Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
January-2015 Volume 35 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 35 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): Protective activity of the N‑terminal fragment of PYP1 against acetaminophen‑induced cell death in Chang liver cells

  • Authors:
    • Youn Hee Choi
    • Kenichi Yamaguchi
    • Tatsuya Oda
    • Taek Jeong Nam
  • View Affiliations / Copyright

    Affiliations: Institute of Fisheries Sciences, Pukyong National University, Busan 619‑911, Republic of Korea, Graduate School of Science and Technology, Nagasaki University, Nagasaki 852‑8521, Japan
  • Pages: 271-276
    |
    Published online on: November 5, 2014
       https://doi.org/10.3892/ijmm.2014.1992
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In the present study, the chemical structure and chemoprotective activity of Pyropia yezoensis protein (PYP) were investigated using sodium dodecyl sulfate (SDS)‑polyacrylamide gel electrophoresis, automated protein sequencing, matrix‑assisted laser desorption/ionization‑quadrupole ion trap‑time‑of‑flight mass spectrometry and a chemoprotective assay using a synthetic peptide. The PYP fraction was demonstrated to contain two proteins: PYP1 (10 kDa, SDS‑resistant dimer) and PYP2 (10 kDa). PYP1 is a novel protein showing sequence homology with the hypothetical function‑unknown proteins of Chondrus crispus (Rhodophyta) and Emiliania huxleyi (Haptophyceae). PYP2 is a paralog of an extrinsic protein of photosystem II found in other Rhodophyta. The synthetic peptide PYP1 (1‑20), corresponding to the N‑terminal 20 residues of PYP1 (ALEGGKSSGGGEATRDPEPT), exhibits chemoprotective activity against acetaminophen‑induced cell death in Chang liver cells, indicating that PYP1 is a chemoprotectant of the PYP fraction. A possible association between the structure of PYP and its chemoprotective activity is discussed.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Velasquez-Orta SB, Curtis TP and Logan BE: Energy from algae using microbial fuel cells. Biotechnol Bioeng. 103:1068–1076. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Choi YH, Kim KW, Han HS, Nam TJ and Lee BJ: Dietary Hizikia fusiformis glycoprotein-induced IGF-I and IGFBP-3 associated to somatic growth, polyunsaturated fatty acid metabolism, and immunity in juvenile olive flounder Paralichthys olivaceus. Com Biochem Phys A. 167:1–6. 2014. View Article : Google Scholar

3 

Hirayasu H, Yoshikawa Y, Tsuzuki S and Fushiki T: Sulfated polysaccharides derived from dietary seaweeds increase the esterase activity of a lymphocyte tryptase, granzyme A. J Nutr Sci Vitaminol. 51:475–477. 2005. View Article : Google Scholar

4 

Maeda H, Hosokawa M, Sashima T, Funayama K and Miyashita K: Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochem Biophys Res Commun. 332:392–397. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Yuan YV and Walsh NA: Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food Chem Toxicol. 44:1144–1150. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Hwang HJ, Kim IH and Nam TJ: Effect of a glycoprotein from Hizikia fusiformis on acetaminophen-induced liver injury. Food Chem Toxicol. 46:3475–3481. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Hwang HJ, Kwon MJ, Kim IH and Nam TJ: Chemoprotective effects of a protein from the red algae Porphyra yezoensis on acetaminophen-induced liver injury in rats. Phytother Res. 22:1149–1153. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Go H, Hwang HJ and Nam TJ: A glycoprotein from Laminaria japonica induces apoptosis in HT-29 colon cancer cells. Toxicol In Vitro. 24:1546–1553. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Shin ES, Hwang HJ, Kim IH and Nam TJ: A glycoprotein from Porphyra yezoensis produces anti-inflammatory effects in liposaccharide-stimulated macrophages via the TLR4 signaling pathway. Int J Mol Med. 28:809–815. 2011.PubMed/NCBI

10 

Kim YM, Kim IH and Nam TJ: Induction of apoptosis signaling by glycoprotein of Capsosiphon fulvescens in human gastric cancer (AGS) cells. Nutr Cancer. 64:761–769. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Saga N and Kitade Y: Porphyra: a model plant in marine sciences. Fish Sci. 68(Suppl): 1075–1078. 2002.

12 

Lee EK, Seo SB, Kim TH, et al: Analysis of expressed sequence tags of Porphyra yezoensis. Mol Cells. 10:338–342. 2000.PubMed/NCBI

13 

Nikaido I, Asamizu E, Nakajima M, Nakamura Y, Saga N and Tabata S: Generation of 10,154 expressed sequence tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis. DNA Res. 7:223–227. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Nakamura Y, Sasaki N, Kobayashi M, et al: The first symbiont-free genome sequence of marine red alga, Susabi-nori (Pyropia yezoensis). PLoS One. 8:e571222013. View Article : Google Scholar : PubMed/NCBI

15 

Wang L, Mao Y, Kong F, et al: Complete sequence and analysis of plastid genomes of two economically important red algae: Pyropia haitanensis and Pyropia yezoensis. PLoS One. 8:e659022013. View Article : Google Scholar : PubMed/NCBI

