Arg972 insulin receptor substrate-1 inhibits endothelial nitric oxide synthase expression in human endothelial cells by upregulating microRNA-155

  • Authors:
    • Cheng Huang
    • Guang Li
    • Haojian Dong
    • Shuo Sun
    • Haimin Chen
    • Demou Luo
    • Ling Sun
    • Xida Li
    • Zhujun Chen
    • Huijian Yang
    • Shuisheng Wei
    • Yingling Zhou
  • View Affiliations

  • Published online on: April 21, 2015     https://doi.org/10.3892/ijmm.2015.2192
  • Pages: 239-248
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Abstract

The dysregulation of nitric oxide (NO) synthesis attributable to the abnormal expression/activity of endothelial NO synthase (eNOS) is considered to be a major characteristic of insulin-resistant states, as well as an essential contributor to the pathogenesis of cardiovascular diseases. The Arg972 insulin receptor substrate-1 (IRS-1) is associated with insulin resistance. In the present study, we investigated the association between Arg972 IRS-1 and eNOS expression/activity in human subjects and in primary cultures of human endothelial cells. Data from 832 human subjects revealed that heterozygous and homozygous Arg972 IRS-1 carriers had significantly lower levels of plasma eNOS and nitrite/nitrate than the homozygous wild-type (WT) IRS-1 carriers. Human umbilical vein endothelial cells (HUVECs) established from delivering mothers expressing heterozygous Arg972 IRS-1 had significantly lower eNOS expression/activity and higher miR-155 levels than those expressing WT homozygous IRS-1. The overexpression of IRS-1 and Arg972 IRS-1 in the HUVECs, respectively, decreased and increased the miR-155 expression level. In addition, the overexpression of IRS-1 in the HUVECs significantly increased eNOS expression; this effect was reversed by transfection with mature miR-155 mimic or treatment with the selective phosphatidylinositol-3 kinase (PI3K) inhibitor, BKM120. On the other hand, the overexpression of Arg972 IRS-1 markedly decreased eNOS expression and this effect was reversed by transfection with antagomir-155. On the whole, our in vivo data demonstrate that Arg972 IRS-1 is associated with decreased plasma eNOS and nitrite/nitrate levels in human subjects. Our in vitro data demonstrate that Arg972 IRS-1 inhibits eNOS expression in human endothelial cells by upregulating miR-155 expression through the impairment of PI3K signaling. The present study provides new insight into the pathophysiological role of Arg972 IRS-1 in cardiovascular diseases.
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July-2015
Volume 36 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Huang C, Li G, Dong H, Sun S, Chen H, Luo D, Sun L, Li X, Chen Z, Yang H, Yang H, et al: Arg972 insulin receptor substrate-1 inhibits endothelial nitric oxide synthase expression in human endothelial cells by upregulating microRNA-155. Int J Mol Med 36: 239-248, 2015
APA
Huang, C., Li, G., Dong, H., Sun, S., Chen, H., Luo, D. ... Zhou, Y. (2015). Arg972 insulin receptor substrate-1 inhibits endothelial nitric oxide synthase expression in human endothelial cells by upregulating microRNA-155. International Journal of Molecular Medicine, 36, 239-248. https://doi.org/10.3892/ijmm.2015.2192
MLA
Huang, C., Li, G., Dong, H., Sun, S., Chen, H., Luo, D., Sun, L., Li, X., Chen, Z., Yang, H., Wei, S., Zhou, Y."Arg972 insulin receptor substrate-1 inhibits endothelial nitric oxide synthase expression in human endothelial cells by upregulating microRNA-155". International Journal of Molecular Medicine 36.1 (2015): 239-248.
Chicago
Huang, C., Li, G., Dong, H., Sun, S., Chen, H., Luo, D., Sun, L., Li, X., Chen, Z., Yang, H., Wei, S., Zhou, Y."Arg972 insulin receptor substrate-1 inhibits endothelial nitric oxide synthase expression in human endothelial cells by upregulating microRNA-155". International Journal of Molecular Medicine 36, no. 1 (2015): 239-248. https://doi.org/10.3892/ijmm.2015.2192