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X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging

  • Authors:
    • Quan Qi
    • Chenhui Ding
    • Pingping Hong
    • Gang Yang
    • Yanxin Xie
    • Jing Wang
    • Sunxing Huang
    • Ke He
    • Canquan Zhou
  • View Affiliations / Copyright

    Affiliations: Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 569-578
    |
    Published online on: December 18, 2014
       https://doi.org/10.3892/ijmm.2014.2044
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Abstract

The present study aimed to investigate the X chromosome inactivation (XCI) status in long-term cultured human parthenogenetic embryonic stem cells. One human embryonic stem (hES) cell line and 2 human parthenogenetic embryonic stem (hPES) cell lines were subjected to long-term culture in vitro (>50 passages). Karyotyping, array-based comparative genomic hybridization (aCGH), X-inactive specific transcript (XIST) RNA, immunofluorescence staining and real-time PCR were used to assess the chromosome karyotypes of these cells and the XCI status. X chromosome microdeletion was observed in the hPES-2 cells following culture for 50 passages. As early as 20 passages, XIST RNA expression was detected in the hPES-2 cells and was followed by low X-linked gene expression. The XIST RNA expression level was higher in the differentiated hPES-2 cells. The hPES-2' cells that were subclones of hPES-2 retained the XCI status, and had low XIST and X-linked gene expression. XIST RNA expression remained at a low level in the differentiated hPES-2' cells. The human biparental embryonic stem (hBES)-1 and hPES-1 cells did not exhibit XCI, and the differentiated hPES-1 cells had high expression levels of XIST RNA. In conclusion, the chromosome karyotypes of some hPES cell lines revealed instabilities. Similar to the hES cells, the hPES cells exhibited 3 XCI statuses. The unstable XCI status of the hPES-2 line may have been related to chromosome instability. These unstable chromosomes renedered these cells susceptible to environmental conditions and freezing processes, which may be the result of environmental adaptations.
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Copy and paste a formatted citation
Spandidos Publications style
Qi Q, Ding C, Hong P, Yang G, Xie Y, Wang J, Huang S, He K and Zhou C: X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging. Int J Mol Med 35: 569-578, 2015.
APA
Qi, Q., Ding, C., Hong, P., Yang, G., Xie, Y., Wang, J. ... Zhou, C. (2015). X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging. International Journal of Molecular Medicine, 35, 569-578. https://doi.org/10.3892/ijmm.2014.2044
MLA
Qi, Q., Ding, C., Hong, P., Yang, G., Xie, Y., Wang, J., Huang, S., He, K., Zhou, C."X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging". International Journal of Molecular Medicine 35.3 (2015): 569-578.
Chicago
Qi, Q., Ding, C., Hong, P., Yang, G., Xie, Y., Wang, J., Huang, S., He, K., Zhou, C."X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging". International Journal of Molecular Medicine 35, no. 3 (2015): 569-578. https://doi.org/10.3892/ijmm.2014.2044
Copy and paste a formatted citation
x
Spandidos Publications style
Qi Q, Ding C, Hong P, Yang G, Xie Y, Wang J, Huang S, He K and Zhou C: X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging. Int J Mol Med 35: 569-578, 2015.
APA
Qi, Q., Ding, C., Hong, P., Yang, G., Xie, Y., Wang, J. ... Zhou, C. (2015). X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging. International Journal of Molecular Medicine, 35, 569-578. https://doi.org/10.3892/ijmm.2014.2044
MLA
Qi, Q., Ding, C., Hong, P., Yang, G., Xie, Y., Wang, J., Huang, S., He, K., Zhou, C."X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging". International Journal of Molecular Medicine 35.3 (2015): 569-578.
Chicago
Qi, Q., Ding, C., Hong, P., Yang, G., Xie, Y., Wang, J., Huang, S., He, K., Zhou, C."X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging". International Journal of Molecular Medicine 35, no. 3 (2015): 569-578. https://doi.org/10.3892/ijmm.2014.2044
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