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Article

Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts

  • Authors:
    • So Young Kim
    • Kyung Chan Go
    • Yi Seop Song
    • Yeon Su Jeong
    • Eun Joo Kim
    • Beom Joon Kim
  • View Affiliations / Copyright

    Affiliations: Major in Biomedical Science, Department of Medicine, Graduate School, Chung-Ang University, Seoul, Republic of Korea, Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Republic of Korea
  • Pages: 1613-1621
    |
    Published online on: October 14, 2014
       https://doi.org/10.3892/ijmm.2014.1969
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Abstract

Skin aging is induced through complex biological processes in human skin caused by proteolysis of collagen and elastin, two structural proteins of the dermal extracellular matrix (ECM). Collagen and elastin degradation can induce the expression of matrix metalloproteinases (MMPs), as well as that of a family of zinc-dependent endopeptidases that play critical roles in skin aging. Moreover, elastase is a metalloproteinase which acts on the degradation of elastin in skin aging, and is also involved in the inhibition or the repair of wrinkle formation. Extract of the mycelium of Tricholoma matsutake (T. matsutake), or pine mushroom, is widely distributed in Asian countries. The extract is from the natural biomaterial of the mushroom which is rich in polysaccharides, including β-glucan. This extract has shown potent bioactive antioxidant, immunomodulatory and antitumoral properties. In the present study, we investigated whether the extract of the mycelium of T. matsutake has effects on elastase activity, as well as on the expression of tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) and MMP-1 under basal conditions. Our results revealed that the extract of the mycelium of T. matsutake significantly decreased elastase activity in a dose-dependent manner and reduced the levels of MMP-1 and MMP-3. On the other hand, the expression of TIMP-1 and tropoelastin was increased in fibroblasts treated with the extract of the mycelium of T. matsutake. However, collagent expression was not affected. In addition, our results demonstrated that the extract of the mycelium of T. matsutake inhibited the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-1 expression and suppressed TPA-induced p38 activity. Therefore, the inhibitory effects of the extract of the mycelium of T. matsutake on MMP-1 induction are mediated by the inhibition of p38 in human fibroblasts. Our data suggest that the extract of the mycelium of T. matsutake may prove to be an effective biomaterial for anti-wrinkle treatment, as it can obstruct the degradation of the dermal ECM.
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Copy and paste a formatted citation
Spandidos Publications style
Kim SY, Go KC, Song YS, Jeong YS, Kim EJ and Kim BJ: Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts. Int J Mol Med 34: 1613-1621, 2014.
APA
Kim, S.Y., Go, K.C., Song, Y.S., Jeong, Y.S., Kim, E.J., & Kim, B.J. (2014). Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts. International Journal of Molecular Medicine, 34, 1613-1621. https://doi.org/10.3892/ijmm.2014.1969
MLA
Kim, S. Y., Go, K. C., Song, Y. S., Jeong, Y. S., Kim, E. J., Kim, B. J."Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts". International Journal of Molecular Medicine 34.6 (2014): 1613-1621.
Chicago
Kim, S. Y., Go, K. C., Song, Y. S., Jeong, Y. S., Kim, E. J., Kim, B. J."Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts". International Journal of Molecular Medicine 34, no. 6 (2014): 1613-1621. https://doi.org/10.3892/ijmm.2014.1969
Copy and paste a formatted citation
x
Spandidos Publications style
Kim SY, Go KC, Song YS, Jeong YS, Kim EJ and Kim BJ: Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts. Int J Mol Med 34: 1613-1621, 2014.
APA
Kim, S.Y., Go, K.C., Song, Y.S., Jeong, Y.S., Kim, E.J., & Kim, B.J. (2014). Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts. International Journal of Molecular Medicine, 34, 1613-1621. https://doi.org/10.3892/ijmm.2014.1969
MLA
Kim, S. Y., Go, K. C., Song, Y. S., Jeong, Y. S., Kim, E. J., Kim, B. J."Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts". International Journal of Molecular Medicine 34.6 (2014): 1613-1621.
Chicago
Kim, S. Y., Go, K. C., Song, Y. S., Jeong, Y. S., Kim, E. J., Kim, B. J."Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts". International Journal of Molecular Medicine 34, no. 6 (2014): 1613-1621. https://doi.org/10.3892/ijmm.2014.1969
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