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Article Open Access

MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation

  • Authors:
    • Zhen Yang
    • Jie Hao
    • Zhen‑Ming Hu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 579-586
    |
    Published online on: December 29, 2014
       https://doi.org/10.3892/ijmm.2014.2051
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Abstract

The aim of the present study was to examine the microRNA (miRNA or miR) expression profiles during the chondrogenic differentiation of human adipose‑derived stem cells (hADSCs) and identify the potential mechanisms through which miRNAs may affect the process of chondrogenesis. hADSCs were isolated and cultured. The expression levels of chondrogenic markers was detected by FACS analysis and immunohistochemistry. The miRNA expression profiles were then obtained through a miRNA array and confirmed through northern blot analysis. Putative targets of the miRNAs were predicted and validated through a luciferase reporter assay. The comparison of hADSCs following the induction of chondrogenic differentiation with undifferentiated hADSCs revealed 20 miRNAs that were differentially expressed by at least 2‑fold, and these miRNAs included 12 upregulated miRNAs and 8 downregulated miRNAs. Northern blot analysis further confirmed the miRNA expression levels. Of these miRNAs, the expression of miR‑490‑5p was gradually downregulated following the induction of chondrogenic differentiation. The overexpression of miR‑490‑5p increased the expression of the chondrogenic markers, collagen, type II, alpha 1 (Col2A1), collagen, type X, alpha 1 (Col10A1) and aggrecan. Furthermore, it was confirmed that miR‑490‑5p directly targets bone morphogenetic protein receptor type 2 (BMPR2). In conclusion, in this study, we identified a set of miRNAs that may play key roles in the regulation of the chondrogenic differentiation of hADSCs. Our results may provide a basis for the further investigations into the molecular mechanisms of action of miRNAs in hADSC chondrogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Z, Hao J and Hu ZM: MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation. Int J Mol Med 35: 579-586, 2015.
APA
Yang, Z., Hao, J., & Hu, Z. (2015). MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation. International Journal of Molecular Medicine, 35, 579-586. https://doi.org/10.3892/ijmm.2014.2051
MLA
Yang, Z., Hao, J., Hu, Z."MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation". International Journal of Molecular Medicine 35.3 (2015): 579-586.
Chicago
Yang, Z., Hao, J., Hu, Z."MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation". International Journal of Molecular Medicine 35, no. 3 (2015): 579-586. https://doi.org/10.3892/ijmm.2014.2051
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Z, Hao J and Hu ZM: MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation. Int J Mol Med 35: 579-586, 2015.
APA
Yang, Z., Hao, J., & Hu, Z. (2015). MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation. International Journal of Molecular Medicine, 35, 579-586. https://doi.org/10.3892/ijmm.2014.2051
MLA
Yang, Z., Hao, J., Hu, Z."MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation". International Journal of Molecular Medicine 35.3 (2015): 579-586.
Chicago
Yang, Z., Hao, J., Hu, Z."MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation". International Journal of Molecular Medicine 35, no. 3 (2015): 579-586. https://doi.org/10.3892/ijmm.2014.2051
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