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Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy

  • Authors:
    • Carmen Huesa
    • Katherine A. Staines
    • Jose Luis Millán
    • Vicky E. MacRae
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    Affiliations: Roslin Institute and R(D)SVS, The University of Edinburgh, Edinburgh, UK, Sanford Children's Health Research Center, Sanford-Burnham Medical Research Institute, La Jolla, CA, USA
    Copyright: © Huesa et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 159-165
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    Published online on: May 15, 2015
       https://doi.org/10.3892/ijmm.2015.2212
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Abstract

Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder of spontaneous infantile arterial and periarticular calcification which is attributed to mutations in the ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1) gene. Whilst the bisphosphonate, etidronate, is currently used off-label for the treatment for GACI, recent studies have highlighted its detrimental effects on bone mineralisation. In the present study, we used the Enpp1-/- mouse model of GACI to examine the effects of etidronate treatment (100 µg/kg), on vascular and skeletal calcification. Micro-computed tomography (µCT) analysis revealed a significant decrease in trabecular bone mass, as reflected by the decrease in trabecular bone volume/tissue volume (BV/TV; %), trabecular thickness, trabecular separation, trabecular number and pattern factor (P<0.05) in the Enpp1-/- mice in comparison to the wild-type (WT) mice. Mechanical testing revealed that in the WT mice, treatment with etidronate significantly improved work to fracture and increased work post-failure (P<0.05, in comparison to the vehicle-treated WT mice). This significant increase, however, was not observed in the Enpp1-/- mice. Treatment with etidronate had no effect on bone parameters in the WT mice; however, the Enpp1-/- mice displayed an increased structural model index (SMI; P<0.05). We used a recently developed 3D µCT protocol to reconstruct and quantify the extensive aortic calcification in Enpp1-/- mice in comparison to the WT mice. However, treatment with etidronate did not prevent de novo calcification, and did not arrest the progression of established calcification of the aorta.
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View References

1 

Rutsch F, Ruf N, Vaingankar S, Toliat MR, Suk A, Höhne W, Schauer G, Lehmann M, Roscioli T, Schnabel D, et al: Mutations in ENPP1 are associated with ‘idiopathic’ infantile arterial calcification. Nat Genet. 34:379–381. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Rutsch F, Vaingankar S, Johnson K, Goldfine I, Maddux B, Schauerte P, Kalhoff H, Sano K, Boisvert WA, Superti-Furga A, et al: PC-1 nucleoside triphosphate pyrophosphohydrolase defi-ciency in idiopathic infantile arterial calcification. Am J Pathol. 158:543–554. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Nitschke Y and Rutsch F: Modulators of networks: Molecular targets of arterial calcification identified in man and mice. Curr Pharm Des. 20:5839–5852. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Mackenzie NC, Huesa C, Rutsch F and MacRae VE: New insights into NPP1 function: Lessons from clinical and animal studies. Bone. 51:961–968. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Mackenzie NC, Zhu D, Milne EM, van’t Hof R, Martin A, Darryl Quarles L, Millán JL, Farquharson C and MacRae VE: Altered bone development and an increase in FGF-23 expression in Enpp1−/−mice. PLoS One. 7:e321772012. View Article : Google Scholar

6 

Apschner A, Huitema LF, Ponsioen B, Peterson-Maduro J and Schulte-Merker S: Zebrafish enpp1 mutants exhibit pathological mineralization, mimicking features of generalized arterial calcification of infancy (GACI) and pseudoxanthoma elasticum (PXE). Dis Model Mech. 7:811–822. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Hajjawi MO, MacRae VE, Huesa C, Boyde A, Millán JL, Arnett TR and Orriss IR: Mineralisation of collagen rich soft tissues and osteocyte lacunae in Enpp1−/−mice. Bone. 69:139–147. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Li Q, Guo H, Chou DW, Berndt A, Sundberg JP and Uitto J: Mutant Enpp1asj mice as a model for generalized arterial calcification of infancy. Dis Model Mech. 6:1227–1235. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Hakim FT, Cranley R, Brown KS, Eanes ED, Harne L and Oppenheim JJ: Hereditary joint disorder in progressive ankylosis (ank/ank) mice. I. Association of calcium hydroxyapatite deposition with inflammatory arthropathy. Arthritis Rheum. 27:1411–1420. 1984. View Article : Google Scholar : PubMed/NCBI

10 

Terkeltaub R, Rosenbach M, Fong F and Goding J: Causal link between nucleotide pyrophosphohydrolase overactivity and increased intracellular inorganic pyrophosphate generation demonstrated by transfection of cultured fibroblasts and osteoblasts with plasma cell membrane glycoprotein-1. Relevance to calcium pyrophosphate dihydrate deposition disease. Arthritis Rheum. 37:934–941. 1994. View Article : Google Scholar : PubMed/NCBI

