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March-2015 Volume 35 Issue 3

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Article Open Access

Spontaneous immortalization of mouse liver sinusoidal endothelial cells

  • Authors:
    • Xiuhua Zhao
    • Qian Zhao
    • Zhen Luo
    • Yan Yu
    • Na Xiao
    • Xuan Sun
    • Lamei Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shanxi 710004, P.R. China, Department of Adult Stem Cells, Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 617-624
    |
    Published online on: January 13, 2015
       https://doi.org/10.3892/ijmm.2015.2067
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Abstract

The spontaneous immortalization of cells in vitro is a rare event requiring genomic instability, such as alterations in chromosomes and mutations in genes. In the present study, we report a spontaneously immortalized liver sinusoidal endothelial cell (LSEC) line generated from mouse liver. These immortalized LSECs showed typical LSEC characteristics with the structure of transcellular fenestrations, the expression of von Willebrand factor (VWF) and the ability to uptake DiI-acetylated-low density lipoprotein (DiI-Ac-LDL). However, these immortalized LSECs lost the ability to form capillary-like structures, and showed clonal and multilayer growth without contact inhibition. Moreover, their proliferation rate increased with the increase in the number of passages. In addition, these cells obained the expression of CD31 and desmin, and showed an upregulation of p53 protein expression; however, their karyotype was normal, and they could not form colonies in soft agar or tumors in SCID mice. In conclusion, in the present study, we successfully established a spontaneously immortalized LSEC line.
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1 

Wisse E: An electron microscopic study of the fenestrated endothelial lining of rat liver sinusoids. J Ultrastruct Res. 31:125–150. 1970. View Article : Google Scholar : PubMed/NCBI

2 

Braet F, Riches J, Geerts W, Jahn KA, Wisse E and Frederik P: Three-dimensional organization of fenestrae labyrinths in liver sinusoidal endothelial cells. Liver Int. 29:603–613. 2009. View Article : Google Scholar

3 

Elvevold K, Smedsrod B and Martinez I: The liver sinusoidal endothelial cell: a cell type of controversial and confusing identity. Am J Physiol Gastrointest Liver Physiol. 294:G391–G400. 2008. View Article : Google Scholar

4 

Wisse E, De Zanger RB, Charels K, Van Der Smissen P and McCuskey RS: The liver sieve: considerations concerning the structure and function of endothelial fenestrae, the sinusoidal wall and the space of Disse. Hepatology. 5:683–692. 1985. View Article : Google Scholar : PubMed/NCBI

5 

LeCouter J, Moritz DR, Li B, et al: Angiogenesis-independent endothelial protection of liver: role of VEGFR-1. Science. 299:890–893. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Ratsch SB, Gao Q, Srinivasan S, Wazer DE and Band V: Multiple genetic changes are required for efficient immortalization of different subtypes of normal human mammary epithelial cells. Radiat Res. 155:143–150. 2001. View Article : Google Scholar

7 

Yaswen P and Stampfer MR: Molecular changes accompanying senescence and immortalization of cultured human mammary epithelial cells. Int J Biochem Cell Biol. 34:1382–1394. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Tsutsui T, Fujino T, Kodama S, Tainsky MA, Boyd J and Barrett JC: Aflatoxin B1-induced immortalization of cultured skin fibroblasts from a patient with Li-Fraumeni syndrome. Carcinogenesis. 16:25–34. 1995. View Article : Google Scholar : PubMed/NCBI

9 

Todaro GJ and Green H: Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 17:299–313. 1963. View Article : Google Scholar : PubMed/NCBI

10 

Yuspa SH, Poirier MC, Harness JR, Olsom DR and Steinert PM: Specific quantification of mouse and human keratin proteins by radioimmunoassay. Biochem J. 187:281–284. 1980.PubMed/NCBI

11 

Heimann R and Rice RH: Rat esophageal and epidermal keratinocytes: intrinsic differences in culture and derivation of continuous lines. J Cell Physiol. 117:362–367. 1983. View Article : Google Scholar : PubMed/NCBI

12 

Phillips MA and Rice RH: Convergent differentiation in cultured rat cells from nonkeratinized epithelia: keratinocyte character and intrinsic differences. J Cell Biol. 97:686–691. 1983. View Article : Google Scholar : PubMed/NCBI

