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Article

YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways

  • Authors:
    • Sung Hwa Paeng
    • Won Sun Park
    • Won-Kyo Jung
    • Dae-Sung Lee
    • Gi-Young Kim
    • Yung Hyun Choi
    • Su-Kil Seo
    • Won Hee Jang
    • Jung Sik Choi
    • Young-Min Lee
    • Saegwang Park
    • Il-Whan Choi
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Busan Paik Hospital, Inje University College of Medicine, Busan, Republic of Korea, Department of Physiology, Kangwon National University School of Medicine, Chuncheon, Gangwon, Republic of Korea, Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, Republic of Korea, Marine Biodiversity Institute of Korea, Seocheon, Chungcheongnam-do, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju, Republic of Korea, Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan, Republic of Korea, Department of Microbiology, Inje University College of Medicine, Busan, Republic of Korea, Department of Biochemistry, Inje University College of Medicine, Busan, Republic of Korea, Department of Internal Medicine, Busan Paik Hospital, Inje University College of Medicine, Busan, Republic of Korea
  • Pages: 808-816
    |
    Published online on: June 30, 2015
       https://doi.org/10.3892/ijmm.2015.2266
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Abstract

YCG063 is known as an inhibitor of reactive oxygen species (ROS); however, its intracellular mechanisms of action remain poorly understood. In the present study, we investigated the effects of YCG063 on the inflammatory response of Pseudomonas aeruginosa lipopolysaccharide (PA-LPS)‑stimulated human retinal pigment epithelial cells (RPE cells). Human adult RPE cells (ARPE‑19) were stimulated with PA-LPS. We then investigated the LPS-induced expression of several inflammatory mediators, such as interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1 (MCP-1) and intracellular adhesion molecule-1 (ICAM-1) in the ARPE-19 cells. We performed an enzyme-linked immunosorbent assay (ELISA), western blot analysis, electrophoretic mobility shift assay (EMSA) and fluorescence-activated cell sorting (FACS) to elucidate the mechanisms involved in the anti-inflammatory effects of YCG063 in the PA-LPS-stimulated cells. The results revealed that treatment with YCG063 significantly inhibited the levels of IL-6, IL-8, MCP-1 and ICAM-1 in the PA-LPS-stimulated ARPE-19 cells. YCG063 also markedly inhibited the phosphorylation of AKT in the PA‑LPS-stimulated cells. In addition, the activation of nuclear factor-κB (NF-κB) was also attenuated folllowing treatment with YCG063. ROS were not generated in the PA-LPS-stimulated cells. In conclusion, our data indicate that YCG063 may prove to be a potential protective agent against inflammation, possibly through the downregulation of Toll‑like receptor 2 (TLR2) and the AKT-dependent NF-κB activation pathway in PA-LPS-stimulated ARPE-19 cells. Furthermore, this anti-inflammatory activity occurred through ROS-independent signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Paeng SH, Park WS, Jung W, Lee D, Kim G, Choi YH, Seo S, Jang WH, Choi JS, Lee Y, Lee Y, et al: YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways. Int J Mol Med 36: 808-816, 2015.
APA
Paeng, S.H., Park, W.S., Jung, W., Lee, D., Kim, G., Choi, Y.H. ... Choi, I. (2015). YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways. International Journal of Molecular Medicine, 36, 808-816. https://doi.org/10.3892/ijmm.2015.2266
MLA
Paeng, S. H., Park, W. S., Jung, W., Lee, D., Kim, G., Choi, Y. H., Seo, S., Jang, W. H., Choi, J. S., Lee, Y., Park, S., Choi, I."YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways". International Journal of Molecular Medicine 36.3 (2015): 808-816.
Chicago
Paeng, S. H., Park, W. S., Jung, W., Lee, D., Kim, G., Choi, Y. H., Seo, S., Jang, W. H., Choi, J. S., Lee, Y., Park, S., Choi, I."YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways". International Journal of Molecular Medicine 36, no. 3 (2015): 808-816. https://doi.org/10.3892/ijmm.2015.2266
Copy and paste a formatted citation
x
Spandidos Publications style
Paeng SH, Park WS, Jung W, Lee D, Kim G, Choi YH, Seo S, Jang WH, Choi JS, Lee Y, Lee Y, et al: YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways. Int J Mol Med 36: 808-816, 2015.
APA
Paeng, S.H., Park, W.S., Jung, W., Lee, D., Kim, G., Choi, Y.H. ... Choi, I. (2015). YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways. International Journal of Molecular Medicine, 36, 808-816. https://doi.org/10.3892/ijmm.2015.2266
MLA
Paeng, S. H., Park, W. S., Jung, W., Lee, D., Kim, G., Choi, Y. H., Seo, S., Jang, W. H., Choi, J. S., Lee, Y., Park, S., Choi, I."YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways". International Journal of Molecular Medicine 36.3 (2015): 808-816.
Chicago
Paeng, S. H., Park, W. S., Jung, W., Lee, D., Kim, G., Choi, Y. H., Seo, S., Jang, W. H., Choi, J. S., Lee, Y., Park, S., Choi, I."YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways". International Journal of Molecular Medicine 36, no. 3 (2015): 808-816. https://doi.org/10.3892/ijmm.2015.2266
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