11 

Addison WN, Azari F, Sørensen ES, Kaartinen MT and McKee MD: Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity. J Biol Chem. 282:15872–15883. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Staines KA, MacRae VE and Farquharson C: The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling. J Endocrinol. 214:241–255. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Anderson HC: Molecular biology of matrix vesicles. Clin Orthop Relat Res. (314): 266–280. 1995.PubMed/NCBI

14 

Moss DW, Eaton RH, Smith JK and Whitby LG: Association of inorganic-pyrophosphatase activity with human alkaline-phosphatase preparations. Biochem J. 102:53–57. 1967.PubMed/NCBI

15 

Majeska RJ and Wuthier RE: Studies on matrix vesicles isolated from chick epiphyseal cartilage. Association of pyrophosphatase and ATPase activities with alkaline phosphatase. Biochim Biophys Acta. 391:51–60. 1975. View Article : Google Scholar : PubMed/NCBI

16 

Hessle L, Johnson KA, Anderson HC, Narisawa S, Sali A, Goding JW, Terkeltaub R and Millan JL: Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization. Proc Natl Acad Sci USA. 99:9445–9449. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Murshed M, Schinke T, McKee MD and Karsenty G: Extracellular matrix mineralization is regulated locally; different roles of two glacontaining proteins. J Cell Biol. 165:625–630. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Macrae VE, Davey MG, McTeir L, Narisawa S, Yadav MC, Millan JL and Farquharson C: Inhibition of PHOSPHO1 activity results in impaired skeletal mineralization during limb development of the chick. Bone. 46:1146–1155. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Roberts S, Narisawa S, Harmey D, Millán JL and Farquharson C: Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization. J Bone Miner Res. 22:617–627. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Roberts SJ, Owen HC and Farquharson C: Identification of a novel splice variant of the haloacid dehalogenase: PHOSPHO1. Biochem Biophys Res Commun. 371:872–876. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Stewart AJ, Roberts SJ, Seawright E, Davey MG, Fleming RH and Farquharson C: The presence of PHOSPHO1 in matrix vesicles and its developmental expression prior to skeletal mineralization. Bone. 39:1000–1007. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Yadav MC, Simão AM, Narisawa S, Huesa C, McKee MD, Farquharson C and Millán JL: Loss of skeletal mineralization by the simultaneous ablation of PHOSPHO1 and alkaline phosphatase function: A unified model of the mechanisms of initiation of skeletal calcification. J Bone Miner Res. 26:286–297. 2011. View Article : Google Scholar

23 

Narisawa S, Harmey D, Yadav MC, O’Neill WC, Hoylaerts MF and Millán JL: Novel inhibitors of alkaline phosphatase suppress vascular smooth muscle cell calcification. J Bone Miner Res. 22:1700–1710. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Sakamoto M, Hosoda Y, Kojimahara K, Yamazaki T and Yoshimura Y: Arthritis and ankylosis in twy mice with hereditary multiple osteochondral lesions: With special reference to calcium deposition. Pathol Int. 44:420–427. 1994. View Article : Google Scholar : PubMed/NCBI

25 

Okawa A, Goto S and Moriya H: Calcitonin simultaneously regulates both periosteal hyperostosis and trabecular osteopenia in the spinal hyperostotic mouse (twy/twy) in vivo. Calcif Tissue Int. 64:239–247. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Okawa A, Nakamura I, Goto S, Moriya H, Nakamura Y and Ikegawa S: Mutation in Npps in a mouse model of ossification of the posterior longitudinal ligament of the spine. Nat Genet. 19:271–273. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Baba H, Furusawa N, Fukuda M, Maezawa Y, Imura S, Kawahara N, Nakahashi K and Tomita K: Potential role of streptozotocin in enhancing ossification of the posterior longitudinal ligament of the cervical spine in the hereditary spinal hyperostotic mouse (twy/twy). Eur J Histochem. 41:191–202. 1997.PubMed/NCBI

28 

Furusawa N, Baba H, Imura S and Fukuda M: Characteristics and mechanism of the ossification of posterior longitudinal ligament in the tip-toe walking Yoshimura (twy) mouse. Eur J Histochem. 40:199–210. 1996.PubMed/NCBI

29 

Sali A, Favaloro J, Terkeltaub R and Goding J: Germline deletion of the nucleoside triphosphate pyrophosphohydrolase (NTPPPH) plasma cell membrane glycoprotein-1 (PC-1) produces abnormal calcification of periarticular tissues. Ecto-ATPases and Related Ectoenzymes. Vanduffel L and Lemmems R: Shaker Publishing BV; Maastricht, The Netherlands: pp. 267–282. 1999

