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International Journal of Molecular Medicine
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Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines

  • Authors:
    • Alena Nunukova
    • Jakub Neradil
    • Jan Skoda
    • Josef Jaros
    • Ales Hampl
    • Jaroslav Sterba
    • Renata Veselska
  • View Affiliations / Copyright

    Affiliations: Department of Experimental Biology, Faculty of Science, Masaryk University, 61137 Brno, Czech Republic, Department of Histology and Embryology, Faculty of Medicine, Masaryk University, 61137 Brno, Czech Republic, Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, 61137 Brno, Czech Republic
    Copyright: © Nunukova et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 65-72
    |
    Published online on: May 14, 2015
       https://doi.org/10.3892/ijmm.2015.2210
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Abstract

CD133 (also known as prominin-1) is a cell surface glycoprotein that is widely used for the identification of stem cells. Furthermore, its glycosylated epitope, AC133, has recently been discussed as a marker of cancer stem cells in various human malignancies. During our recent experiments on rhabdomyosarcomas (RMS), we unexpectedly identified an atypical nuclear localization of CD133 in a relatively stable subset of cells in five RMS cell lines established in our laboratory. To the best of our knowledge, this atypical localization of CD133 has not yet been proven or analyzed in detail in cancer cells. In the present study, we verified the nuclear localization of CD133 in RMS cells using three independent anti-CD133 antibodies, including both rabbit polyclonal and mouse monoclonal antibodies. Indirect immunofluorescence and confocal microscopy followed by software cross-section analysis, transmission electron microscopy and cell fractionation with immunoblotting were also employed, and all the results undeniably confirmed the presence of CD133 in the nuclei of stable minor subpopulations of all five RMS cell lines. The proportion of cells showing an exclusive nuclear localization of CD133 ranged from 3.4 to 7.5%, with only minor differences observed among the individual anti-CD133 antibodies. Although the role of CD133 in the cell nucleus remains unclear, these results clearly indicate that this atypical nuclear localization of CD133 in a minor subpopulation of cancer cells is a common phenomenon in RMS cell lines.
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1 

Corbeil D, Karbanová J, Fargeas CA and Jászai J: Prominin-1 (CD133): Molecular and cellular features across species. Adv Exp Med Biol. 777:3–24. 2013. View Article : Google Scholar

2 

Grosse-Gehling P, Fargeas CA, Dittfeld C, Garbe Y, Alison MR, Corbeil D and Kunz-Schughart LA: CD133 as a biomarker for putative cancer stem cells in solid tumours: Limitations, problems and challenges. J Pathol. 229:355–378. 2013. View Article : Google Scholar

3 

Friedman GK and Gillespie GY: Cancer stem cells and pediatric solid tumors. Cancers (Basel). 3:298–318. 2011. View Article : Google Scholar

4 

Xia P: Surface markers of cancer stem cells in solid tumors. Curr Stem Cell Res Ther. 9:102–111. 2014. View Article : Google Scholar

5 

Veselska R, Hermanova M, Loja T, Chlapek P, Zambo I, Vesely K, Zitterbart K and Sterba J: Nestin expression in osteosarcomas and derivation of nestin/CD133 positive osteosarcoma cell lines. BMC Cancer. 8:3002008. View Article : Google Scholar : PubMed/NCBI

6 

Sana J, Zambo I, Skoda J, Neradil J, Chlapek P, Hermanova M, Mudry P, Vasikova A, Zitterbart K, Hampl A, et al: CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines. Anal Cell Pathol (Amst). 34:303–318. 2011. View Article : Google Scholar

7 

Di Fiore R, Santulli A, Ferrante RD, Giuliano M, De Blasio A, Messina C, Pirozzi G, Tirino V, Tesoriere G and Vento R: Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment. J Cell Physiol. 219:301–313. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Tirino V, Desiderio V, Paino F, De Rosa A, Papaccio F, Fazioli F, Pirozzi G and Papaccio G: Human primary bone sarcomas contain CD133+ cancer stem cells displaying high tumorigenicity in vivo. FASEB J. 25:2022–2030. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Walter D, Satheesha S, Albrecht P, Bornhauser BC, D’Alessandro V, Oesch SM, Rehrauer H, Leuschner I, Koscielniak E, Gengler C, et al: CWS Study Group: CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres. PLoS One. 6:e195062011. View Article : Google Scholar

10 

Pressey JG, Haas MC, Pressey CS, Kelly VM, Parker JN, Gillespie GY and Friedman GK: CD133 marks a myogenically primitive subpopulation in rhabdomyosarcoma cell lines that are relatively chemoresistant but sensitive to mutant HSV. Pediatr Blood Cancer. 60:45–52. 2013. View Article : Google Scholar

11 

Veselska R, Skoda J and Neradil J: Detection of cancer stem cell markers in sarcomas. Klin Onkol. 25:2S16–2S20. 2012.PubMed/NCBI

12 

Dela Cruz FS: Cancer stem cells in pediatric sarcomas. Front Oncol. 3:1682013. View Article : Google Scholar : PubMed/NCBI

13 

Satheesha S and Schafer BW: Cancer stem cells in pediatric sarcomas. Stem Cells and Cancer Stem Cells. Hayat MA: Springer; Dordrecht: pp. 111–126. 2014, View Article : Google Scholar

