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Isolation of extracellular vesicles: Determining the correct approach (Review)

  • Authors:
    • Rafal Szatanek
    • Jarek Baran
    • Maciej Siedlar
    • Monika Baj-Krzyworzeka
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Immunology and Transplantology, Jagiellonian University Medical College, 30-663 Krakow, Poland
    Copyright: © Szatanek et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 11-17
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    Published online on: April 22, 2015
       https://doi.org/10.3892/ijmm.2015.2194
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Abstract

The discovery of extracellular vesicles (EVs) has revised the interpretation of intercellular communication. It is now well established that EVs play a significant role in coagulation, inflammation, cancer and stem cell renewal and expansion. Their release presents an intriguing, transporting/trafficking network of biologically active molecules, which are able to reach and modulate the function/behavior of the target cells in a variety of ways. Moreover, the presence of EVs in various body fluids points to their potential for use as biomarkers and prognostic indicators in the surveillance/monitoring of a variety of diseases. Although vast knowledge on the subject of EVs has accumulated over the years, there are still fundamental issues associated with the correct approach for their isolation. This review comprises the knowledge on EV isolation techniques that are currently available. The aim of this reveiw was to make both experienced researchers and newcomers to the field aware that different types of EVs require unique isolation approaches. The realization of this ‘uniqueness’ is the first step in the right direction for the complete assessment of EVs.
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1 

D’Souza-Schorey C and Clancy JW: Tumor-derived microvesicles: Shedding light on novel microenvironment modulators and prospective cancer biomarkers. Genes Dev. 26:1287–1299. 2012. View Article : Google Scholar

2 

Ratajczak J, Miekus K, Kucia M, Zhang J, Reca R, Dvorak P and Ratajczak MZ: Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: Evidence for horizontal transfer of mRNA and protein delivery. Leukemia. 20:847–856. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Mause SF and Weber C: Microparticles: Protagonists of a novel communication network for intercellular information exchange. Circ Res. 107:1047–1057. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Ratajczak J, Wysoczynski M, Hayek F, Janowska-Wieczorek A and Ratajczak MZ: Membrane-derived microvesicles: Important and underappreciated mediators of cell-to-cell communication. Leukemia. 20:1487–1495. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Cocucci E, Racchetti G and Meldolesi J: Shedding microvesicles: Artefacts no more. Trends Cell Biol. 19:43–51. 2009. View Article : Google Scholar : PubMed/NCBI

6 

van der Pol E, Hoekstra AG, Sturk A, Otto C, van Leeuwen TG and Nieuwland R: Optical and non-optical methods for detection and characterization of microparticles and exosomes. J Thromb Haemost. 8:2596–2607. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Valadi H, Ekström K, Bossios A, Sjöstrand M, Lee JJ and Lötvall JO: Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 9:654–659. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Baj-Krzyworzeka M, Szatanek R, Weglarczyk K, Baran J, Urbanowicz B, Brański P, Ratajczak MZ and Zembala M: Tumour-derived microvesicles carry several surface determinants and mRNA of tumour cells and transfer some of these determinants to monocytes. Cancer Immunol Immunother. 55:808–818. 2006. View Article : Google Scholar

9 

Al-Nedawi K, Meehan B, Micallef J, Lhotak V, May L, Guha A and Rak J: Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells. Nat Cell Biol. 10:619–624. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Vlassov AV, Magdaleno S, Setterquist R and Conrad R: Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. Biochim Biophys Acta. 1820:940–948. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Simpson RJ, Jensen SS and Lim JW: Proteomic profiling of exosomes: Current perspectives. Proteomics. 8:4083–4099. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM and Falcon-Perez JM: Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes. J Proteome Res. 7:5157–5166. 2008. View Article : Google Scholar

13 

Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, et al: Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins. J Lipid Res. 51:2105–2120. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Mathivanan S and Simpson RJ: ExoCarta: A compendium of exosomal proteins and RNA. Proteomics. 9:4997–5000. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Aharon A, Rebibo-Sabbah A, Tzoran I and Levin C: Extracellular vesicles in hematological disorders. Rambam Maimonides Med J. 5:e00322014. View Article : Google Scholar : PubMed/NCBI

