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Article

Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro

  • Authors:
    • Le Yang
    • Jin Hu
    • Hong-Zhen Hao
    • Zhao Yin
    • Gang Liu
    • Xiao-Jing Zou
  • View Affiliations / Copyright

    Affiliations: Department of Emergency Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Otolaryngology, Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Anesthesiology and Critical Care Medicine, Laboratory of Anesthesiology and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 1026-1032
    |
    Published online on: February 2, 2015
       https://doi.org/10.3892/ijmm.2015.2087
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Abstract

The differentiation of atrial fibroblasts into myofibroblasts is a critical event in atrial fibrosis. One of the most important factors in atrial fibroblast differentiation is transforming growth factor-β1 (TGF-β1). Accumulating evidence indicates that sodium tanshinone IIA sulfonate (STS) possesses antifibrotic properties. In this study, we therefore investigated whether STS attenuates the TGF-β1‑induced differentiation of atrial fibroblasts. TGF-β1 enhanced collagen production, collagen synthesis and the expression of collagen type I and III, as shown by hydroxyproline assay, collagen synthesis assay and western blot analysis, respectively. In addition, as shown by immunohistochemistry and western blot analysis, TGF-β1 enhanced the expression of α-smooth muscle actin (α-SMA), which is the hallmark of myofibroblast differentiation. These responses were attenuated by treatment with STS. In addition, STS suppressed the TGF-β1‑induced expression of phosphorylated (p)Smad/pSmad3 expression and nuclear translocation. Furthermore, STS supressed extracellular signal-regulated kinase (ERK) phosphorylation. In conclusion, the current study demonstrates that STS exerts antifibrotic effects by modulating atrial fibroblast differentiation through ERK phosphorylation and the Smad pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Hu J, Hao H, Yin Z, Liu G and Zou X: Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro. Int J Mol Med 35: 1026-1032, 2015.
APA
Yang, L., Hu, J., Hao, H., Yin, Z., Liu, G., & Zou, X. (2015). Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro. International Journal of Molecular Medicine, 35, 1026-1032. https://doi.org/10.3892/ijmm.2015.2087
MLA
Yang, L., Hu, J., Hao, H., Yin, Z., Liu, G., Zou, X."Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro". International Journal of Molecular Medicine 35.4 (2015): 1026-1032.
Chicago
Yang, L., Hu, J., Hao, H., Yin, Z., Liu, G., Zou, X."Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro". International Journal of Molecular Medicine 35, no. 4 (2015): 1026-1032. https://doi.org/10.3892/ijmm.2015.2087
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Hu J, Hao H, Yin Z, Liu G and Zou X: Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro. Int J Mol Med 35: 1026-1032, 2015.
APA
Yang, L., Hu, J., Hao, H., Yin, Z., Liu, G., & Zou, X. (2015). Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro. International Journal of Molecular Medicine, 35, 1026-1032. https://doi.org/10.3892/ijmm.2015.2087
MLA
Yang, L., Hu, J., Hao, H., Yin, Z., Liu, G., Zou, X."Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro". International Journal of Molecular Medicine 35.4 (2015): 1026-1032.
Chicago
Yang, L., Hu, J., Hao, H., Yin, Z., Liu, G., Zou, X."Sodium tanshinone IIA sulfonate attenuates the transforming growth factor-β1-induced differentiation of atrial fibroblasts into myofibroblasts in vitro". International Journal of Molecular Medicine 35, no. 4 (2015): 1026-1032. https://doi.org/10.3892/ijmm.2015.2087
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