30 

Harmey D, Hessle L, Narisawa S, Johnson KA, Terkeltaub R and Millán JL: Concerted regulation of inorganic pyrophosphate and osteopontin by akp2, enpp1, and ank: An integrated model of the pathogenesis of mineralization disorders. Am J Pathol. 164:1199–1209. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Anderson HC, Harmey D, Camacho NP, Garimella R, Sipe JB, Tague S, Bi X, Johnson K, Terkeltaub R and Millán JL: Sustained osteomalacia of long bones despite major improvement in other hypophosphatasia-related mineral deficits in tissue nonspecific alkaline phosphatase/nucleotide pyrophosphatase phosphodi-esterase 1 double-deficient mice. Am J Pathol. 166:1711–1720. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Johnson K, Goding J, Van Etten D, Sali A, Hu SI, Farley D, Krug H, Hessle L, Millán JL and Terkeltaub R: Linked deficiencies in extracellular PPi and osteopontin mediate pathologic calcification associated with defective PC-1 and ANK expression. J Bone Miner Res. 18:994–1004. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Russell RG: Bisphosphonates: From bench to bedside. Ann NY Acad Sci. 1068:367–401. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Orriss IR, Key ML, Colston KW and Arnett TR: Inhibition of osteoblast function in vitro by aminobisphosphonates. J Cell Biochem. 106:109–118. 2009. View Article : Google Scholar

35 

Idris AI, Rojas J, Greig IR, Van’t Hof RJ and Ralston SH: Aminobisphosphonates cause osteoblast apoptosis and inhibit bone nodule formation in vitro. Calcif Tissue Int. 82:191–201. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Iwata K, Li J, Follet H, Phipps RJ and Burr DB: Bisphosphonates suppress periosteal osteoblast activity independently of resorption in rat femur and tibia. Bone. 39:1053–1058. 2006. View Article : Google Scholar : PubMed/NCBI

37 

Tobias JH, Chow JW and Chambers TJ: 3-Amino-1-hydroxypropylidine-1-bisphosphonate (AHPrBP) suppresses not only the induction of new, but also the persistence of existing bone-forming surfaces in rat cancellous bone. Bone. 14:619–623. 1993. View Article : Google Scholar : PubMed/NCBI

38 

Rutsch F, Böyer P, Nitschke Y, Ruf N, Lorenz-Depierieux B, Wittkampf T, Weissen-Plenz G, Fischer RJ, Mughal Z, Gregory JW, et al: GACI Study Group: Hypophosphatemia, hyperphosphaturia, and bisphosphonate treatment are associated with survival beyond infancy in generalized arterial calcification of infancy. Circ Cardiovasc Genet. 1:133–140. 2008. View Article : Google Scholar

39 

Chong CR and Hutchins GM: Idiopathic infantile arterial calcification: The spectrum of clinical presentations. Pediatr Dev Pathol. 11:405–415. 2008. View Article : Google Scholar

40 

Lomashvili KA, Monier-Faugere MC, Wang X, Malluche HH and O’Neill WC: Effect of bisphosphonates on vascular calcification and bone metabolism in experimental renal failure. Kidney Int. 75:617–625. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Otero JE, Gottesman GS, McAlister WH, Mumm S, Madson KL, Kiffer-Moreira T, Sheen C, Millán JL, Ericson KL and Whyte MP: Severe skeletal toxicity from protracted etidronate therapy for generalized arterial calcification of infancy. J Bone Miner Res. 28:419–430. 2013. View Article : Google Scholar

42 

Huesa C, Zhu D, Glover JD, Ferron M, Karsenty G, Milne EM, Millan JL, Ahmed SF, Farquharson C, Morton NM, et al: Deficiency of the bone mineralization inhibitor NPP1 protects mice against obesity and diabetes. Dis Model Mech. 7:1341–1350. 2014. View Article : Google Scholar : PubMed/NCBI

43 

Sugiyama T, Meakin LB, Galea GL, Jackson BF, Lanyon LE, Ebetino FH, Russell RG and Price JS: Risedronate does not reduce mechanical loading-related increases in cortical and trabecular bone mass in mice. Bone. 49:133–139. 2011. View Article : Google Scholar : PubMed/NCBI

44 

Huesa C, Millán JL, van’t Hof RJ and MacRae VE: A new method for the quantification of aortic calcification by three-dimensional micro-computed tomography. Int J Mol Med. 32:1047–1050. 2013.PubMed/NCBI

45 

Huesa C, Yadav MC, Finnilä MA, Goodyear SR, Robins SP, Tanner KE, Aspden RM, Millán JL and Farquharson C: PHOSPHO1 is essential for mechanically competent mineralization and the avoidance of spontaneous fractures. Bone. 48:1066–1074. 2011. View Article : Google Scholar : PubMed/NCBI