16 

Inal JM, Kosgodage U, Azam S, Stratton D, Antwi-Baffour S and Lange S: Blood/plasma secretome and microvesicles. Biochim Biophys Acta. 1834:2317–2325. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Piccin A, Murphy WG and Smith OP: Circulating microparticles: Pathophysiology and clinical implications. Blood Rev. 21:157–171. 2007. View Article : Google Scholar

18 

Lynch SF and Ludlam CA: Plasma microparticles and vascular disorders. Br J Haematol. 137:36–48. 2007.PubMed/NCBI

19 

Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjøt L, Orntoft TF, Howard KA and Ostenfeld MS: Comparative analysis of discrete exosome fractions obtained by differential centrifugation. J Extracell Vesicles. 3:250112014. View Article : Google Scholar : PubMed/NCBI

20 

Théry C, Amigorena S, Raposo G and Clayton A: Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol. Chapter 3: Unit 3. 22:2006. View Article : Google Scholar

21 

Lötvall J, Hill AF, Hochberg F, Buzás EI, Di Vizio D, Gardiner C, Gho YS, Kurochkin IV, Mathivanan S, Quesenberry P, et al: Minimal experimental requirements for definition of extracellular vesicles and their functions: A position statement from the International Society for Extracellular Vesicles. J Extracell Vesicles. 3:269132014. View Article : Google Scholar : PubMed/NCBI

22 

Vickers KC, Palmisano BT, Shoucri BM, Shamburek RD and Remaley AT: MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nat Cell Biol. 13:423–433. 2011. View Article : Google Scholar : PubMed/NCBI

23 

György B, Módos K, Pállinger E, Pálóczi K, Pásztói M, Misják P, Deli MA, Sipos A, Szalai A, Voszka I, et al: Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood. 117:e39–e48. 2011. View Article : Google Scholar

24 

Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss DS, Weinrauch Y and Zychlinsky A: Neutrophil extracellular traps kill bacteria. Science. 303:1532–1535. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Williams JC and Mackman N: MPs or ICs? Blood. 117:1101–1102. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Phillipson M and Kubes P: The neutrophil in vascular inflammation. Nat Med. 17:1381–1390. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Gheldof D, Hardij J, Cecchet F, Chatelain B, Dogné JM and Mullier F: Thrombin generation assay and transmission electron microscopy: A useful combination to study tissue factor-bearing microvesicles. J Extracell Vesicles. 2:22013. View Article : Google Scholar

28 

Lacroix R, Judicone C, Mooberry M, Boucekine M and Key NS: Standardization of pre-analytical variables in plasma microparticle determination: Results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop. J Thromb Haemost. Apr 2–2013.Epub ahead of print. View Article : Google Scholar

29 

Yuana Y, Bertina RM and Osanto S: Pre-analytical and analytical issues in the analysis of blood microparticles. Thromb Haemost. 105:396–408. 2011. View Article : Google Scholar

30 

Witwer KW, Buzás EI, Bemis LT, Bora A, Lässer C, Lötvall J, Nolte-’t Hoen EN, Piper MG, Sivaraman S, Skog J, et al: Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. Extracell Vesicles. 2:203602013.

31 

Ayers L, Kohler M, Harrison P, Sargent I, Dragovic R, Schaap M, Nieuwland R, Brooks SA and Ferry B: Measurement of circulating cell-derived microparticles by flow cytometry: Sources of variability within the assay. Thromb Res. 127:370–377. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Jayachandran M, Miller VM, Heit JA and Owen WG: Methodology for isolation, identification and characterization of microvesicles in peripheral blood. J Immunol Methods. 375:207–214. 2012. View Article : Google Scholar :