13 

Davis T and Kipling D: Telomeres and telomerase biology in vertebrates: progress towards a non-human model for replicative senescence and ageing. Biogerontology. 6:371–385. 2005. View Article : Google Scholar

14 

Christman SA, Kong BW, Landry MM, Kim H and Foster DN: Contributions of differential p53 expression in the spontaneous immortalization of a chicken embryo fibroblast cell line. BMC Cell Biol. 7(27): 2006

15 

Dimri G, Band H and Band V: Mammary epithelial cell transformation: insights from cell culture and mouse models. Breast Cancer Res. 7:171–179. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Oh HY, Jin X, Kim JG, et al: Characteristics of primary and immortalized fibroblast cells derived from the miniature and domestic pigs. BMC Cell Biol. 8(20): 2007

17 

Itahana K, Campisi J and Dimri GP: Methods to detect biomarkers of cellular senescence: the senescence-associated beta-galactosidase assay. Methods Mol Biol. 371:21–31. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Gustashaw KM: Chromosome stains. The AGT Cytogenetics Laboratory Manual. Barch MJ, Knutsen T and Spurbeck JL: Lippincott-Raven Press; Philadelphia, New York: pp. 269–280. 1997

19 

Daneker GW, Lund SA, Caughman SW, et al: Culture and characterization of sinusoidal endothelial cells isolated from human liver. In Vitro Cell Dev Biol Anim. 34:370–377. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Karrar A, Broome U, Uzunel M, Qureshi AR and Sumitran-Holgersson S: Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction. Gut. 56:243–252. 2007. View Article : Google Scholar

21 

Knolle PA and Limmer A: Neighborhood politics: the immunoregulatory function of organ-resident liver endothelial cells. Trends Immunol. 22:432–437. 2001. View Article : Google Scholar : PubMed/NCBI

22 

DeLeve LD, Wang X, McCuskey MK and McCuskey RS: Rat liver endothelial cells isolated by anti-CD31 immunomagnetic separation lack fenestrae and sieve plates. Am J Physiol Gastrointest Liver Physiol. 291:G1187–G1189. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Karaa A, Kamoun WS and Clemens MG: Oxidative stress disrupts nitric oxide synthase activation in liver endothelial cells. Free Radic Biol Med. 39:1320–1331. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Knolle PA, Germann T, Treichel U, et al: Endotoxin down-regulates T cell activation by antigen-presenting liver sinusoidal endothelial cells. J Immunol. 162:1401–1407. 1999.PubMed/NCBI

25 

Huang WQ and Wang QR: Bone marrow endothelial cells secrete thymosin beta4 and AcSDKP. Exp Hematol. 29:12–18. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Wang QR, Wang BH, Huang YH, Dai G, Li WM and Yan Q: Purification and growth of endothelial progenitor cells from murine bone marrow mononuclear cells. J Cell Biochem. 103:21–29. 2008. View Article : Google Scholar

27 

DeLeve LD, Wang X, Hu L, McCuskey MK and McCuskey RS: Rat liver sinusoidal endothelial cell phenotype is maintained by paracrine and autocrine regulation. Am J Physiol Gastrointest Liver Physiol. 287:G757–G763. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Braet F and Wisse E: Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review. Comp Hepatol. 1(1): 2002

29 

Namba M, Nishitani K, Fukushima F, Kimoto T and Yuasa Y: Multi-step neoplastic transformation of normal human fibroblasts by Co-60 gamma rays and Ha-ras oncogenes. Mutat Res. 199:415–423. 1988. View Article : Google Scholar : PubMed/NCBI

30 

Chapman S, Liu X, Meyers C, Schlegel R and McBride AA: Human keratinocytes are efficiently immortalized by a Rho kinase inhibitor. J Clin Invest. 120:2619–2626. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Katakura Y, Alam S and Shirahata S: Immortalization by gene transfection. Methods Cell Biol. 57:69–91. 1998. View Article : Google Scholar : PubMed/NCBI

32 

Masamune A, Satoh M, Kikuta K, Suzuki N and Shimosegawa T: Establishment and characterization of a rat pancreatic stellate cell line by spontaneous immortalization. World J Gastroenterol. 9:2751–2758. 2003.PubMed/NCBI

33 

Shay JW, Tomlinson G, Piatyszek MA and Gollahon LS: Spontaneous in vitro immortalization of breast epithelial cells from a patient with Li-Fraumeni syndrome. Mol Cell Biol. 15:425–432. 1995.PubMed/NCBI

