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Article

Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells

  • Authors:
    • Jialin Pan
    • Juhong Zhang
    • Xingwei Zhang
    • Xi Zhou
    • Shengyue Lu
    • Xiaoyan Huang
    • Jiayu Shao
    • Guoqiang Lou
    • Deye Yang
    • Yong-Jian Geng
  • View Affiliations / Copyright

    Affiliations: Division of Cardiology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Division of Cardiology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang 310015, P.R. China, Division of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, The Center for Cardiovascular Biology and Atherosclerosis Research, The University of Texas School of Medicine at Houston, Houston, TX 77030, USA
  • Pages: 1381-1387
    |
    Published online on: September 16, 2014
       https://doi.org/10.3892/ijmm.2014.1935
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Abstract

Angiotensin II (Ang II) has been proven to induce epithelial-mesenchymal transition (EMT). The aim of the present study was to determine the role of microRNA-29b (miR-29b) during Ang II-induced EMT. For this purpose, we used spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto (WKY) rats. The levels of Ang II and its receptor in the kidneys of the SHRs are significantly higher than those in the age-matched WKY rats. As shown by RT-qPCR, the expression of miR-29b in the renal cortex was lower in the SHRs than in the WKY rats. For in vitro experiments, NRK-52E renal tubular epithelial cells were treated with 10-7 M Ang II; we found that the expression of miR-29b was decreased in the cells treated with Ang II. In addition, transfection of the NRK-52E cells with miR-29b inhibitor led to the downregulation of miR-29b in these cells, and increased the expression of transforming growth factor (TGF)-β, α-smooth muscle actin (α-SMA) and collagen I (Col I). Similar results were observed with the induction of Ang II expression in the NRK-52E cells. By contrast, the upregulation of miR-29b by transfection with miR-29b mimics inhibited the overexpression of these genes induced by Ang II. These results suggest that miR-29b plays an important role in Ang II-induced EMT.
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1 

Cesana M, Cacchiarelli D, Legnini I, Santini T, Sthandier O, Chinappi M, Tramontano A and Bozzoni I: A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA. Cell. 147:358–369. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Choi YJ, Lin CP, Ho JJ, He X, Okada N, Bu P, Zhong Y, Kim SY, Bennett MJ, Chen C, Ozturk A, Hicks GG, Hannon GJ and He L: miR-34 miRNAs provide a barrier for somatic cell reprogramming. Nat Cell Biol. 13:1353–1360. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Wang G, Kwan BC, Lai FM, Chow KM, Li PK and Szeto CC: Urinary miR-21, miR-29, and miR-93: novel biomarkers of fibrosis. Am J Nephrol. 36:412–418. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Lekgabe ED, Kiriazis H, Zhao C, Xu Q, Moore XL, Su Y, Bathgate RA, Du XJ and Samuel CS: Relaxin reverses cardiac and renal fibrosis in spontaneously hypertensive rats. Hypertension. 46:412–418. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Cheng HF, Wang JL, Vinson GP and Harris RC: Young SHR express increased type 1 angiotensin II receptors in renal proximal tubule. Am J Physiol. 274:F10–F17. 1998.PubMed/NCBI

6 

Matsushima Y, Kawamura M, Akabane S, Imanishi M, Kuramochi M, Ito K and Omae T: Increases in renal angiotensin II content and tubular angiotensin II receptors in prehypertensive spontaneously hypertensive rats. J Hypertens. 6:791–796. 1988. View Article : Google Scholar : PubMed/NCBI

7 

Mezzano SA, Ruiz-Ortega M and Egido J: Angiotensin II and renal fibrosis. Hypertension. 38:635–638. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Jimenez E, Perez de la Blanca E, Urso L, Gonzalez I, Salas J and Montiel M: Angiotensin II induces MMP 2 activity via FAK/JNK pathway in human endothelial cells. Biochem Biophys Res Commun. 380:769–774. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J and Ruiz-Ortega M: Angiotensin II contributes to renal fibrosis independently of Notch pathway activation. PLoS One. 7:e404902012. View Article : Google Scholar : PubMed/NCBI