14 

Cantile M, Collina F, D’Aiuto M, Rinaldo M, Pirozzi G, Borsellino C, Franco R, Botti G and Di Bonito M: Nuclear localization of cancer stem cell marker CD133 in triple-negative breast cancer: A case report. Tumori. 99:e245–e250. 2013.PubMed/NCBI

15 

Huang M, Zhu H, Feng J, Ni S and Huang J: High CD133 expression in the nucleus and cytoplasm predicts poor prognosis in non-small cell lung cancer. Dis Markers. 2015:9860952015. View Article : Google Scholar : PubMed/NCBI

16 

Veselska R, Kuglik P, Cejpek P, Svachova H, Neradil J, Loja T and Relichova J: Nestin expression in the cell lines derived from glioblastoma multiforme. BMC Cancer. 6:322006. View Article : Google Scholar : PubMed/NCBI

17 

Bidlingmaier S, Zhu X and Liu B: The utility and limitations of glycosylated human CD133 epitopes in defining cancer stem cells. J Mol Med Berl. 86:1025–1032. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Loja T, Chlapek P, Kuglik P, Pesakova M, Oltova A, Cejpek P and Veselska R: Characterization of a GM7 glioblastoma cell line showing CD133 positivity and both cytoplasmic and nuclear localization of nestin. Oncol Rep. 21:119–127. 2009.

19 

Tirino V, Desiderio V, d’Aquino R, De Francesco F, Pirozzi G, Graziano A, Galderisi U, Cavaliere C, De Rosa A, Papaccio G and Giordano A: Detection and characterization of CD133+ cancer stem cells in human solid tumours. PLoS One. 3:e34692008. View Article : Google Scholar

20 

Mak AB, Nixon AM, Kittanakom S, Stewart JM, Chen GI, Curak J, Gingras AC, Mazitschek R, Neel BG, Stagljar I and Moffat J: Regulation of CD133 by HDAC6 promotes β-catenin signaling to suppress cancer cell differentiation. Cell Rep. 2:951–963. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Wang SC and Hung MC: Nuclear translocation of the epidermal growth factor receptor family membrane tyrosine kinase receptors. Clin Cancer Res. 15:6484–6489. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Mills IG: Nuclear translocation and functions of growth factor receptors. Semin Cell Dev Biol. 23:165–171. 2012. View Article : Google Scholar

23 

Carpenter G and Liao HJ: Receptor tyrosine kinases in the nucleus. Cold Spring Harb Perspect Biol. 5:a0089792013. View Article : Google Scholar : PubMed/NCBI

24 

Takenobu H, Shimozato O, Nakamura T, Ochiai H, Yamaguchi Y, Ohira M, Nakagawara A and Kamijo T: CD133 suppresses neuroblastoma cell differentiation via signal pathway modification. Oncogene. 30:97–105. 2011. View Article : Google Scholar

25 

Wei Y, Jiang Y, Zou F, Liu Y, Wang S, Xu N, Xu W, Cui C, Xing Y, Liu Y, et al: Activation of PI3K/Akt pathway by CD133-p85 interaction promotes tumorigenic capacity of glioma stem cells. Proc Natl Acad Sci USA. 110:6829–6834. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Shimozato O, Waraya M, Nakashima K, Souda H, Takiguchi N, Yamamoto H, Takenobu H, Uehara H, Ikeda E, Matsushita S, et al: Receptor-type protein tyrosine phosphatase κ directly dephosphorylates CD133 and regulates downstream AKT activation. Oncogene. 34:1949–1960. 2014. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Nunukova A, Neradil J, Skoda J, Jaros J, Hampl A, Sterba J and Veselska R: Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines. Int J Mol Med 36: 65-72, 2015.
APA
Nunukova, A., Neradil, J., Skoda, J., Jaros, J., Hampl, A., Sterba, J., & Veselska, R. (2015). Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines. International Journal of Molecular Medicine, 36, 65-72. https://doi.org/10.3892/ijmm.2015.2210
MLA
Nunukova, A., Neradil, J., Skoda, J., Jaros, J., Hampl, A., Sterba, J., Veselska, R."Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines". International Journal of Molecular Medicine 36.1 (2015): 65-72.
Chicago
Nunukova, A., Neradil, J., Skoda, J., Jaros, J., Hampl, A., Sterba, J., Veselska, R."Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines". International Journal of Molecular Medicine 36, no. 1 (2015): 65-72. https://doi.org/10.3892/ijmm.2015.2210
Copy and paste a formatted citation
x
Spandidos Publications style
Nunukova A, Neradil J, Skoda J, Jaros J, Hampl A, Sterba J and Veselska R: Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines. Int J Mol Med 36: 65-72, 2015.
APA
Nunukova, A., Neradil, J., Skoda, J., Jaros, J., Hampl, A., Sterba, J., & Veselska, R. (2015). Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines. International Journal of Molecular Medicine, 36, 65-72. https://doi.org/10.3892/ijmm.2015.2210
MLA
Nunukova, A., Neradil, J., Skoda, J., Jaros, J., Hampl, A., Sterba, J., Veselska, R."Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines". International Journal of Molecular Medicine 36.1 (2015): 65-72.
Chicago
Nunukova, A., Neradil, J., Skoda, J., Jaros, J., Hampl, A., Sterba, J., Veselska, R."Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines". International Journal of Molecular Medicine 36, no. 1 (2015): 65-72. https://doi.org/10.3892/ijmm.2015.2210
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