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Article

Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis in human nasopharyngeal carcinoma cells

  • Authors:
    • Zhixiong Long
    • Bin Wang
    • Dan Tao
    • Ying Huang
    • Zezhang Tao
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Otolaryngology (ENT), The Fifth Hospital of Wuhan, Wuhan, Hubei 430051, P.R. China, Department of Oncology, The Fifth Hospital of Wuhan, Wuhan, Hubei 430051, P.R. China
  • Pages: 1388-1394
    |
    Published online on: September 17, 2014
       https://doi.org/10.3892/ijmm.2014.1937
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Abstract

Nasopharyngeal carcinoma (NPC) is a relatively radiosensitive disease. However, the therapeutic effects of radiotherapy are not always satisfactory due to radioresistance. The hypofractionated schema is currently widely used in clinical practice. In the present study, we investigated the effects of hypofractionated radiotherapy on NPC cells and explored the mechanisms involved. In addition, we aimed to determine the role of miR-34a in the effects of hypofractionated radiotherapy and whether these effects occur in a p53-dependent manner. For this purpose, we used CNE1 and CNE2 NPC cells which were subjected to hyperfractionated and hypofractionated radiotherapy. The viability of the cells was measured by MTT assay and acridine orange (AO) and ethidium bromide (EB) staining was used to observe morphological changes. In addition, Annexin V-propidium iodide (PI) staining and flow cytometry were used to determine the number of apoptotic cells and mRNA and protein expression was measured by qPCR and western blot analysis, respectively. The results revealed that hypofractionated radiotherapy enhanced apoptosis and increased the expression of miR-34a and p53 in the NPC cells. In addition, it stimulated p53 promoter activity and downregulated the protein expression of c-Myc in the human NPC cells. Furthermore, the knockdown of miR-34a suppressed the growth inhibitory effects induced by hypofractionated radiotherapy. Thus, our results suggest that the enhanced apoptosis of NPC cells may be associated with the miR-34a-mediated suppression of c-Myc in a p53-dependent manner.
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Copy and paste a formatted citation
Spandidos Publications style
Long Z, Wang B, Tao D, Huang Y and Tao Z: Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis in human nasopharyngeal carcinoma cells. Int J Mol Med 34: 1388-1394, 2014.
APA
Long, Z., Wang, B., Tao, D., Huang, Y., & Tao, Z. (2014). Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis in human nasopharyngeal carcinoma cells. International Journal of Molecular Medicine, 34, 1388-1394. https://doi.org/10.3892/ijmm.2014.1937
MLA
Long, Z., Wang, B., Tao, D., Huang, Y., Tao, Z."Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis in human nasopharyngeal carcinoma cells". International Journal of Molecular Medicine 34.5 (2014): 1388-1394.
Chicago
Long, Z., Wang, B., Tao, D., Huang, Y., Tao, Z."Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis in human nasopharyngeal carcinoma cells". International Journal of Molecular Medicine 34, no. 5 (2014): 1388-1394. https://doi.org/10.3892/ijmm.2014.1937
Copy and paste a formatted citation
x
Spandidos Publications style
Long Z, Wang B, Tao D, Huang Y and Tao Z: Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis in human nasopharyngeal carcinoma cells. Int J Mol Med 34: 1388-1394, 2014.
APA
Long, Z., Wang, B., Tao, D., Huang, Y., & Tao, Z. (2014). Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis in human nasopharyngeal carcinoma cells. International Journal of Molecular Medicine, 34, 1388-1394. https://doi.org/10.3892/ijmm.2014.1937
MLA
Long, Z., Wang, B., Tao, D., Huang, Y., Tao, Z."Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis in human nasopharyngeal carcinoma cells". International Journal of Molecular Medicine 34.5 (2014): 1388-1394.
Chicago
Long, Z., Wang, B., Tao, D., Huang, Y., Tao, Z."Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis in human nasopharyngeal carcinoma cells". International Journal of Molecular Medicine 34, no. 5 (2014): 1388-1394. https://doi.org/10.3892/ijmm.2014.1937
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