33 

Chandler WL, Yeung W and Tait JF: A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer. J Thromb Haemost. 9:1216–1224. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Montoro-García S, Shantsila E, Tapp LD, López-Cuenca A, Romero AI, Hernández-Romero D, Orenes-Piñero E, Manzano-Fernández S, Valdés M, Marín F and Lip GY: Small-size circulating microparticles in acute coronary syndromes: Relevance to fibrinolytic status, reparative markers and outcomes. Atherosclerosis. 227:313–322. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Sokolova V, Ludwig AK, Hornung S, Rotan O, Horn PA, Epple M and Giebel B: Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy. Colloids Surf B Biointerfaces. 87:146–150. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Trummer A, De Rop C, Tiede A, Ganser A and Eisert R: Recovery and composition of microparticles after snap-freezing depends on thawing temperature. Blood Coagul Fibrinolysis. 20:52–56. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Zhou H, Yuen PS, Pisitkun T, Gonzales PA, Yasuda H, Dear JW, Gross P, Knepper MA and Star RA: Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery. Kidney Int. 69:1471–1476. 2006.PubMed/NCBI

38 

Raposo G, Nijman HW, Stoorvogel W, Liejendekker R, Harding CV, Melief CJ and Geuze HJ: B lymphocytes secrete antigen-presenting vesicles. J Exp Med. 183:1161–1172. 1996. View Article : Google Scholar : PubMed/NCBI

39 

Théry C, Ostrowski M and Segura E: Membrane vesicles as conveyors of immune responses. Nat Rev Immunol. 9:581–593. 2009. View Article : Google Scholar : PubMed/NCBI

40 

Baran J, Baj-Krzyworzeka M, Weglarczyk K, Szatanek R and Zembala M, Barbasz J, Czupryna A, Szczepanik A and Zembala M: Circulating tumour-derived microvesicles in plasma of gastric cancer patients. Cancer Immunol Immunother. 59:841–850. 2010. View Article : Google Scholar : PubMed/NCBI

41 

Abas L and Luschnig C: Maximum yields of microsomal-type membranes from small amounts of plant material without requiring ultracentrifugation. Anal Biochem. 401:217–227. 2010. View Article : Google Scholar : PubMed/NCBI

42 

Lavoie C, Lanoix J, Kan FW and Paiement J: Cell-free assembly of rough and smooth endoplasmic reticulum. J Cell Sci. 109:1415–1425. 1996.PubMed/NCBI

43 

Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC and Lambrecht BN: Proteomic analysis of exosomes isolated from human malignant pleural effusions. Am J Respir Cell Mol Biol. 31:114–121. 2004. View Article : Google Scholar : PubMed/NCBI

44 

Keller S, Ridinger J, Rupp AK, Janssen JW and Altevogt P: Body fluid derived exosomes as a novel template for clinical diagnostics. J Transl Med. 9:862011. View Article : Google Scholar : PubMed/NCBI

45 

Poliakov A, Spilman M, Dokland T, Amling CL and Mobley JA: Structural heterogeneity and protein composition of exosome-like vesicles (prostasomes) in human semen. Prostate. 69:159–167. 2009. View Article : Google Scholar

46 

Dettenhofer M and Yu XF: Highly purified human immunodeficiency virus type 1 reveals a virtual absence of Vif in virions. J Virol. 73:1460–1467. 1999.PubMed/NCBI

47 

Cantin R, Diou J, Bélanger D, Tremblay AM and Gilbert C: Discrimination between exosomes and HIV-1: Purification of both vesicles from cell-free supernatants. J Immunol Methods. 338:21–30. 2008. View Article : Google Scholar : PubMed/NCBI

48 

Yuana Y, Levels J, Grootemaat A, Sturk A and Nieuwland R: Co-isolation of extracellular vesicles and high-density lipoproteins using density gradient ultracentrifugation. J Extracell Vesicles. 3:32014. View Article : Google Scholar

49 

Fitzner D, Schnaars M, van Rossum D, Krishnamoorthy G, Dibaj P, Bakhti M, Regen T, Hanisch UK and Simons M: Selective transfer of exosomes from oligodendrocytes to microglia by macropinocytosis. J Cell Sci. 124:447–458. 2011. View Article : Google Scholar : PubMed/NCBI

50 

Müller G: Novel tools study cell type-specific exosomes microvesicles. J Bioanal Biomed. 4:46–60. 2012.

