Open Access

5-Azacitidine induces demethylation of PTPL1 and inhibits growth in non-Hodgkin lymphoma

  • Authors:
    • Wenming Wang
    • Jing Wang
    • Min Li
    • Jianming Ying
    • Hongmei Jing
  • View Affiliations

  • Published online on: July 1, 2015     https://doi.org/10.3892/ijmm.2015.2269
  • Pages: 698-704
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Non-Hodgkin lymphoma (NHL) consists of various lymphoid malignancies with a diverse clinical pathology and biological characteristics. Methylation of cytosine residues by DNA methyltransferases at CpG dinucleotides in the promoter region of the genes is a major epigenetic modification in mammalian genomes that can have profound effects on gene expression. The PTPL1 methylation pattern was screened by methylation‑specific polymerase chain reaction (MSP) in 7 lymphoma‑derived cell lines and in 47 samples of diffuse large B cell lymphoma (DLBCL). The PTPL1 gene was hypermethylated in the CA46, Raji, Jurkat and DB cell lines; however, it was unmethylated in the Hut78, Maver and Z138 cell lines. The expression of PTPL1 mRNA was re‑inducible by 5‑azacytidine (5‑Aza), an agent of DNA demethylation. The methylations were detected in 59.6% of DLBCL versus 6.3% in reactive lymph node proliferation. Therefore, the present data showed that PTPL1 was epigenetically regulated in NHL suggesting an involvement of the PTPL1 tumor‑suppressor genes in NHL, and highlights 5-Aza as a potential therapeutic candidate for NHL.
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September-2015
Volume 36 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang W, Wang J, Li M, Ying J and Jing H: 5-Azacitidine induces demethylation of PTPL1 and inhibits growth in non-Hodgkin lymphoma. Int J Mol Med 36: 698-704, 2015
APA
Wang, W., Wang, J., Li, M., Ying, J., & Jing, H. (2015). 5-Azacitidine induces demethylation of PTPL1 and inhibits growth in non-Hodgkin lymphoma. International Journal of Molecular Medicine, 36, 698-704. https://doi.org/10.3892/ijmm.2015.2269
MLA
Wang, W., Wang, J., Li, M., Ying, J., Jing, H."5-Azacitidine induces demethylation of PTPL1 and inhibits growth in non-Hodgkin lymphoma". International Journal of Molecular Medicine 36.3 (2015): 698-704.
Chicago
Wang, W., Wang, J., Li, M., Ying, J., Jing, H."5-Azacitidine induces demethylation of PTPL1 and inhibits growth in non-Hodgkin lymphoma". International Journal of Molecular Medicine 36, no. 3 (2015): 698-704. https://doi.org/10.3892/ijmm.2015.2269