Interaction of AIM with insulin-like growth factor-binding protein-4

  • Authors:
    • Qiang You
    • Yan Wu
    • Nannan Yao
    • Guannan Shen
    • Ying Zhang
    • Liangguo Xu
    • Guiying Li
    • Cynthia Ju
  • View Affiliations

  • Published online on: June 26, 2015     https://doi.org/10.3892/ijmm.2015.2262
  • Pages: 833-838
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Abstract

Apoptosis inhibitor of macrophages (AIM/cluster of differentiation 5 antigen-like/soluble protein α) has been shown to inhibit cellular apoptosis; however, the underlying molecular mechanism has not been elucidated. Using yeast two‑hybrid screening, the present study uncovered that AIM binds to insulin‑like growth factor binding protein‑4 (IGFBP‑4). AIM interaction with IGFBP‑4, as well as IGFBP‑2 and ‑3, but not with IGFBP‑1, ‑5 and ‑6, was further confirmed by co‑immunoprecipitation (co‑IP) using 293 cells. The binding activity and affinity between AIM and IGFBP‑4 in vitro were analyzed by co‑IP and biolayer interferometry. Serum depletion‑induced cellular apoptosis was attenuated by insulin‑like growth factor‑I (IGF‑I), and this effect was abrogated by IGFBP‑4. Of note, in the presence of AIM, the inhibitory effect of IGFBP‑4 on the anti‑apoptosis function of IGF‑I was attenuated, possibly through binding of AIM with IGFBP‑4. In conclusion, to the best of our knowledge, the present study provides the first evidence that AIM binds to IGFBP‑2, ‑3 and ‑4. The data suggest that this interaction may contribute to the mechanism of AIM-mediated anti-apoptosis function.
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September-2015
Volume 36 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
You Q, Wu Y, Yao N, Shen G, Zhang Y, Xu L, Li G and Ju C: Interaction of AIM with insulin-like growth factor-binding protein-4. Int J Mol Med 36: 833-838, 2015
APA
You, Q., Wu, Y., Yao, N., Shen, G., Zhang, Y., Xu, L. ... Ju, C. (2015). Interaction of AIM with insulin-like growth factor-binding protein-4. International Journal of Molecular Medicine, 36, 833-838. https://doi.org/10.3892/ijmm.2015.2262
MLA
You, Q., Wu, Y., Yao, N., Shen, G., Zhang, Y., Xu, L., Li, G., Ju, C."Interaction of AIM with insulin-like growth factor-binding protein-4". International Journal of Molecular Medicine 36.3 (2015): 833-838.
Chicago
You, Q., Wu, Y., Yao, N., Shen, G., Zhang, Y., Xu, L., Li, G., Ju, C."Interaction of AIM with insulin-like growth factor-binding protein-4". International Journal of Molecular Medicine 36, no. 3 (2015): 833-838. https://doi.org/10.3892/ijmm.2015.2262