11 

Pons M, Cousins SW, Alcazar O, Striker GE and Marin-Castano ME: Angiotensin II-induced MMP-2 activity and MMP-14 and basigin protein expression are mediated via the angiotensin II receptor type 1-mitogen-activated protein kinase 1 pathway in retinal pigment epithelium: implications for age-related macular degeneration. Am J Pathol. 178:2665–2681. 2011. View Article : Google Scholar

12 

Zhong J, Guo D, Chen CB, Wang W, Schuster M, Loibner H, Penninger JM, Scholey JW, Kassiri Z and Oudit GY: Prevention of angiotensin II-mediated renal oxidative stress, inflammation, and fibrosis by angiotensin-converting enzyme 2. Hypertension. 57:314–322. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Cushing L, Kuang PP, Qian J, Shao F, Wu J, Little F, Thannickal VJ, Cardoso WV and Lu J: miR-29 is a major regulator of genes associated with pulmonary fibrosis. Am J Respir Cell Mol Biol. 45:287–294. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Qin W, Chung AC, Huang XR, Meng XM, Hui DS, Yu CM, Sung JJ and Lan HY: TGF-β/Smad3 signaling promotes renal fibrosis by inhibiting miR-29. J Am Soc Nephrol. 22:1462–1474. 2011.

15 

Roderburg C, Urban GW, Bettermann K, Vucur M, Zimmermann H, Schmidt S, Janssen J, Koppe C, Knolle P, Castoldi M, Tacke F, Trautwein C and Luedde T: Micro-RNA profiling reveals a role for miR-29 in human and murine liver fibrosis. Hepatology. 53:209–218. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Liu GX, Li YQ, Huang XR, Wei L, Chen HY, Shi YJ, Heuchel RL and Lan HY: Disruption of Smad7 promotes ANG II-mediated renal inflammation and fibrosis via Sp1-TGF-β/Smad3-NF. κB-dependent mechanisms in mice. PLoS One. 8:e535732013.PubMed/NCBI

17 

Han Y, Runge MS and Brasier AR: Angiotensin II induces interleukin-6 transcription in vascular smooth muscle cells through pleiotropic activation of nuclear factor-κB transcription factors. Circ Res. 84:695–703. 1999.PubMed/NCBI

18 

Kranzhofer R, Schmidt J, Pfeiffer CA, Hagl S, Libby P and Kubler W: Angiotensin induces inflammatory activation of human vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 19:1623–1629. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Wang H, Garzon R, Sun H, Ladner KJ, Singh R, Dahlman J, Cheng A, Hall BM, Qualman SJ, Chandler DS, Croce CM and Guttridge DC: NF-κB-YY1-miR-29 regulatory circuitry in skeletal myogenesis and rhabdomyosarcoma. Cancer Cell. 14:369–381. 2008.

20 

Chang TC, Yu D, Lee YS, Wentzel EA, Arking DE, West KM, Dang CV, Thomas-Tikhonenko A and Mendell JT: Widespread microRNA repression by Myc contributes to tumorigenesis. Nat Genet. 40:43–50. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Mott JL, Kurita S, Cazanave SC, Bronk SF, Wemeburg NW and Femandez-Zapico ME: Transcriptional suppression of mir-29b-1/mir-29a promoter by c-Myc, hedgehog, and NF-kappaB. J Cell Biochem. 110:1155–1164. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Sadoshima J and Izumo S: Signal transduction pathways of angiotensin II - induced c-fos gene expression in cardiac myocytes in vitro. Roles of phospholipid-derived second messengers. Circ Res. 73:424–438. 1993. View Article : Google Scholar : PubMed/NCBI

23 

Kopp JB: TGF-β signaling and the renal tubular epithelial cell: too much, too little, and just right. J Am Soc Nephrol. 21:1241–1243. 2010.

