Open Access

Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro

  • Authors:
    • Yanyan Bian
    • Wenwei Qian
    • Hongling Li
    • Robert Chunhua Zhao
    • Wang Xing Shan
    • Xisheng Weng
  • View Affiliations

  • Published online on: July 6, 2015     https://doi.org/10.3892/ijmm.2015.2273
  • Pages: 678-684
  • Copyright: © Bian et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Avascular necrosis of the femoral head (ANFH) occurs following exposure to corticosteroids, and the proliferative capacity of the mesenchymal stem cells (MSCs) belonging to ANFH was reduced. The previous studies indicate that microRNA (miRNA) has an important regulatory role during proliferation and osteogenic differentiation of MSCs. Therefore, MSCs were obtained from healthy adults, and were cultured and osteogenically‑induced by different dexamethasone concentrations. The proliferation and osteogenic differentiation capacities were examined through observing cellular morphology, alkaline phosphatase and alizarin red; miRNA expression was investigated using an miRNA gene chip and miRNA of differential expressions were retrieved through a database to analyze its regulatory effect. Dexamethasone at a concentration of 10‑7 mol/l induced the proliferation and osteogenic differentiation of MSCs and resulted in evident miRNA expression profile changes. In total, 11 miRNAs were upregulated at 10‑7 mol/l while 6 were downregulated, and partial miRNA was identified to participate in the regulation of cell proliferation and cell apoptosis, MSC osteogenic differentiation, lipid metabolism and other processes.
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September-2015
Volume 36 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Bian Y, Qian W, Li H, Zhao RC, Shan WX and Weng X: Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro. Int J Mol Med 36: 678-684, 2015
APA
Bian, Y., Qian, W., Li, H., Zhao, R.C., Shan, W.X., & Weng, X. (2015). Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro. International Journal of Molecular Medicine, 36, 678-684. https://doi.org/10.3892/ijmm.2015.2273
MLA
Bian, Y., Qian, W., Li, H., Zhao, R. C., Shan, W. X., Weng, X."Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro". International Journal of Molecular Medicine 36.3 (2015): 678-684.
Chicago
Bian, Y., Qian, W., Li, H., Zhao, R. C., Shan, W. X., Weng, X."Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro". International Journal of Molecular Medicine 36, no. 3 (2015): 678-684. https://doi.org/10.3892/ijmm.2015.2273