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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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June-2015 Volume 35 Issue 6

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Article

Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression

  • Authors:
    • Yanhong Zhou
    • Zhaohui Hu
    • Ningning Li
    • Renjie Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, The People's Hospital of Liuzhou, Liuzhou 545006, P.R. China, Department of Spine Surgery, The People's Hospital of Liuzhou, Liuzhou 545006, P.R. China
  • Pages: 1729-1733
    |
    Published online on: March 31, 2015
       https://doi.org/10.3892/ijmm.2015.2159
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Abstract

As a pro-inflammatory cytokine, interleukin-32 (IL-32) is reported to play an important role in tumor development and progression. However, its effects on the invasion and motility of osteosarcoma cells remain elusive. The aim of the present study was to determine the molecular mechanisms of IL-32 in osteosarcoma cells using RT-PCR and western blot analysis. The results showed that IL-32 stimulation dose-dependently promoted the invasion and motility of osteosarcoma cells. Knockdown of endogenous IL-32 by siRNA inhibited osteosarcoma cell invasion and motility. Moreover, IL-32 induced the activation of AKT in a time‑dependent manner. IL-32 stimulation was also capable of increasing the expression and secretion of matrix metalloproteinase (MMP)-13, which is involved in tumor invasion and metastasis. In addition, blockade of AKT activation suppressed IL-32-mediated invasion, motility and MMP-13 upregulation in osteosarcoma cells. Taken together, our results suggest that IL-32 stimulation promotes the invasion and motility of osteosarcoma cells, possibly via the activation of AKT and the upregulation of MMP-13 expression. Thus, IL-32 may serve as a marker for diagnosis, as well as for the treatment of osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Hu Z, Li N and Jiang R: Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression. Int J Mol Med 35: 1729-1733, 2015.
APA
Zhou, Y., Hu, Z., Li, N., & Jiang, R. (2015). Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression. International Journal of Molecular Medicine, 35, 1729-1733. https://doi.org/10.3892/ijmm.2015.2159
MLA
Zhou, Y., Hu, Z., Li, N., Jiang, R."Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression". International Journal of Molecular Medicine 35.6 (2015): 1729-1733.
Chicago
Zhou, Y., Hu, Z., Li, N., Jiang, R."Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression". International Journal of Molecular Medicine 35, no. 6 (2015): 1729-1733. https://doi.org/10.3892/ijmm.2015.2159
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Hu Z, Li N and Jiang R: Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression. Int J Mol Med 35: 1729-1733, 2015.
APA
Zhou, Y., Hu, Z., Li, N., & Jiang, R. (2015). Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression. International Journal of Molecular Medicine, 35, 1729-1733. https://doi.org/10.3892/ijmm.2015.2159
MLA
Zhou, Y., Hu, Z., Li, N., Jiang, R."Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression". International Journal of Molecular Medicine 35.6 (2015): 1729-1733.
Chicago
Zhou, Y., Hu, Z., Li, N., Jiang, R."Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression". International Journal of Molecular Medicine 35, no. 6 (2015): 1729-1733. https://doi.org/10.3892/ijmm.2015.2159
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