24 

Meng XM, Huang XR, Chung AC, Qin W, Shao X, Igarashi P, Ju W, Bottinger EP and Lan HY: Smad2 protects against TGF-β/Smad3-mediated renal fibrosis. J Am Soc Nephrol. 21:1477–1487. 2010.

25 

Chung AC, Yu X and Lan HY: MicroRNA and nephropathy: emerging concepts. Int J Nephrol Renovasc Dis. 6:169–179. 2013.PubMed/NCBI

26 

Patel V and Noureddine L: MicroRNAs and fibrosis. Curr Opin Nephrol Hypertens. 21:410–416. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Fort A, Borel C, Migliavacca E, Antonarakis SE, Fish RJ and Neerman-Arbez M: Regulation of fibrinogen production by microRNAs. Blood. 116:2608–2615. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Villarreal G Jr, Oh DJ, Kang MH and Rhee DJ: Coordinated regulation of extracellular matrix synthesis by the microRNA-29 family in the trabecular meshwork. Invest Ophthalmol Vis Sci. 52:3391–3397. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Kapinas K, Kessler CB and Delany AM: miR-29 suppression of osteonectin in osteoblasts: regulation during differentiation and by canonical Wnt signaling. J Cell Biochem. 108:216–224. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Inoki K, Haneda M, Ishida T, Mori H, Maeda S, Koya D, Sugimoto T and Kikkawa R: Role of mitogen-activated protein kinases as downstream effectors of transforming growth factor-beta in mesangial cells. Kidney Int Suppl. 77:S76–S80. 2000. View Article : Google Scholar : PubMed/NCBI

31 

Luna C, Li G, Qiu J, Epstein DL and Gonzalez P: Cross-talk between miR-29 and transforming growth factor-betas in trabecular meshwork cells. Invest Ophthalmol Vis Sci. 52:3567–3572. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Pan J, Zhang J, Zhang X, Zhou X, Lu S, Huang X, Shao J, Lou G, Yang D, Geng Y, Geng Y, et al: Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells. Int J Mol Med 34: 1381-1387, 2014.
APA
Pan, J., Zhang, J., Zhang, X., Zhou, X., Lu, S., Huang, X. ... Geng, Y. (2014). Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells. International Journal of Molecular Medicine, 34, 1381-1387. https://doi.org/10.3892/ijmm.2014.1935
MLA
Pan, J., Zhang, J., Zhang, X., Zhou, X., Lu, S., Huang, X., Shao, J., Lou, G., Yang, D., Geng, Y."Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells". International Journal of Molecular Medicine 34.5 (2014): 1381-1387.
Chicago
Pan, J., Zhang, J., Zhang, X., Zhou, X., Lu, S., Huang, X., Shao, J., Lou, G., Yang, D., Geng, Y."Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells". International Journal of Molecular Medicine 34, no. 5 (2014): 1381-1387. https://doi.org/10.3892/ijmm.2014.1935
Copy and paste a formatted citation
x
Spandidos Publications style
Pan J, Zhang J, Zhang X, Zhou X, Lu S, Huang X, Shao J, Lou G, Yang D, Geng Y, Geng Y, et al: Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells. Int J Mol Med 34: 1381-1387, 2014.
APA
Pan, J., Zhang, J., Zhang, X., Zhou, X., Lu, S., Huang, X. ... Geng, Y. (2014). Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells. International Journal of Molecular Medicine, 34, 1381-1387. https://doi.org/10.3892/ijmm.2014.1935
MLA
Pan, J., Zhang, J., Zhang, X., Zhou, X., Lu, S., Huang, X., Shao, J., Lou, G., Yang, D., Geng, Y."Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells". International Journal of Molecular Medicine 34.5 (2014): 1381-1387.
Chicago
Pan, J., Zhang, J., Zhang, X., Zhou, X., Lu, S., Huang, X., Shao, J., Lou, G., Yang, D., Geng, Y."Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells". International Journal of Molecular Medicine 34, no. 5 (2014): 1381-1387. https://doi.org/10.3892/ijmm.2014.1935
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