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Article

20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes

  • Authors:
    • Tae-Gyu Lim
    • Ae Ji Jeon
    • Ji Hye Yoon
    • Dasom Song
    • Jong-Eun Kim
    • Jung Yeon Kwon
    • Jong Rhan Kim
    • Nam Joo Kang
    • Jun-Seong Park
    • Myeong Hun Yeom
    • Deok-Kun Oh
    • Yoongho Lim
    • Charles C. Lee
    • Chang Yong Lee
    • Ki Won Lee
  • View Affiliations / Copyright

    Affiliations: Advanced Institutes of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea, Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea, WCU Biomodulation Major, Department of Agricultural Biotechnology and Institute on Aging, Seoul National University, Seoul 151-921, Republic of Korea, School of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea, Skin Research Institute, Amorepacific Corporation R&D Center, Yongin 341-1, Republic of Korea, Department of Food Science, Cornell University, Ithaca, NY 14850, USA
  • Pages: 1388-1394
    |
    Published online on: March 2, 2015
       https://doi.org/10.3892/ijmm.2015.2121
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Abstract

The aim of the present study was to determine the mechanisms through which 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol (20GPPD) promotes the production of hyaluronic acid (HA) in human keratinocytes. 20GPPD is the primary bioactive metabolite of Rb1, a major ginsenoside found in ginseng (Panax ginseng). We sought to elucidate the underlying mechanisms behind the 20GPPD-induced production of HA. We found that 20GPPD induced an increase in HA production by elevating hyaluronan synthase 2 (HAS2) expression in human keratinocytes. The phosphorylation of extracellular signal-regulated kinase (ERK) and Akt was also enhanced by 20GPPD in a dose-dependent manner. The pharmacological inhibition of ERK (using U0126) or Akt (using LY294002) suppressed the 20GPPD-induced expression of HAS2, whereas treatment with an epidermal growth factor receptor (EGFR) inhibitor (AG1478) or an intracellular Ca2+ chelator (BAPTA/AM) did not exert any observable effects. The increased Src phosphorylation was also confirmed following treatment with 20GPPD in the human keratinocytes. Following pre-treatment with the Src inhibitor, PP2, both HA production and HAS2 expression were attenuated. Furthermore, the 20GPPD-enhanced ERK and Akt signaling decreased following treatment with PP2. Taken together, our results suggest that Src kinase plays a critical role in the 20GPPD-induced production of HA by acting as an upstream modulator of ERK and Akt activity in human keratinocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Lim T, Jeon AJ, Yoon JH, Song D, Kim J, Kwon JY, Kim JR, Kang NJ, Park J, Yeom MH, Yeom MH, et al: 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes. Int J Mol Med 35: 1388-1394, 2015.
APA
Lim, T., Jeon, A.J., Yoon, J.H., Song, D., Kim, J., Kwon, J.Y. ... Lee, K.W. (2015). 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes. International Journal of Molecular Medicine, 35, 1388-1394. https://doi.org/10.3892/ijmm.2015.2121
MLA
Lim, T., Jeon, A. J., Yoon, J. H., Song, D., Kim, J., Kwon, J. Y., Kim, J. R., Kang, N. J., Park, J., Yeom, M. H., Oh, D., Lim, Y., Lee, C. C., Lee, C. Y., Lee, K. W."20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes". International Journal of Molecular Medicine 35.5 (2015): 1388-1394.
Chicago
Lim, T., Jeon, A. J., Yoon, J. H., Song, D., Kim, J., Kwon, J. Y., Kim, J. R., Kang, N. J., Park, J., Yeom, M. H., Oh, D., Lim, Y., Lee, C. C., Lee, C. Y., Lee, K. W."20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes". International Journal of Molecular Medicine 35, no. 5 (2015): 1388-1394. https://doi.org/10.3892/ijmm.2015.2121
Copy and paste a formatted citation
x
Spandidos Publications style
Lim T, Jeon AJ, Yoon JH, Song D, Kim J, Kwon JY, Kim JR, Kang NJ, Park J, Yeom MH, Yeom MH, et al: 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes. Int J Mol Med 35: 1388-1394, 2015.
APA
Lim, T., Jeon, A.J., Yoon, J.H., Song, D., Kim, J., Kwon, J.Y. ... Lee, K.W. (2015). 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes. International Journal of Molecular Medicine, 35, 1388-1394. https://doi.org/10.3892/ijmm.2015.2121
MLA
Lim, T., Jeon, A. J., Yoon, J. H., Song, D., Kim, J., Kwon, J. Y., Kim, J. R., Kang, N. J., Park, J., Yeom, M. H., Oh, D., Lim, Y., Lee, C. C., Lee, C. Y., Lee, K. W."20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes". International Journal of Molecular Medicine 35.5 (2015): 1388-1394.
Chicago
Lim, T., Jeon, A. J., Yoon, J. H., Song, D., Kim, J., Kwon, J. Y., Kim, J. R., Kang, N. J., Park, J., Yeom, M. H., Oh, D., Lim, Y., Lee, C. C., Lee, C. Y., Lee, K. W."20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginsenoside Rb1, enhances the production of hyaluronic acid through the activation of ERK and Akt mediated by Src tyrosin kinase in human keratinocytes". International Journal of Molecular Medicine 35, no. 5 (2015): 1388-1394. https://doi.org/10.3892/ijmm.2015.2121
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