Open Access

Astragaloside IV ameliorates acute pancreatitis in rats by inhibiting the activation of nuclear factor-κB

  • Authors:
    • Lei Qiu
    • Guojian Yin
    • Li Cheng
    • Yuting Fan
    • Wenqin Xiao
    • Ge Yu
    • Miao Xing
    • Rongrong Jia
    • Ruiqing Sun
    • Xiuying Ma
    • Guoyong Hu
    • Xingpeng Wang
    • Maochun Tang
    • Yan Zhao
  • View Affiliations

  • Published online on: January 16, 2015     https://doi.org/10.3892/ijmm.2015.2070
  • Pages: 625-636
  • Copyright: © Qiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

This study aimed to investigate the effects of astragaloside IV (AS-IV; 3-O-β-D-xylopyranosyl-6-O‑β-D-glucopyranosylcycloastragenol), which has been reported to have comprehensive pharmacological functions, on sodium taurocholate (NaTc)/L-arginine (L-Arg)-induced acute pancreatitis (AP) in rats in vivo and in rat pancreatic acinar cells in vitro. NaTc-induced experimental AP was induced in rats by injecting 4% NaTc (0.1 ml/100 g) in the retrograde direction of the biliopancreatic duct. L-Arg-induced experimental AP was induced in rats by 2 intraperitoneal injections of 20% L-arg (3 g/kg), with an interval of 1 h between the injections. The rats were pre-treated AS-IV (50 mg/kg) or the vehicle (DMSO) 2 h prior to the induction of AP. Enzyme-linked immunosorbent assay, H&E staining, myeloperoxidase (MPO) activity, reverse transcription-quantitative PCR, western blot analysis and immunohistochemistry were used to evaluate the effects of AS-IV on AP. The results revealed that treatment with AS-IV significantly reduced serum amylase and lipase levels, pancreatic pathological alterations, the secretion of pro-inflammatory cytokines, MPO activity, and the protein expression of nuclear factor-κB (NF-κB) in vivo. Moreover, pre-treatment with AS-IV significantly increased the expression levels of manganese superoxide dismutase and cuprum/zinc superoxide dismutase. In the in vitro experiment, treatment of the cells with AS-IV aslo reduced rat pancreatic acinar cell necrosis and nuclear NF-κB activity, and enhanced the protein expression of superoxide dismutase. In conclusion, this study indicates that the protective effects of AS-IV on experimental AP in rats may be closely related to the inhibition of NF-κB. In addition, our results indicate that AS-IV may exert potential antioxidant effects on AP. Therefore, AS-IV may be an effective therapeutic agent for AP.
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March-2015
Volume 35 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Qiu L, Yin G, Cheng L, Fan Y, Xiao W, Yu G, Xing M, Jia R, Sun R, Ma X, Ma X, et al: Astragaloside IV ameliorates acute pancreatitis in rats by inhibiting the activation of nuclear factor-κB. Int J Mol Med 35: 625-636, 2015
APA
Qiu, L., Yin, G., Cheng, L., Fan, Y., Xiao, W., Yu, G. ... Zhao, Y. (2015). Astragaloside IV ameliorates acute pancreatitis in rats by inhibiting the activation of nuclear factor-κB. International Journal of Molecular Medicine, 35, 625-636. https://doi.org/10.3892/ijmm.2015.2070
MLA
Qiu, L., Yin, G., Cheng, L., Fan, Y., Xiao, W., Yu, G., Xing, M., Jia, R., Sun, R., Ma, X., Hu, G., Wang, X., Tang, M., Zhao, Y."Astragaloside IV ameliorates acute pancreatitis in rats by inhibiting the activation of nuclear factor-κB". International Journal of Molecular Medicine 35.3 (2015): 625-636.
Chicago
Qiu, L., Yin, G., Cheng, L., Fan, Y., Xiao, W., Yu, G., Xing, M., Jia, R., Sun, R., Ma, X., Hu, G., Wang, X., Tang, M., Zhao, Y."Astragaloside IV ameliorates acute pancreatitis in rats by inhibiting the activation of nuclear factor-κB". International Journal of Molecular Medicine 35, no. 3 (2015): 625-636. https://doi.org/10.3892/ijmm.2015.2070