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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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June-2015 Volume 35 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells

  • Authors:
    • Peng Cheng
    • Shuying Zhu
    • Li Jun
    • Lihua Huang
    • Yahui Hong
  • View Affiliations / Copyright

    Affiliations: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China
  • Pages: 1715-1719
    |
    Published online on: April 9, 2015
       https://doi.org/10.3892/ijmm.2015.2179
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Abstract

The aim of the present study was to produce the human dual specificity phosphatase 1 (DUSP1) protein with biological activity and to investigate its in vitro effects on cancer cells. DUSP1 protein was expressed in the baculovirus expression system and purified by Ni-affinity chromatography followed by dialysis in PBS. The purified protein was verified by SDS-PAGE and western blot analysis. Six cancer cell lines were then cultured in the presence of DUSP1 for various periods of time, and the phosphorylated extracellular signal-regulated kinase (p-ERK) content in each cell line was subsequently determined by western blot analysis. Compared to the β-actin level, the amount of p-ERK markedly decreased after 1 h, indicating that DUSP1 suppressed the expression of p-ERK in 6 cancer cell lines examined. Human cervical cancer cells were also collected and counted following co-culture with DUSP1 to examine its effect on the growth rate of cancer cells. A baculovirus expression system for the production of DUSP1 protein was successfully constructed. The p-ERK content was found to be significantly decreased when the cancer cell lines were exposed to DUSP1. The capability of binary fission was reduced when the cells were examined under a microscope. The proliferation of human cervical cancer cells was also inhibited by DUSP1.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng P, Zhu S, Jun L, Huang L and Hong Y: Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells. Int J Mol Med 35: 1715-1719, 2015.
APA
Cheng, P., Zhu, S., Jun, L., Huang, L., & Hong, Y. (2015). Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells. International Journal of Molecular Medicine, 35, 1715-1719. https://doi.org/10.3892/ijmm.2015.2179
MLA
Cheng, P., Zhu, S., Jun, L., Huang, L., Hong, Y."Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells". International Journal of Molecular Medicine 35.6 (2015): 1715-1719.
Chicago
Cheng, P., Zhu, S., Jun, L., Huang, L., Hong, Y."Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells". International Journal of Molecular Medicine 35, no. 6 (2015): 1715-1719. https://doi.org/10.3892/ijmm.2015.2179
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng P, Zhu S, Jun L, Huang L and Hong Y: Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells. Int J Mol Med 35: 1715-1719, 2015.
APA
Cheng, P., Zhu, S., Jun, L., Huang, L., & Hong, Y. (2015). Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells. International Journal of Molecular Medicine, 35, 1715-1719. https://doi.org/10.3892/ijmm.2015.2179
MLA
Cheng, P., Zhu, S., Jun, L., Huang, L., Hong, Y."Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells". International Journal of Molecular Medicine 35.6 (2015): 1715-1719.
Chicago
Cheng, P., Zhu, S., Jun, L., Huang, L., Hong, Y."Production of DUSP1 protein using the baculovirus insect cell expression system and its in vitro effects on cancer cells". International Journal of Molecular Medicine 35, no. 6 (2015): 1715-1719. https://doi.org/10.3892/ijmm.2015.2179
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