Biophys. 423:192–199. 2004.

25 

McCully KS: Chemical pathology of homocysteine. IV Excitotoxicity, oxidative stress, endothelial dysfunction, and inflammation. Ann Clin Lab Sci. 39:219–232. 2009.PubMed/NCBI

26 

Chavakis E, Dernbach E, Hermann C, Mondorf UF, Zeiher AM and Dimmeler S: Oxidized LDL inhibits vascular endothelial growth factor-induced endothelial cell migration by an inhibitory effect on the Akt/endothelial nitric oxide synthase pathway. Circulation. 103:2102–2107. 2001. View Article : Google Scholar

27 

Dimmeler S, Fleming I, Fisslthaler B, Hermann C, Busse R and Zeiher AM: Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation. Nature. 399:601–605. 1999. View Article : Google Scholar : PubMed/NCBI

28 

Carluccio MA, Ancora MA, Massaro M, et al: Homocysteine induces VCAM-1 gene expression through NF-kappaB and NAD(P)H oxidase activation: protective role of Mediterranean diet polyphenolic antioxidants. Am J Physiol Heart Circ Physiol. 293:H2344–H2354. 2007. View Article : Google Scholar

29 

Au-Yeung KK, Woo CW, Sung FL, Yip JC, Siow YL and OK: Hyperhomocysteinemia activates nuclear factor-kappaB in endothelial cells via oxidative stress. Circ Res. 94:28–36. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Kelleher ZT, Matsumoto A, Stamler JS and Marshall HE: NOS2 regulation of NF-kappaB by S-nitrosylation of p65. J Biol Chem. 282:30667–30672. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Marshall HE, Hess DT and Stamler JS: S-nitrosylation: physiological regulation of NF-kappaB. Proc Natl Acad Sci USA. 101:8841–8842. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Reynaert NL, Ckless K, Korn SH, et al: Nitric oxide represses inhibitory kappaB kinase through S-nitrosylation. Proc Natl Acad Sci USA. 101:8945–8950. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Zhao S, Wang Y, Li Y, Bai L, Liu R, Fan J and Liu E: Homocysteine reduces protein S-nitrosylation in endothelium. Int J Mol Med 34: 1277-1285, 2014.
APA
Chen, Y., Zhao, S., Wang, Y., Li, Y., Bai, L., Liu, R. ... Liu, E. (2014). Homocysteine reduces protein S-nitrosylation in endothelium. International Journal of Molecular Medicine, 34, 1277-1285. https://doi.org/10.3892/ijmm.2014.1920
MLA
Chen, Y., Zhao, S., Wang, Y., Li, Y., Bai, L., Liu, R., Fan, J., Liu, E."Homocysteine reduces protein S-nitrosylation in endothelium". International Journal of Molecular Medicine 34.5 (2014): 1277-1285.
Chicago
Chen, Y., Zhao, S., Wang, Y., Li, Y., Bai, L., Liu, R., Fan, J., Liu, E."Homocysteine reduces protein S-nitrosylation in endothelium". International Journal of Molecular Medicine 34, no. 5 (2014): 1277-1285. https://doi.org/10.3892/ijmm.2014.1920
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Zhao S, Wang Y, Li Y, Bai L, Liu R, Fan J and Liu E: Homocysteine reduces protein S-nitrosylation in endothelium. Int J Mol Med 34: 1277-1285, 2014.
APA
Chen, Y., Zhao, S., Wang, Y., Li, Y., Bai, L., Liu, R. ... Liu, E. (2014). Homocysteine reduces protein S-nitrosylation in endothelium. International Journal of Molecular Medicine, 34, 1277-1285. https://doi.org/10.3892/ijmm.2014.1920
MLA
Chen, Y., Zhao, S., Wang, Y., Li, Y., Bai, L., Liu, R., Fan, J., Liu, E."Homocysteine reduces protein S-nitrosylation in endothelium". International Journal of Molecular Medicine 34.5 (2014): 1277-1285.
Chicago
Chen, Y., Zhao, S., Wang, Y., Li, Y., Bai, L., Liu, R., Fan, J., Liu, E."Homocysteine reduces protein S-nitrosylation in endothelium". International Journal of Molecular Medicine 34, no. 5 (2014): 1277-1285. https://doi.org/10.3892/ijmm.2014.1920
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team