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Article

Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the activation of the STAT3 signaling pathway

  • Authors:
    • Ying Wang
    • Qingzhong Chen
    • Zhuhong Zhang
    • Feng Jiang
    • Xiangda Meng
    • Hua Yan
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China
  • Pages: 471-477
    |
    Published online on: December 11, 2014
       https://doi.org/10.3892/ijmm.2014.2034
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Abstract

Lentivirus vector‑interleukin‑10 green fluorescent protein (LV‑IL‑10‑GFP) was transfected into endothelial progenitor cells (EPCs) in the present study. The aim was to detect the function of IL‑10‑modified EPCs and analyze the molecular mechanism. EPCs were cultured and identified by fluorescent labeling with the von Willebrand factor antibody, vascular endothelial growth factor (VEGF) receptor, Ulex europaeus agglutinin‑1 and acetylated low‑density lipoprotein. Subsequently, EPCs were transfected with LV‑IL‑10‑GFP and lentivirus vector‑noncontain‑GFP as the control group. Enzyme‑linked immunosorbent assay (ELISA) was used to detect the concentrations of cytokines in the supernatant with or without tumor necrosis factor‑α (TNF‑α). All types of cells were assessed by a tube formation assay, adhesion assay and migration assay induced with or without TNF‑α. Cell cycle was assessed by flow cytometry. Western blot analysis was applied to detect the expression of proteins in the cells. ELISA analysis showed that the levels of TNF‑α and IL‑8 in the supernatant without TNF‑α significantly decreased in EPC‑LV‑IL‑10‑GFP (P<0.05 for all). By contrast, the levels of IL‑10 and VEGF were contrasting in association with these. The concentrations of cytokines in the supernatant with TNF‑α were consistent to the supernatant without TNF‑α. There was no statistically significant difference in the average number of EPCs undergoing migration, adhesion, total length and cell growth among the EPC, EPC‑LV‑IL‑10‑GFP and EPC‑LV‑NC‑GFP groups without TNF‑α. Further study showed that EPC‑LV‑IL‑10‑GFP with TNF‑α significantly enhanced EPC migration, adhesion and promoted tube formation (P<0.05 for all). Western blot analysis revealed that the expression of VEGF, matrix metallopeptidase‑9 and phosphorylated‑signal transducer and activator of transcription 3 (p‑STAT3) significantly increased in the EPC‑LV‑IL‑10‑GFP group. Conversely, STAT‑3 expression decreased in the EPC‑LV‑IL‑10‑GFP group. The present study suggested that overexpression of IL‑10 had no effect on migration, adhesion, tubule formation and cell growth of EPCs without TNF‑α. Furthermore, in EPCs stimulated with TNF‑α, the overexpression of IL‑10 improved EPC function, including migration, adhesion and tubule formation by activating the STAT3 signal pathway.
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1 

Huang H, Huang F and Huang JP: Transplantation of bone marrow-derived endothelial progenitor cells overexpressing Delta-like-4 enhances functional neovascularization in ischemic myocardium. Mol Med Rep. 8:1556–1562. 2013.PubMed/NCBI

2 

Barsotti MC, Santoni T, Picoi ME, et al: Endothelial progenitor cell homing in human myocardium in patients with coronary artery disease. Int J Cardiol. 15:516–517. 2014. View Article : Google Scholar

3 

Long J and Wang S, Zhang Y, Liu X, Zhang H and Wang S: The therapeutic effect of vascular endothelial growth factor gene - or heme oxygenase-1 gene-modified endothelialprogenitor cells on neovascularization of rat hindlimb ischemia model. J Vasc Surg. 58:756–765. 2013. View Article : Google Scholar

