Effects of paracrine factors on CD24 expression and neural differentiation of male germline stem cells

  • Authors:
    • Bang-Jin Kim
    • Yong-An Lee
    • Ki-Jung Kim
    • Yong-Hee Kim
    • Mi-Seon Jung
    • Seung-Jung Ha
    • Hyun-Gu Kang
    • Sang-Eun Jung
    • Byung-Gak Kim
    • Yu-Ri Choi
    • Jeong Tae Do
    • Buom-Yong Ryu
  • View Affiliations

  • Published online on: May 12, 2015     https://doi.org/10.3892/ijmm.2015.2208
  • Pages: 255-262
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Abstract

Spermatogonial stem cells (SSCs) are adult male germ cells that develop after birth. Throughout the lifetime of an organism, SSCs sustain spermatogenesis through self-renewal and produce daughter cells that differentiate into spermatozoa. Several studies have demonstrated that SSCs can acquire pluripotency under appropriate culture conditions, thus becoming multipotent germline stem cells (mGSCs) that express markers of pluripotency in culture and form teratomas following transplantation into immunodeficient mice. In the present study, we generated neural precursor cells expressing CD24, a neural precursor marker, from pluripotent stem cell lines and demonstrated that these cells effectively differentiated along a neural lineage in vitro. In addition, we found that paracrine factors promoted CD24 expression during the neural differentiation of mGSCs. Our results indicated that the expression of CD24, enhanced by a combination of retinoic acid (RA), noggin and fibroblast growth factor 8 (FGF8) under serum-free conditions promoted neural precursor differentiation. Using a simple cell sorting method, we were able to collect neural precursor cells with the potential to differentiate from mGSCs into mature neurons and astrocytes in vitro.
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July-2015
Volume 36 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim B, Lee Y, Kim K, Kim Y, Jung M, Ha S, Kang H, Jung S, Kim B, Choi Y, Choi Y, et al: Effects of paracrine factors on CD24 expression and neural differentiation of male germline stem cells. Int J Mol Med 36: 255-262, 2015
APA
Kim, B., Lee, Y., Kim, K., Kim, Y., Jung, M., Ha, S. ... Ryu, B. (2015). Effects of paracrine factors on CD24 expression and neural differentiation of male germline stem cells. International Journal of Molecular Medicine, 36, 255-262. https://doi.org/10.3892/ijmm.2015.2208
MLA
Kim, B., Lee, Y., Kim, K., Kim, Y., Jung, M., Ha, S., Kang, H., Jung, S., Kim, B., Choi, Y., Do, J. T., Ryu, B."Effects of paracrine factors on CD24 expression and neural differentiation of male germline stem cells". International Journal of Molecular Medicine 36.1 (2015): 255-262.
Chicago
Kim, B., Lee, Y., Kim, K., Kim, Y., Jung, M., Ha, S., Kang, H., Jung, S., Kim, B., Choi, Y., Do, J. T., Ryu, B."Effects of paracrine factors on CD24 expression and neural differentiation of male germline stem cells". International Journal of Molecular Medicine 36, no. 1 (2015): 255-262. https://doi.org/10.3892/ijmm.2015.2208