Open Access

TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling

  • Authors:
    • Xuyu Zu
    • Jing Zhong
    • Jingjing Tan
    • Li Tan
    • Dong Yang
    • Qinghai Zhang
    • Wenjun Ding
    • Wen Liu
    • Gebo Wen
    • Jianghua Liu
    • Renxian Cao
    • Yuyang Jiang
  • View Affiliations

  • Published online on: January 5, 2015     https://doi.org/10.3892/ijmm.2015.2062
  • Pages: 693-701
  • Copyright: © Zu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Transforming growth factor-β1 (TGF-β1) signaling and high mobility group A (HMGA1) are known to play essential roles in the progression of breast cancer by inducing epithelial-mesenchymal transition. However, the correlation between TGF-β1 and HMGA1 in breast cancer cell is not yet well understood. In this study, we determined the effects of TGF-β1 on HMGA1 expression in breast cancer cells and examined the role of HMGA1 in breast cancer progression. Our results demonstrated that TGF-β1 induced the expression of HMGA1 in both MCF-7 and MDA-MB-231 breast cancer cells, as shown by RT-qPCR and immunofluorescence staining; however, the TGF-β1-induced expression of HMGA was blocked by treatment of the cells with phosphatidylinositol-3 kinase (PI3K) signaling inhibitors. Moreover, the HMGA1 promoter activity was found to be activated by TGF-β1 in the MCF-7 and MDA-MB-231 cells and we found that specificity protein 1 (Sp1) was involved in the TGF-β1-induced HMGA1 promoter activity, as shown by luciferase activity assay. Furthermore, the enforced expression of HMGA1 by transfection with a HMGA1 promoter enhanced cellular oncogenic properties, including proliferation, migration and invasion, and a tissue microarray revealed that breast tumors expressing human epidermal growth factor receptor 2 (HER2) showed higher expression levels of HMGA1 (P=0.007). In addition, higher HMGA1 expression levels were also observed in the ductal breast cancer cases compared with the lobular breast cancer cases (P=0.000). These findings establish the first link between HMGA1 and TGF-β1 in breast cancer, providing further evidence of the pivotal role of HMGA1 in breast cancer progression.
View Figures
View References

Related Articles

Journal Cover

March-2015
Volume 35 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zu X, Zhong J, Tan J, Tan L, Yang D, Zhang Q, Ding W, Liu W, Wen G, Liu J, Liu J, et al: TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling. Int J Mol Med 35: 693-701, 2015.
APA
Zu, X., Zhong, J., Tan, J., Tan, L., Yang, D., Zhang, Q. ... Jiang, Y. (2015). TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling. International Journal of Molecular Medicine, 35, 693-701. https://doi.org/10.3892/ijmm.2015.2062
MLA
Zu, X., Zhong, J., Tan, J., Tan, L., Yang, D., Zhang, Q., Ding, W., Liu, W., Wen, G., Liu, J., Cao, R., Jiang, Y."TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling". International Journal of Molecular Medicine 35.3 (2015): 693-701.
Chicago
Zu, X., Zhong, J., Tan, J., Tan, L., Yang, D., Zhang, Q., Ding, W., Liu, W., Wen, G., Liu, J., Cao, R., Jiang, Y."TGF-β1 induces HMGA1 expression in human breast cancer cells: Implications of the involvement of HMGA1 in TGF-β signaling". International Journal of Molecular Medicine 35, no. 3 (2015): 693-701. https://doi.org/10.3892/ijmm.2015.2062