4 

Roncalli JG, Tongers J, Renault MA and Losordo DW: Endothelial progenitor cells in regenerative medicine and cancer: a decade of research. Trends Biotechnol. 26:276–283. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Grisar J, Aletaha D, Steiner CW, et al: Depletion of endothelial progenitor cells in the peripheral blood of patients with rheumatoid arthritis. Circulation. 111:204–211. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Krishnamurthy P, Lambers E, Verma S, Thorne T, Qin G, Losordo DW and Kishore R: Myocardial knockdown of mRNA-stabilizing protein HuR attenuates post-mi inflammatory response and left ventricular dysfunction in IL-10-null mice. FASEB J. 24:2484–2494. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Krishnamurthy P, Rajasingh J, Lambers E, Qin G, Losordo DW and Kishore R: Il-10 inhibits inflammation and attenuates left ventricular remodeling after myocardial infarction via activation of STAT3 and suppression of HuR. Circ Res. 104:e9–e18. 2009. View Article : Google Scholar :

8 

Yao L, Huang K, Huang D, Wang J, Guo H and Liao Y: Acute myocardial infarction induced increases in plasma tumor necrosis factor-alpha and interleukin-10 are associated with the activation of poly (ADP-ribose) polymerase of circulating mono-nuclear cell. Int J Cardiol. 123:366–368. 2008. View Article : Google Scholar

9 

Jui HY, Lin CH, Hsu WT, et al: Autologous mesenchymal stem cells prevent transplant arteriosclerosis by enhancing local expression of interleukin-10, interferon-gamma, and indoleamine 2,3-dioxygenase. Cell Transplant. 21:971–984. 2012. View Article : Google Scholar

10 

Kawamoto A, Iwasaki H, Kusano K, et al: CD34-positive cells exhibit increased potency and safety for therapeutic neovascularization after myocardial infarction compared with total mononuclear cells. Circulation. 114:2163–2169. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Zhao W, Li JJ, Cao DY, et al: Intravenous injection of mesenchymal stem cells is effective in treating liver fibrosis. World J Gastroenterol. 18:1048–1058. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Krishnamurthy P, Thal M, Verma S, et al: Interleukin-10 deficiency impairs bone marrow-derived endothelial progenitor cell survival and function in ischemic myocardium. Circ Res. 109:1280–1289. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Chen DD, Dong YG, Yuan H and Chen AF: Endothelin 1 activation of endothelin A receptor/NADPH oxidase pathway and diminished antioxidants critically contribute to endothelial progenitor cell reduction and dysfunction in salt-sensitive hypertension. Hypertension. 59:1037–1043. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Marrotte EJ, Chen DD, Hakim JS and Chen AF: Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice. J Clin Invest. 120:4207–4219. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Asahara T, Murohara T, Sullivan A, et al: Isolation of putative progenitor endothelial cells for angiogenesis. Science. 275:964–967. 1997. View Article : Google Scholar : PubMed/NCBI

16 

Peng LH, Tsang SY, Tabata Y and Gao JQ: Genetically-manipulated adult stem cells as therapeutic agents and gene delivery vehicle for wound repair and regeneration. J Control Release. 157:321–330. 2012. View Article : Google Scholar

17 

Golia E, Limongelli G, Natale F, et al: Inflammation and cardiovascular disease: from pathogenesis to therapeutic target. Curr Atheroscler Rep. 16:4352014. View Article : Google Scholar : PubMed/NCBI

18 

Pu X, Wang L, Chang JY, et al: Inflammation-related genetic variants predict toxicity following definitive radiotherapy for lung cancer. Clin Pharmacol Ther. Jul 23–2014.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

19 

Guignabert C and Dorfmuller P: Pathology and pathobiology of pulmonary hypertension. Semin Respir Crit Care Med. 34:551–559. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Inoue T, Croce K, Morooka T, Sakuma M, Node K and Simon DI: Vascular inflammation and repair: implications for re-endothelialization, restenosis, and stent thrombosis. JACC Cardiovasc Interv. 4:1057–1066. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Tang J and Kern TS: Inflammation in diabetic retinopathy. Prog Retin Eye Res. 30:343–358. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Peplow PV: Influence of growth factors and cytokines on angiogenic function of endothelial progenitor cells: a review of in vitro human studies. Growth Factors. 32:83–116. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Palenski TL, Sorenson CM and Sheibani N: Inflammatory cytokine-specific alterations in retinal endothelial cell function. Microvasc Res. 89:57–69. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Lafarge S, Hamzeh-Cognasse H, Chavarin P, Genin C, Garraud O and Cognasse F: A flow cytometry technique to study intracellular signals NF-kappaB and STAT3 in peripheral blood mononuclear cells. BMC Mol Biol. 8:642007. View Article : Google Scholar : PubMed/NCBI

