Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells

Pan,Jialin; Zhang,Juhong; Zhang,Xingwei; Zhou,Xi; Lu,Shengyue; Huang,Xiaoyan; Shao,Jiayu; Lou,Guoqiang; Yang,Deye; Geng,Yong-Jian

doi:10.3892/ijmm.2014.1935

Role of microRNA-29b in angiotensin II-induced epithelial-mesenchymal transition in renal tubular epithelial cells

Authors:
- Jialin Pan
- Juhong Zhang
- Xingwei Zhang
- Xi Zhou
- Shengyue Lu
- Xiaoyan Huang
- Jiayu Shao
- Guoqiang Lou
- Deye Yang
- Yong-Jian Geng
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Affiliations: Division of Cardiology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Division of Cardiology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang 310015, P.R. China, Division of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, The Center for Cardiovascular Biology and Atherosclerosis Research, The University of Texas School of Medicine at Houston, Houston, TX 77030, USA
Published online on: September 16, 2014 https://doi.org/10.3892/ijmm.2014.1935
Pages: 1381-1387

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Abstract

Angiotensin II (Ang II) has been proven to induce epithelial-mesenchymal transition (EMT). The aim of the present study was to determine the role of microRNA-29b (miR-29b) during Ang II-induced EMT. For this purpose, we used spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto (WKY) rats. The levels of Ang II and its receptor in the kidneys of the SHRs are significantly higher than those in the age-matched WKY rats. As shown by RT-qPCR, the expression of miR-29b in the renal cortex was lower in the SHRs than in the WKY rats. For in vitro experiments, NRK-52E renal tubular epithelial cells were treated with 10-7 M Ang II; we found that the expression of miR-29b was decreased in the cells treated with Ang II. In addition, transfection of the NRK-52E cells with miR-29b inhibitor led to the downregulation of miR-29b in these cells, and increased the expression of transforming growth factor (TGF)-β, α-smooth muscle actin (α-SMA) and collagen I (Col I). Similar results were observed with the induction of Ang II expression in the NRK-52E cells. By contrast, the upregulation of miR-29b by transfection with miR-29b mimics inhibited the overexpression of these genes induced by Ang II. These results suggest that miR-29b plays an important role in Ang II-induced EMT.

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