Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling

  • Authors:
    • Ji-Won Park
    • Ok-Kyoung Kwon
    • Jung-Hee Kim
    • Sei-Ryang Oh
    • Jae-Hong Kim
    • Jin-Hyub Paik
    • Bambang Marwoto
    • Rifatul Widjhati
    • Fifit Juniarti
    • Doddy Irawan
    • Kyung-Seop Ahn
  • View Affiliations

  • Published online on: February 25, 2015     https://doi.org/10.3892/ijmm.2015.2107
  • Pages: 987-994
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Abstract

Rhododendron album Blume (RA) has traditionally been used as an herbal medicine and is considered to have anti-inflammatory properties. In the present study, we screened RA extracts with anti-inflammatory properties. The biological effects of an RA methanol extract (RAME) on inflammation were investigated in lipopolysaccharide (LPS)-stimulated mouse RAW264.7 cells. We investigated the effects of RAME on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW264.7 cells. To explore the anti-inflammatory mechanisms of RAME, we measured the mRNA and protein expression of pro-inflammatory mediators induced by RAME in the LPS-stimulated RAW264.7 cells by RT-PCR and western blot analysis, respectively. RAME significantly inhibited the production of NO, PGE2, interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α in the LPS-stimulated RAW264.7 cells. It also suppressed the mRNA and protein expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and mitogen-activated protein kinases (MAPKs) with a concomitant decrease in the nuclear translocation of nuclear factor-κB (NF-κB) in the LPS-stimulated RAW264.7 cells. These results indicate that RAME inhibits LPS-induced inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-κB activation. We therefore suggest that RAME may be prove to be an effective therapeutic agent for the treatment of inflammatory diseases.
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April-2015
Volume 35 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Park J, Kwon O, Kim J, Oh S, Kim J, Paik J, Marwoto B, Widjhati R, Juniarti F, Irawan D, Irawan D, et al: Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling. Int J Mol Med 35: 987-994, 2015
APA
Park, J., Kwon, O., Kim, J., Oh, S., Kim, J., Paik, J. ... Ahn, K. (2015). Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling. International Journal of Molecular Medicine, 35, 987-994. https://doi.org/10.3892/ijmm.2015.2107
MLA
Park, J., Kwon, O., Kim, J., Oh, S., Kim, J., Paik, J., Marwoto, B., Widjhati, R., Juniarti, F., Irawan, D., Ahn, K."Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling". International Journal of Molecular Medicine 35.4 (2015): 987-994.
Chicago
Park, J., Kwon, O., Kim, J., Oh, S., Kim, J., Paik, J., Marwoto, B., Widjhati, R., Juniarti, F., Irawan, D., Ahn, K."Rhododendron album Blume inhibits iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells through the downregulation of NF-κB signaling". International Journal of Molecular Medicine 35, no. 4 (2015): 987-994. https://doi.org/10.3892/ijmm.2015.2107