16 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

17 

Weber K and Osborn M: The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 244:4406–4412. 1969.PubMed/NCBI

18 

Yamaguchi K: Preparation and proteomic analysis of chloroplast ribosomes. Chloroplast Research Arabidopsis. Javis RP: 775. Humana Press; New York, NY: pp. 241–264. 2011, View Article : Google Scholar

19 

Larkin MA, Blackshields G, Brown NP, et al: Clustal W and Clustal X version 2.0. Bioinformatics. 23:2947–2948. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Rice P, Longden I and Bleasby A: EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 16:276–277. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Horton P, Park KJ, Obayashi T, et al: WoLF PSORT: protein localization predictor. Nucleic Acids Res. 35(Web Server issue): W585–W587. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Cabra V, Arreguin R, Vazquez-Duhalt R and Farres A: Effect of temperature and pH on the secondary structure and processes of oligomerization of 19 kDa alpha-zein. Biochim Biophys Acta. 1764:110–1118. 2006.

23 

Tatsuno R, Yamaguchi K, Takatani T and Arakawa O: RT-PCR- and MALDI-TOF mass spectrometry-based identification and discrimination of isoforms homologous to pufferfish saxitoxin- and tetrodotoxin-binding protein in the plasma of non-toxic cultured pufferfish (Takifugu rubripes). Biosci Biotechnol Biochem. 77:208–212. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Battaglia M, Olvera-Carrillo Y, Garciarrubio A, Campos F and Covarrubias AA: The enigmatic LEA proteins and other hydrophilins. Plant Physiol. 148:6–24. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Mowla SB, Cuypers A, Driscoll SP, et al: Yeast complementation reveals a role for an Arabidopsis thaliana late embryogenesis abundant (LEA)-like protein in oxidative stress tolerance. Plant J. 48:743–756. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Roose JL, Wegener KM and Pakrasi HB: The extrinsic proteins of Photosystem II. Photosynth Res. 92:369–387. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Nishiyama Y, Los DA and Murata N: PsbU, a protein associated with photosystem II, is required for the acquisition of cellular thermotolerance in Synechococcus species PCC 7002. Plant Physiol. 120:301–308. 1999. View Article : Google Scholar : PubMed/NCBI

28 

Balint I, Bhattacharya J, Perelman A, et al: Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress. FEBS Lett. 580:2117–2122. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Abasova L, Deák Z, Schwarz R and Vass I: The role of the PsbU subunit in the light sensitivity of PSII in the cyanobacterium Synechococcus 7942. J Photochem Photobiol B. 105:149–156. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Adamson GM and Harman AW: Oxidative stress in cultured hepatocytes exposed to acetaminophen. Biochem Pharmacol. 45:2289–2294. 1993. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Choi YH, Yamaguchi K, Oda T and Nam TJ: Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): Protective activity of the N‑terminal fragment of PYP1 against acetaminophen‑induced cell death in Chang liver cells. Int J Mol Med 35: 271-276, 2015.
APA
Choi, Y.H., Yamaguchi, K., Oda, T., & Nam, T.J. (2015). Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): Protective activity of the N‑terminal fragment of PYP1 against acetaminophen‑induced cell death in Chang liver cells. International Journal of Molecular Medicine, 35, 271-276. https://doi.org/10.3892/ijmm.2014.1992
MLA
Choi, Y. H., Yamaguchi, K., Oda, T., Nam, T. J."Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): Protective activity of the N‑terminal fragment of PYP1 against acetaminophen‑induced cell death in Chang liver cells". International Journal of Molecular Medicine 35.1 (2015): 271-276.
Chicago
Choi, Y. H., Yamaguchi, K., Oda, T., Nam, T. J."Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): Protective activity of the N‑terminal fragment of PYP1 against acetaminophen‑induced cell death in Chang liver cells". International Journal of Molecular Medicine 35, no. 1 (2015): 271-276. https://doi.org/10.3892/ijmm.2014.1992
Copy and paste a formatted citation
x
Spandidos Publications style
Choi YH, Yamaguchi K, Oda T and Nam TJ: Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): Protective activity of the N‑terminal fragment of PYP1 against acetaminophen‑induced cell death in Chang liver cells. Int J Mol Med 35: 271-276, 2015.
APA
Choi, Y.H., Yamaguchi, K., Oda, T., & Nam, T.J. (2015). Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): Protective activity of the N‑terminal fragment of PYP1 against acetaminophen‑induced cell death in Chang liver cells. International Journal of Molecular Medicine, 35, 271-276. https://doi.org/10.3892/ijmm.2014.1992
MLA
Choi, Y. H., Yamaguchi, K., Oda, T., Nam, T. J."Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): Protective activity of the N‑terminal fragment of PYP1 against acetaminophen‑induced cell death in Chang liver cells". International Journal of Molecular Medicine 35.1 (2015): 271-276.
Chicago
Choi, Y. H., Yamaguchi, K., Oda, T., Nam, T. J."Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): Protective activity of the N‑terminal fragment of PYP1 against acetaminophen‑induced cell death in Chang liver cells". International Journal of Molecular Medicine 35, no. 1 (2015): 271-276. https://doi.org/10.3892/ijmm.2014.1992
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team