NLRP3 inflammasome is responsible for Hantavirus inducing interleukin-1β in THP-1 cells Corrigendum in /10.3892/ijmm.2019.4155

Ye,Wei; Lei,Yingfeng; Yu,Mengmeng; Xu,Yongni; Cao,Mengyuan; Yu,Lan; Zhang,Liang; Li,Puyuan; Bai,Wentao; Xu,Zhikai; Zhang,Fanglin

doi:10.3892/ijmm.2015.2162

NLRP3 inflammasome is responsible for Hantavirus inducing interleukin-1β in THP-1 cells

Corrigendum in: /10.3892/ijmm.2019.4155

Authors:
- Wei Ye
- Yingfeng Lei
- Mengmeng Yu
- Yongni Xu
- Mengyuan Cao
- Lan Yu
- Liang Zhang
- Puyuan Li
- Wentao Bai
- Zhikai Xu
- Fanglin Zhang
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Affiliations: Department of Microbiology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Health Drug and Instrument Control, General Logistics Department of the Ministry, Beijing 100071, P.R. China, Department of Minimally Invasive Surgery, General Surgery Center, General Hospital of Chengdu Military Region, Chengdu, Sichuan 610083, P.R. China
Published online on: April 1, 2015 https://doi.org/10.3892/ijmm.2015.2162
Pages: 1633-1640

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Abstract

Persistent high fever is one typical clinical symptom of hemorrhagic fever with renal syndrome (HFRS) and circulating interleukin‑1β (IL‑1β) is elevated throughout HFRS. The mechanisms responsible for viral induction of IL‑1β secretion are unknown. In the present study, Hantaan virus (HTNV) induced the secretion of IL‑1β in the human monocytic cell line THP‑1. Induction of IL‑1β by HTNV relies on the activation of caspase‑1. Small hairpin RNA knockdown in HTNV‑infected THP‑1 cells indicated that nucleotide‑binding domain, leucine‑rich repeat containing protein 3 (NLRP3) recruits the adaptor apoptosis‑associated speck‑like protein and caspase‑1 to form an NLRP3 inflammasome complex, crucial for the induction of IL‑1β. In HTNV‑infected THP‑1 cells, reactive oxygen species release, but not extracellular adenosine triphosphate, was crucial for IL‑1β production. In conclusion, Hantavirus induces the formation of the NLRP3 inflammasome in THP‑1 cells and this may be responsible for the elevated IL‑1β levels in HFRS patients.

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