34 

Elmore LW, Turner KC, Gollahon LS, Landon MR, Jackson-Cook CK and Holt SE: Telomerase protects cancer-prone human cells from chromosomal instability and spontaneous immortalization. Cancer Biol Ther. 1:391–397. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Zhao C, Meng L, Hu H, et al: Spontaneously immortalised bovine mammary epithelial cells exhibit a distinct gene expression pattern from the breast cancer cells. BMC Cell Biol. 11(82): 2010

36 

Nowak JA, Malowitz J, Girgenrath M, et al: Immortalization of mouse myogenic cells can occur without loss of p16INK4a, p19ARF, or p53 and is accelerated by inactivation of Bax. BMC Cell Biol. 5(1): 2004

37 

Gillio-Meina C, Swan CL, Crellin NK, Stocco DM and Chedrese PJ: Generation of stable cell lines by spontaneous immortalization of primary cultures of porcine granulosa cells. Mol Reprod Dev. 57:366–374. 2000. View Article : Google Scholar : PubMed/NCBI

38 

Denhardt DT, Edwards DR, McLeod M, Norton G, Parfett CL and Zimmer M: Spontaneous immortalization of mouse embryo cells: strain differences and changes in gene expression with particular reference to retroviral gag-pol genes. Exp Cell Res. 192:128–136. 1991. View Article : Google Scholar : PubMed/NCBI

39 

Li WM, Huang YH, Jiang DZ and Wang QR: The effects of cytokines produced by bone marrow endothelial cells on the regulation of hematopoiesis. Sheng Li Xue Bao. 52:45–49. 2000.(In Chinese).

40 

Duan HX, Cheng LM, Wang J, Hu LS and Lu GX: Angiogenic potential difference between two types of endothelial progenitor cells from human umbilical cord blood. Cell Biol Int. 30:1018–1027. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao X, Zhao Q, Luo Z, Yu Y, Xiao N, Sun X and Cheng L: Spontaneous immortalization of mouse liver sinusoidal endothelial cells. Int J Mol Med 35: 617-624, 2015.
APA
Zhao, X., Zhao, Q., Luo, Z., Yu, Y., Xiao, N., Sun, X., & Cheng, L. (2015). Spontaneous immortalization of mouse liver sinusoidal endothelial cells. International Journal of Molecular Medicine, 35, 617-624. https://doi.org/10.3892/ijmm.2015.2067
MLA
Zhao, X., Zhao, Q., Luo, Z., Yu, Y., Xiao, N., Sun, X., Cheng, L."Spontaneous immortalization of mouse liver sinusoidal endothelial cells". International Journal of Molecular Medicine 35.3 (2015): 617-624.
Chicago
Zhao, X., Zhao, Q., Luo, Z., Yu, Y., Xiao, N., Sun, X., Cheng, L."Spontaneous immortalization of mouse liver sinusoidal endothelial cells". International Journal of Molecular Medicine 35, no. 3 (2015): 617-624. https://doi.org/10.3892/ijmm.2015.2067
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao X, Zhao Q, Luo Z, Yu Y, Xiao N, Sun X and Cheng L: Spontaneous immortalization of mouse liver sinusoidal endothelial cells. Int J Mol Med 35: 617-624, 2015.
APA
Zhao, X., Zhao, Q., Luo, Z., Yu, Y., Xiao, N., Sun, X., & Cheng, L. (2015). Spontaneous immortalization of mouse liver sinusoidal endothelial cells. International Journal of Molecular Medicine, 35, 617-624. https://doi.org/10.3892/ijmm.2015.2067
MLA
Zhao, X., Zhao, Q., Luo, Z., Yu, Y., Xiao, N., Sun, X., Cheng, L."Spontaneous immortalization of mouse liver sinusoidal endothelial cells". International Journal of Molecular Medicine 35.3 (2015): 617-624.
Chicago
Zhao, X., Zhao, Q., Luo, Z., Yu, Y., Xiao, N., Sun, X., Cheng, L."Spontaneous immortalization of mouse liver sinusoidal endothelial cells". International Journal of Molecular Medicine 35, no. 3 (2015): 617-624. https://doi.org/10.3892/ijmm.2015.2067
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