Poly-ε-caprolactone composite scaffolds for bone repair

  • Authors:
    • R. Di Liddo
    • P. Paganin
    • S. Lora
    • D. Dalzoppo
    • C. Giraudo
    • D. Miotto
    • A.  Tasso
    • S.  Barbon
    • M.  Artico
    • E. Bianchi
    • P. P. Parnigotto
    • M. T. Conconi
    • C.  Grandi
  • View Affiliations

  • Published online on: October 1, 2014     https://doi.org/10.3892/ijmm.2014.1954
  • Pages: 1537-1546
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Abstract

Synthetic biomaterials combined with cells and osteogenic factors represent a promising approach for the treatment of a number of orthopedic diseases, such as bone trauma and congenital malformations. To guarantee optimal biological properties, bone substitutes are prepared with a 3D structure and porosity grade functional to drive cell migration and proliferation, diffusion of factors, vascularization and cell waste expulsion. In this study, synthetic hydroxyapatite (HA) or rat bone extracellular matrix (BP) were examined in an effort to optimize the mechanical properties and osteogenic activity of poly-ε-caprolactone scaffolds prepared with alginate threads (PCL-AT). Using rabbit bone marrow-derived mesenchymal stem cells (rMSCs), the effects of PCL composite substrates on cell adhesion, growth and osteogenic differentiation were evaluated. Micro-CT analysis and scanning electron microscopy evidenced that porous PCL scaffolds containing HA or BP acquire a trabecular bone-like structure with interconnected pores homogenously distributed and are characterized by a pore diameter of approximately 10 µm (PCL-AT-BP) or ranging from 10 to 100 µm. Although the porosity grade of both PCL-AT-HA and PCL-AT-BP promoted optimal conditions for the cell growth of rMSCs at the early phase, the presence of BP was crucial to prolong the cell viability at the late phase. Moreover, a precocious expression of Runx2 (at 7 days) was observed in PCL-AT-BP in combination with osteogenic soluble factors suggesting that BP controls better than HA the osteogenic maturation process in bone substitutes.
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December-2014
Volume 34 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Di Liddo R, Paganin P, Lora S, Dalzoppo D, Giraudo C, Miotto D, Tasso A, Barbon S, Artico M, Bianchi E, Bianchi E, et al: Poly-ε-caprolactone composite scaffolds for bone repair. Int J Mol Med 34: 1537-1546, 2014
APA
Di Liddo, R., Paganin, P., Lora, S., Dalzoppo, D., Giraudo, C., Miotto, D. ... Grandi, C. (2014). Poly-ε-caprolactone composite scaffolds for bone repair. International Journal of Molecular Medicine, 34, 1537-1546. https://doi.org/10.3892/ijmm.2014.1954
MLA
Di Liddo, R., Paganin, P., Lora, S., Dalzoppo, D., Giraudo, C., Miotto, D., Tasso, A., Barbon, S., Artico, M., Bianchi, E., Parnigotto, P. P., Conconi, M. T., Grandi, C."Poly-ε-caprolactone composite scaffolds for bone repair". International Journal of Molecular Medicine 34.6 (2014): 1537-1546.
Chicago
Di Liddo, R., Paganin, P., Lora, S., Dalzoppo, D., Giraudo, C., Miotto, D., Tasso, A., Barbon, S., Artico, M., Bianchi, E., Parnigotto, P. P., Conconi, M. T., Grandi, C."Poly-ε-caprolactone composite scaffolds for bone repair". International Journal of Molecular Medicine 34, no. 6 (2014): 1537-1546. https://doi.org/10.3892/ijmm.2014.1954