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TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling

  • Authors:
    • Xuyu Zu
    • Jing Zhong
    • Jingjing Tan
    • Li Tan
    • Dong Yang
    • Qinghai Zhang
    • Wenjun Ding
    • Wen Liu
    • Gebo Wen
    • Jianghua Liu
    • Renxian Cao
    • Yuyang Jiang
  • View Affiliations / Copyright

    Affiliations: Institute of Clinical Medicine, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China, Guangdong Provincial Key Laboratory of Chemical Biology, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, P.R. China
    Copyright: © Zu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 693-701
    |
    Published online on: January 5, 2015
       https://doi.org/10.3892/ijmm.2015.2062
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Abstract

Transforming growth factor-β1 (TGF-β1) signaling and high mobility group A (HMGA1) are known to play essential roles in the progression of breast cancer by inducing epithelial-mesenchymal transition. However, the correlation between TGF-β1 and HMGA1 in breast cancer cell is not yet well understood. In this study, we determined the effects of TGF-β1 on HMGA1 expression in breast cancer cells and examined the role of HMGA1 in breast cancer progression. Our results demonstrated that TGF-β1 induced the expression of HMGA1 in both MCF-7 and MDA-MB-231 breast cancer cells, as shown by RT-qPCR and immunofluorescence staining; however, the TGF-β1-induced expression of HMGA was blocked by treatment of the cells with phosphatidylinositol-3 kinase (PI3K) signaling inhibitors. Moreover, the HMGA1 promoter activity was found to be activated by TGF-β1 in the MCF-7 and MDA-MB-231 cells and we found that specificity protein 1 (Sp1) was involved in the TGF-β1-induced HMGA1 promoter activity, as shown by luciferase activity assay. Furthermore, the enforced expression of HMGA1 by transfection with a HMGA1 promoter enhanced cellular oncogenic properties, including proliferation, migration and invasion, and a tissue microarray revealed that breast tumors expressing human epidermal growth factor receptor 2 (HER2) showed higher expression levels of HMGA1 (P=0.007). In addition, higher HMGA1 expression levels were also observed in the ductal breast cancer cases compared with the lobular breast cancer cases (P=0.000). These findings establish the first link between HMGA1 and TGF-β1 in breast cancer, providing further evidence of the pivotal role of HMGA1 in breast cancer progression.
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Copy and paste a formatted citation
Spandidos Publications style
Zu X, Zhong J, Tan J, Tan L, Yang D, Zhang Q, Ding W, Liu W, Wen G, Liu J, Liu J, et al: TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling. Int J Mol Med 35: 693-701, 2015.
APA
Zu, X., Zhong, J., Tan, J., Tan, L., Yang, D., Zhang, Q. ... Jiang, Y. (2015). TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling. International Journal of Molecular Medicine, 35, 693-701. https://doi.org/10.3892/ijmm.2015.2062
MLA
Zu, X., Zhong, J., Tan, J., Tan, L., Yang, D., Zhang, Q., Ding, W., Liu, W., Wen, G., Liu, J., Cao, R., Jiang, Y."TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling". International Journal of Molecular Medicine 35.3 (2015): 693-701.
Chicago
Zu, X., Zhong, J., Tan, J., Tan, L., Yang, D., Zhang, Q., Ding, W., Liu, W., Wen, G., Liu, J., Cao, R., Jiang, Y."TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling". International Journal of Molecular Medicine 35, no. 3 (2015): 693-701. https://doi.org/10.3892/ijmm.2015.2062
Copy and paste a formatted citation
x
Spandidos Publications style
Zu X, Zhong J, Tan J, Tan L, Yang D, Zhang Q, Ding W, Liu W, Wen G, Liu J, Liu J, et al: TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling. Int J Mol Med 35: 693-701, 2015.
APA
Zu, X., Zhong, J., Tan, J., Tan, L., Yang, D., Zhang, Q. ... Jiang, Y. (2015). TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling. International Journal of Molecular Medicine, 35, 693-701. https://doi.org/10.3892/ijmm.2015.2062
MLA
Zu, X., Zhong, J., Tan, J., Tan, L., Yang, D., Zhang, Q., Ding, W., Liu, W., Wen, G., Liu, J., Cao, R., Jiang, Y."TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling". International Journal of Molecular Medicine 35.3 (2015): 693-701.
Chicago
Zu, X., Zhong, J., Tan, J., Tan, L., Yang, D., Zhang, Q., Ding, W., Liu, W., Wen, G., Liu, J., Cao, R., Jiang, Y."TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling". International Journal of Molecular Medicine 35, no. 3 (2015): 693-701. https://doi.org/10.3892/ijmm.2015.2062
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