Saikosaponin a, an active compound of Radix Bupleuri, attenuates inflammation in hypertrophied 3T3-L1 adipocytes via ERK/NF-κB signaling pathways

  • Authors:
    • Sung Ok Kim
    • Ji Yeoung Park
    • Seo Young Jeon
    • Chea Ha Yang
    • Mi Ryeo Kim
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  • Published online on: February 6, 2015     https://doi.org/10.3892/ijmm.2015.2093
  • Pages: 1126-1132
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Abstract

Bupleurum falcatum L. is employed in oriental medicine in Korea. This root has been used for anti‑inflammatory, anti‑pyretic, and anti‑hepatotoxic effects in the treatments of common cold, fever, and hepatitis. One of major bioactive compounds of Radix Bupleuri is the saikosaponin a (SSNa). However, little is known concerning the effects of SSNa on obesity associated with a state of low‑grade inflammation. Consequently, this study was conducted to determine the inhibition of the inflammation pathway of SSNa in obesity. MTT assay was conducted for cytotoxicity and viability; nuclear and cytoplasmic fractions were extracted from adipocytes for translocation of nuclear factor‑κB cells (NF‑κB); nitric oxide (NO) production and secretion using Griess reagent; reverse transcription‑polymerase chain reaction (RT‑PCR) and immunoblotting for mRNA and protein levels associated with inflammation in the hypertrophied adipocytes. The results revealed that SSNa significantly decreased the expression of tumor necrosis factor‑α (TNFα), interleukin (IL)‑1β and IL‑6 as proinflammatory cytokines, compared to that of non‑treated control cells. Inducible nitric oxide synthase (iNOS) and cyclooxygenase‑2 (COX‑2) as inflammatory factors were reduced by treatment of these cells with SSNa and also suppressed NO production. Phosphorylation of IκBα was inhibited and translocation of NF‑κB was suppressed via the ERK pathway in response to SSNa treatment. In conclusion, the results demonstrated that SSNa can inhibit the expression of inflammatory‑associatied genes in hypertrophied 3T3‑L1 adipocytes and is a potent inhibitor of NF‑κB activation. Thus these results suggest that SSNa is a novel therapeutic agent against that can be used against obesity‑associated inflammation.
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April-2015
Volume 35 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim SO, Park JY, Jeon SY, Yang CH and Kim MR: Saikosaponin a, an active compound of Radix Bupleuri, attenuates inflammation in hypertrophied 3T3-L1 adipocytes via ERK/NF-κB signaling pathways. Int J Mol Med 35: 1126-1132, 2015.
APA
Kim, S.O., Park, J.Y., Jeon, S.Y., Yang, C.H., & Kim, M.R. (2015). Saikosaponin a, an active compound of Radix Bupleuri, attenuates inflammation in hypertrophied 3T3-L1 adipocytes via ERK/NF-κB signaling pathways. International Journal of Molecular Medicine, 35, 1126-1132. https://doi.org/10.3892/ijmm.2015.2093
MLA
Kim, S. O., Park, J. Y., Jeon, S. Y., Yang, C. H., Kim, M. R."Saikosaponin a, an active compound of Radix Bupleuri, attenuates inflammation in hypertrophied 3T3-L1 adipocytes via ERK/NF-κB signaling pathways". International Journal of Molecular Medicine 35.4 (2015): 1126-1132.
Chicago
Kim, S. O., Park, J. Y., Jeon, S. Y., Yang, C. H., Kim, M. R."Saikosaponin a, an active compound of Radix Bupleuri, attenuates inflammation in hypertrophied 3T3-L1 adipocytes via ERK/NF-κB signaling pathways". International Journal of Molecular Medicine 35, no. 4 (2015): 1126-1132. https://doi.org/10.3892/ijmm.2015.2093