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Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis

  • Authors:
    • Xiangcheng Xiao
    • Wenbin Tang
    • Qiongjing Yuan
    • Ling Peng
    • Pingping Yu
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Xiangya Hospital, Central South University, Changsha, Hunan 410000, P.R. China, The Nephrotic Laboratory of Xiangya Hospital, Central South University, Changsha, Hunan 410000, P.R. China, Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, Hunan 410000, P.R. China
    Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1596-1602
    |
    Published online on: April 20, 2015
       https://doi.org/10.3892/ijmm.2015.2189
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Abstract

Krüppel-like factor 4 (KLF4) is a transcription factor which plays divergent roles in a number of physiological or pathological process. However, the expression and role of KLF4 in renal fibrosis remain undetermined. The aim of the present study was to determine the epigenetic alterations of KLF4 and its potential role and mechanisms of action in epithelial-to-mesenchymal transition (EMT) in renal fibrosis. The hypermethylation of the KLF4 promoter accompanied by a decrease in KLF4 expression were observed in mice subjected to unilateral ureteral obstruction (UUO) and in HK-2 cells stimulated with transforming growth factor (TGF)‑β1. However, treatment with 5-aza-2'-deoxycytidine attenuated the TGF-β1-induced downregulation of KLF4 and E-cadherin and the upregulation of α-smooth muscle actin (α-SMA) in the HK-2 cells. DNA methyltransferase 1 (Dnmt1) participated in the TGF-β1-mediated hypermethylation of the KLF4 promoter in the HK-2 cells. In addition, functional analysis demonstrated that the overexpression of KLF4 led to an increase in the expression of E-cadherin and zonula occludens-l (ZO-1), and a decrease in the expression of α-SMA and fibroblast-specific protein 1 (FSP-1), thus reversing the effects of the suppression of KLF4. These data suggest that KLF4 inhibits the progression of EMT in renal epithelial cells. In conclusion, our findings demonstrate that KLF4 is downregulated during EMT in renal fibrosis in vivo and in vitro; thus, KLF4 functions as a suppressor of renal fibrogenesis. The hypermethylation of KLF4 directly mediated by Dnmt1 contributes to the progression of EMT in renal epithelial cells. KLF4 promoter methylation may thus be a promising diagnostic marker or therapeutic target in renal fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao X, Tang W, Yuan Q, Peng L and Yu P: Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis. Int J Mol Med 35: 1596-1602, 2015.
APA
Xiao, X., Tang, W., Yuan, Q., Peng, L., & Yu, P. (2015). Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis. International Journal of Molecular Medicine, 35, 1596-1602. https://doi.org/10.3892/ijmm.2015.2189
MLA
Xiao, X., Tang, W., Yuan, Q., Peng, L., Yu, P."Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis". International Journal of Molecular Medicine 35.6 (2015): 1596-1602.
Chicago
Xiao, X., Tang, W., Yuan, Q., Peng, L., Yu, P."Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis". International Journal of Molecular Medicine 35, no. 6 (2015): 1596-1602. https://doi.org/10.3892/ijmm.2015.2189
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao X, Tang W, Yuan Q, Peng L and Yu P: Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis. Int J Mol Med 35: 1596-1602, 2015.
APA
Xiao, X., Tang, W., Yuan, Q., Peng, L., & Yu, P. (2015). Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis. International Journal of Molecular Medicine, 35, 1596-1602. https://doi.org/10.3892/ijmm.2015.2189
MLA
Xiao, X., Tang, W., Yuan, Q., Peng, L., Yu, P."Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis". International Journal of Molecular Medicine 35.6 (2015): 1596-1602.
Chicago
Xiao, X., Tang, W., Yuan, Q., Peng, L., Yu, P."Epigenetic repression of Krüppel-like factor 4 through Dnmt1 contributes to EMT in renal fibrosis". International Journal of Molecular Medicine 35, no. 6 (2015): 1596-1602. https://doi.org/10.3892/ijmm.2015.2189
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