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Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro

  • Authors:
    • Xuexiao Ma
    • Yazhou Lin
    • Kun Yang
    • Bin Yue
    • Hongfei Xiang
    • Bohua Chen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The Affiliated Hospital of Qingdao University Medical College, Qingdao University, Qingdao, Shandong 266003, P.R. China, Medical Research Center, The Affiliated Hospital of Qingdao University Medical College, Qingdao University, Qingdao, Shandong 266003, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 186-194
    |
    Published online on: May 26, 2015
       https://doi.org/10.3892/ijmm.2015.2225
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Abstract

Lower back pain is a common concern, and 40% of all cases involve the degeneration of the intervertebral disc (IVD). However, the excessive apoptosis of disc cells plays an important role in IVD degeneration, particularly in the nucleus pulposus (NP). Thus, anti-apoptotic gene therapy to attenuate or reverse the degenerative process within the NP is being developed. Survivin is a unique inhibitor of apoptosis (IAP) and has been extensively investigated in cancer cells. However, little is known of the effects of survivin transfection on NP cells derived from degenerative human disc. In this study, we aimed to investigate the effects of lentivirus (LV)‑mediated survivin transfection on the morphology and apoptosis of NP cells derived from degenerative human disc in vitro. NP cells were transfected with LV‑mediated survivin. Subsequently, cell morphology was observed and the survivin mRNA expression levels were measured by RT‑qPCR. Apoptosis was analyzed by flow cytometry and by measuring caspase‑3 activity. The results revealed that the morphology of the NP cells derived from degenerative human disc transfected with LV‑mediated survivin was significantly altered as evidenced by cytomorphosis, the reduction of the cytoplasm and cell shrinkage. Following transfection, survivin gene expression significantly increased in the transfected cells and subsequent generation cells; however, no significant differences in the cell apoptotic rate and caspase‑3 activity were observed. We found that transfection of the survivin gene into NP cells led to the stable expression of survivin and induced marked changes in cell morphology. Furthermore, no significant anti-apoptotic effects were observed following LV‑mediated survivin transfection. Overall, our findings demonstrate that LV carrying surviving may be used to successfully enforce the expression of survivin in NP cells. However, cell morphology was evidently altered, whereas the apoptotic rate did not decrease. Comprehensive studies on the feasibility of using survivin in gene therapy in an aim to attenuate disc degeneration are warranted. Further research on the mechanisms responsible for the changes in cell morphology and cell function are also required.
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Copy and paste a formatted citation
Spandidos Publications style
Ma X, Lin Y, Yang K, Yue B, Xiang H and Chen B: Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro. Int J Mol Med 36: 186-194, 2015.
APA
Ma, X., Lin, Y., Yang, K., Yue, B., Xiang, H., & Chen, B. (2015). Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro. International Journal of Molecular Medicine, 36, 186-194. https://doi.org/10.3892/ijmm.2015.2225
MLA
Ma, X., Lin, Y., Yang, K., Yue, B., Xiang, H., Chen, B."Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro". International Journal of Molecular Medicine 36.1 (2015): 186-194.
Chicago
Ma, X., Lin, Y., Yang, K., Yue, B., Xiang, H., Chen, B."Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro". International Journal of Molecular Medicine 36, no. 1 (2015): 186-194. https://doi.org/10.3892/ijmm.2015.2225
Copy and paste a formatted citation
x
Spandidos Publications style
Ma X, Lin Y, Yang K, Yue B, Xiang H and Chen B: Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro. Int J Mol Med 36: 186-194, 2015.
APA
Ma, X., Lin, Y., Yang, K., Yue, B., Xiang, H., & Chen, B. (2015). Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro. International Journal of Molecular Medicine, 36, 186-194. https://doi.org/10.3892/ijmm.2015.2225
MLA
Ma, X., Lin, Y., Yang, K., Yue, B., Xiang, H., Chen, B."Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro". International Journal of Molecular Medicine 36.1 (2015): 186-194.
Chicago
Ma, X., Lin, Y., Yang, K., Yue, B., Xiang, H., Chen, B."Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro". International Journal of Molecular Medicine 36, no. 1 (2015): 186-194. https://doi.org/10.3892/ijmm.2015.2225
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