51 

Taylor DD, Lyons KS and Gerçel-Taylor C: Shed membrane fragment-associated markers for endometrial and ovarian cancers. Gynecol Oncol. 84:443–448. 2002. View Article : Google Scholar : PubMed/NCBI

52 

Böing AN, van der Pol E, Grootemaat AE, Coumans FA, Sturk A and Nieuwland R: Single-step isolation of extracellular vesicles by size-exclusion chromatography. J Extracell Vesicles. 3:32014. View Article : Google Scholar

53 

Alvarez ML, Khosroheidari M, Kanchi Ravi R and DiStefano JK: Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers. Kidney Int. 82:1024–1032. 2012. View Article : Google Scholar : PubMed/NCBI

54 

Taylor DD, Zacharias W and Gerçel-Taylor C: Exosome isolation for proteomic analyses and RNA profiling. Methods Mol Biol. 728:235–246. 2011. View Article : Google Scholar : PubMed/NCBI

55 

Danesh A, Inglis HC, Jackman RP, Wu S, Deng X, Muench MO, Heitman JW and Norris PJ: Exosomes from red blood cell units bind to monocytes and induce proinflammatory cytokines, boosting T-cell responses in vitro. Blood. 123:687–696. 2014. View Article : Google Scholar :

56 

de Vrij J, Maas SL, Kwappenberg KM, Schnoor R, Kleijn A, Dekker L, Luider TM, de Witte LD, Litjens M, van Strien ME, et al: Glioblastoma-derived extracellular vesicles modify the phenotype of monocytic cells. Int J Cancer. Mar 20–2015.Epub ahead of print. View Article : Google Scholar : PubMed/NCBI

57 

Ismail N, Wang Y, Dakhlallah D, Moldovan L, Agarwal K, Batte K, Shah P, Wisler J, Eubank TD, Tridandapani S, et al: Macrophage microvesicles induce macrophage differentiation and miR-223 transfer. Blood. 121:984–995. 2013. View Article : Google Scholar :

58 

Sáenz-Cuesta M, Arbelaiz A, Oregi A, Irizar H, Osorio-Querejeta I, Muñoz-Culla M, Banales JM, Falcón-Pérez JM, Olascoaga J and Otaegui D: Methods for extracellular vesicles isolation in a hospital setting. Front Immunol. 6:502015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Szatanek R, Baran J, Siedlar M and Baj-Krzyworzeka M: Isolation of extracellular vesicles: Determining the correct approach (Review). Int J Mol Med 36: 11-17, 2015.
APA
Szatanek, R., Baran, J., Siedlar, M., & Baj-Krzyworzeka, M. (2015). Isolation of extracellular vesicles: Determining the correct approach (Review). International Journal of Molecular Medicine, 36, 11-17. https://doi.org/10.3892/ijmm.2015.2194
MLA
Szatanek, R., Baran, J., Siedlar, M., Baj-Krzyworzeka, M."Isolation of extracellular vesicles: Determining the correct approach (Review)". International Journal of Molecular Medicine 36.1 (2015): 11-17.
Chicago
Szatanek, R., Baran, J., Siedlar, M., Baj-Krzyworzeka, M."Isolation of extracellular vesicles: Determining the correct approach (Review)". International Journal of Molecular Medicine 36, no. 1 (2015): 11-17. https://doi.org/10.3892/ijmm.2015.2194
Copy and paste a formatted citation
x
Spandidos Publications style
Szatanek R, Baran J, Siedlar M and Baj-Krzyworzeka M: Isolation of extracellular vesicles: Determining the correct approach (Review). Int J Mol Med 36: 11-17, 2015.
APA
Szatanek, R., Baran, J., Siedlar, M., & Baj-Krzyworzeka, M. (2015). Isolation of extracellular vesicles: Determining the correct approach (Review). International Journal of Molecular Medicine, 36, 11-17. https://doi.org/10.3892/ijmm.2015.2194
MLA
Szatanek, R., Baran, J., Siedlar, M., Baj-Krzyworzeka, M."Isolation of extracellular vesicles: Determining the correct approach (Review)". International Journal of Molecular Medicine 36.1 (2015): 11-17.
Chicago
Szatanek, R., Baran, J., Siedlar, M., Baj-Krzyworzeka, M."Isolation of extracellular vesicles: Determining the correct approach (Review)". International Journal of Molecular Medicine 36, no. 1 (2015): 11-17. https://doi.org/10.3892/ijmm.2015.2194
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