46 

Hildebrand T and Ruegsegger P: A new method for the model-independent assessment of thickness in three-dimensional images. J Microscopy (Oxford). 185:67–75. 1997. View Article : Google Scholar

47 

Li Q and Uitto J: Mineralization/anti-mineralization networks in the skin and vascular connective tissues. Am J Pathol. 183:10–18. 2013. View Article : Google Scholar : PubMed/NCBI

48 

Black DM, Greenspan SL, Ensrud KE, Palermo L, McGowan JA, Lang TF, Garnero P, Bouxsein ML, Bilezikian JP and Rosen CJ: PaTH Study Investigators: The effects of parathyroid hormone and alendronate alone or in combination in postmenopausal osteoporosis. N Engl J Med. 349:1207–1215. 2003. View Article : Google Scholar : PubMed/NCBI

49 

Fleisch H, Russell RG and Straumann F: Effect of pyrophosphate on hydroxyapatite and its implications in calcium homeostasis. Nature. 212:901–903. 1966. View Article : Google Scholar : PubMed/NCBI

50 

Felix R, Herrmann W and Fleisch H: Stimulation of precipitation of calcium phosphate by matrix vesicles. Biochem J. 170:681–691. 1978.PubMed/NCBI

51 

Thiaville A, Smets A, Clercx A and Perlmutter N: Idiopathic infantile arterial calcification: A surviving patient with renal artery stenosis. Pediatr Radiol. 24:506–508. 1994. View Article : Google Scholar : PubMed/NCBI

52 

Van Dyck M, Proesmans W, Van Hollebeke E, Marchal G and Moerman P: Idiopathic infantile arterial calcification with cardiac, renal and central nervous system involvement. Eur J Pediatr. 148:374–377. 1989. View Article : Google Scholar : PubMed/NCBI

53 

Thomas T, Lafage MH and Alexandre C: Atypical osteomalacia after 2 year etidronate intermittent cyclic administration in osteoporosis. J Rheumatol. 22:2183–2185. 1995.PubMed/NCBI

54 

Silverman SL, Hurvitz EA, Nelson VS and Chiodo A: Rachitic syndrome after disodium etidronate therapy in an adolescent. Arch Phys Med Rehabil. 75:118–120. 1994.PubMed/NCBI

55 

Russell RG, Smith R, Preston C, Walton RJ and Woods CG: Diphosphonates in Paget’s disease. Lancet. 1:894–898. 1974.PubMed/NCBI

56 

Smith R, Russell RG and Woods CG: Myositis ossificans progressiva. Clinical features of eight patients and their response to treatment. J Bone Joint Surg Br. 58:48–57. 1976.PubMed/NCBI

57 

Kim S, Seiryu M, Okada S, Kuroishi T, Takano-Yamamoto T, Sugawara S and Endo Y: Analgesic effects of the non-nitrogen-containing bisphosphonates etidronate and clodronate, independent of anti-resorptive effects on bone. Eur J Pharmacol. 699:14–22. 2013. View Article : Google Scholar

58 

Li Q, Sundberg JP, Levine MA, Terry SF and Uitto J: The effects of bisphosphonates on ectopic soft tissue mineralization caused by mutations in the ABCC6gene. Cell Cycle. 14:1082–1089. 2015. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Huesa C, Staines KA, Millán JL and MacRae VE: Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy. Int J Mol Med 36: 159-165, 2015.
APA
Huesa, C., Staines, K.A., Millán, J.L., & MacRae, V.E. (2015). Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy. International Journal of Molecular Medicine, 36, 159-165. https://doi.org/10.3892/ijmm.2015.2212
MLA
Huesa, C., Staines, K. A., Millán, J. L., MacRae, V. E."Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy". International Journal of Molecular Medicine 36.1 (2015): 159-165.
Chicago
Huesa, C., Staines, K. A., Millán, J. L., MacRae, V. E."Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy". International Journal of Molecular Medicine 36, no. 1 (2015): 159-165. https://doi.org/10.3892/ijmm.2015.2212
Copy and paste a formatted citation
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Spandidos Publications style
Huesa C, Staines KA, Millán JL and MacRae VE: Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy. Int J Mol Med 36: 159-165, 2015.
APA
Huesa, C., Staines, K.A., Millán, J.L., & MacRae, V.E. (2015). Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy. International Journal of Molecular Medicine, 36, 159-165. https://doi.org/10.3892/ijmm.2015.2212
MLA
Huesa, C., Staines, K. A., Millán, J. L., MacRae, V. E."Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy". International Journal of Molecular Medicine 36.1 (2015): 159-165.
Chicago
Huesa, C., Staines, K. A., Millán, J. L., MacRae, V. E."Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy". International Journal of Molecular Medicine 36, no. 1 (2015): 159-165. https://doi.org/10.3892/ijmm.2015.2212
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