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International Journal of Molecular Medicine
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Article

IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen

  • Authors:
    • Xianchen Huang
    • Yiqi Jin
    • Dayong Zhou
    • Guoxiong Xu
    • Jian Huang
    • Liming Shen
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu 215002, P.R. China
  • Pages: 1460-1466
    |
    Published online on: March 12, 2015
       https://doi.org/10.3892/ijmm.2015.2134
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Abstract

Vascular smooth muscle cell (VSMC) proliferation and migration has been proven to be a critical event in the development of varicosity. Variations in estrogen levels, a pathological event related to age and pregnancy, play a role in the pathogenesis of varicosity. Previous studies have reported a different response of VSMCs following estrogen stimulation. However, the exact mechanisms involved have not yet been elucidated. In the present study, we examined the responses of lesion and normal VSMCs treated with 10-8 M 17β-estradiol (E2) for 24 h. A differential effect of exposure to E2 was observed in these cells. IQ-domain GTPase-activating protein 1 (IQGAP1), a scaffold protein, was overexpressed in the lesion VSMCs and was shown to modulate VSMC proliferation and migration in response to E2. Furthermore, the increased expression of IQGAP1 was found to be intimately associated with a high activity of estrogen receptor α (ERα), which has been implicated in the regulation of VSMC physiological function. Additionally, we found that two critical kinases, Akt and extracellular signal-regulated kinase (ERK), mediated the activation of ERα and VSMC proliferation. According to our results, we thus concluded that high levels of IQGAP1 in VSMCs regulate the physiological reaction of the cells in response to estrogen exposure, and that kinases are involved in the process by mediating ERα activation. In view of the essential role of IQGAP1 in the physiological function of VSMCs, targeting this molecule may prove to be a promising strategy for the treatment of varicosity.
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Copy and paste a formatted citation
Spandidos Publications style
Huang X, Jin Y, Zhou D, Xu G, Huang J and Shen L: IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen. Int J Mol Med 35: 1460-1466, 2015.
APA
Huang, X., Jin, Y., Zhou, D., Xu, G., Huang, J., & Shen, L. (2015). IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen. International Journal of Molecular Medicine, 35, 1460-1466. https://doi.org/10.3892/ijmm.2015.2134
MLA
Huang, X., Jin, Y., Zhou, D., Xu, G., Huang, J., Shen, L."IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen". International Journal of Molecular Medicine 35.5 (2015): 1460-1466.
Chicago
Huang, X., Jin, Y., Zhou, D., Xu, G., Huang, J., Shen, L."IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen". International Journal of Molecular Medicine 35, no. 5 (2015): 1460-1466. https://doi.org/10.3892/ijmm.2015.2134
Copy and paste a formatted citation
x
Spandidos Publications style
Huang X, Jin Y, Zhou D, Xu G, Huang J and Shen L: IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen. Int J Mol Med 35: 1460-1466, 2015.
APA
Huang, X., Jin, Y., Zhou, D., Xu, G., Huang, J., & Shen, L. (2015). IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen. International Journal of Molecular Medicine, 35, 1460-1466. https://doi.org/10.3892/ijmm.2015.2134
MLA
Huang, X., Jin, Y., Zhou, D., Xu, G., Huang, J., Shen, L."IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen". International Journal of Molecular Medicine 35.5 (2015): 1460-1466.
Chicago
Huang, X., Jin, Y., Zhou, D., Xu, G., Huang, J., Shen, L."IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen". International Journal of Molecular Medicine 35, no. 5 (2015): 1460-1466. https://doi.org/10.3892/ijmm.2015.2134
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