25 

Maher K, Završnik J, Jerič-Kokelj B, Vasiljeva O, Turk B and Kopitar-Jerala N: Decreased IL-10 expression in stefin B-deficient macrophages is regulated by the MAP kinase and STAT-3 signaling pathways. FEBS Lett. 588:720–726. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Leonard WJ and O’Shea JJ: Jaks and STATS: biological implications. Annu Rev Immunol. 16:293–322. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Jin K, Kashiwagi K, Iizuka Y, Tanaka Y, Imai M and Tsukahara S: Matrix metalloproteinases in human diabetic and nondiabetic vitreous. Retina. 21:28–33. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Mazor R, Alsaigh T, Shaked H, et al: Matrix metalloproteinase-1-mediated up-regulation of vascular endothelial growth factor-2 in endothelial cells. J Biol Chem. 288:598–607. 2013. View Article : Google Scholar :

29 

Genís L, Gonzalo P, Tutor AS, et al: Functional interplay between endothelial nitric oxide synthase and membrane type 1-matrix metalloproteinase in migrating endothelial cells. Blood. 110:2916–2923. 2007. View Article : Google Scholar

30 

Shapiro S, Khodalev O, Bitterman H, Auslender R and Lahat N: Different activation forms of MMP-2 oppositely affect the fate of endothelial cells. Am J Physiol Cell Physiol. 298:C942–C951. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Ito TK, Ishii G, Saito S, Yano K, Hoshino A, Suzuki T and Ochiai A: Degradation of soluble vegf receptor-1 by MMP-7 allows VEGF access to endothelial cells. Blood. 113:2363–2369. 2009. View Article : Google Scholar

32 

Huang PH, Chen YH, Wang CH, et al: Matrix metalloproteinase-9 is essential for ischemia-induced neovascularization by modulating bone marrow-derived endothelial progenitor cells. Arterioscler Thromb Vasc Biol. 29:1179–1184. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Chen Q, Zhang Z, Jiang F, Meng X and Yan H: Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the activation of the STAT3 signaling pathway. Int J Mol Med 35: 471-477, 2015.
APA
Wang, Y., Chen, Q., Zhang, Z., Jiang, F., Meng, X., & Yan, H. (2015). Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the activation of the STAT3 signaling pathway. International Journal of Molecular Medicine, 35, 471-477. https://doi.org/10.3892/ijmm.2014.2034
MLA
Wang, Y., Chen, Q., Zhang, Z., Jiang, F., Meng, X., Yan, H."Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the activation of the STAT3 signaling pathway". International Journal of Molecular Medicine 35.2 (2015): 471-477.
Chicago
Wang, Y., Chen, Q., Zhang, Z., Jiang, F., Meng, X., Yan, H."Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the activation of the STAT3 signaling pathway". International Journal of Molecular Medicine 35, no. 2 (2015): 471-477. https://doi.org/10.3892/ijmm.2014.2034
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Chen Q, Zhang Z, Jiang F, Meng X and Yan H: Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the activation of the STAT3 signaling pathway. Int J Mol Med 35: 471-477, 2015.
APA
Wang, Y., Chen, Q., Zhang, Z., Jiang, F., Meng, X., & Yan, H. (2015). Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the activation of the STAT3 signaling pathway. International Journal of Molecular Medicine, 35, 471-477. https://doi.org/10.3892/ijmm.2014.2034
MLA
Wang, Y., Chen, Q., Zhang, Z., Jiang, F., Meng, X., Yan, H."Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the activation of the STAT3 signaling pathway". International Journal of Molecular Medicine 35.2 (2015): 471-477.
Chicago
Wang, Y., Chen, Q., Zhang, Z., Jiang, F., Meng, X., Yan, H."Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the activation of the STAT3 signaling pathway". International Journal of Molecular Medicine 35, no. 2 (2015): 471-477. https://doi.org/10.3892/ijmm.2014.2034
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