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International Journal of Molecular Medicine
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Article

Aplasia Ras homologue member Ⅰ overexpression inhibits tumor growth and induces apoptosis through inhibition of PI3K/Akt survival pathways in human osteosarcoma MG-63 cells in culture

  • Authors:
    • Kaishan Ye
    • Shuanke Wang
    • Yong Yang
    • Xuewen Kang
    • Jing Wang
    • Hua Han
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Lanzhou University Second Hospital, Lanzhou, Gansu 730030, P.R. China
  • Pages: 776-782
    |
    Published online on: July 8, 2015
       https://doi.org/10.3892/ijmm.2015.2278
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Abstract

Aplasia Ras homologue member Ⅰ (ARHI), an imprinted tumor-suppressor gene, is downregulated in various types of cancer. However, the expression, function and specific mechanisms of ARHI in human osteosarcoma (OS) cells remain unclear. The aim of the present study was to assess the effect of ARHI on OS cell proliferation and apoptosis and its associated mechanism. In the study, ARHI mRNA and protein levels were markedly downregulated in OS cells compared with the human osteoblast precursor cell line hFOB1.19. By generating stable transfectants, ARHI was overexpressed in OS cells that had low levels of ARHI. Overexpression of ARHI inhibited cell viability and proliferation and induced apoptosis. However, caspase‑3 activity was not changed by ARHI overexpression. In addition, phosphorylated Akt protein expression decreased in the ARHI overexpression group compared to that in the control vector group. The knockdown of ARHI also resulted in the promotion of cell proliferation and the attenuation of apoptosis in MG‑63 cells. Additionally, ARHI silencing increased the level of p‑Akt. The present results indicate that ARHI inhibits OS cell proliferation and may have a key role in the development of OS.
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Copy and paste a formatted citation
Spandidos Publications style
Ye K, Wang S, Yang Y, Kang X, Wang J and Han H: Aplasia Ras homologue member Ⅰ overexpression inhibits tumor growth and induces apoptosis through inhibition of PI3K/Akt survival pathways in human osteosarcoma MG-63 cells in culture. Int J Mol Med 36: 776-782, 2015.
APA
Ye, K., Wang, S., Yang, Y., Kang, X., Wang, J., & Han, H. (2015). Aplasia Ras homologue member Ⅰ overexpression inhibits tumor growth and induces apoptosis through inhibition of PI3K/Akt survival pathways in human osteosarcoma MG-63 cells in culture. International Journal of Molecular Medicine, 36, 776-782. https://doi.org/10.3892/ijmm.2015.2278
MLA
Ye, K., Wang, S., Yang, Y., Kang, X., Wang, J., Han, H."Aplasia Ras homologue member Ⅰ overexpression inhibits tumor growth and induces apoptosis through inhibition of PI3K/Akt survival pathways in human osteosarcoma MG-63 cells in culture". International Journal of Molecular Medicine 36.3 (2015): 776-782.
Chicago
Ye, K., Wang, S., Yang, Y., Kang, X., Wang, J., Han, H."Aplasia Ras homologue member Ⅰ overexpression inhibits tumor growth and induces apoptosis through inhibition of PI3K/Akt survival pathways in human osteosarcoma MG-63 cells in culture". International Journal of Molecular Medicine 36, no. 3 (2015): 776-782. https://doi.org/10.3892/ijmm.2015.2278
Copy and paste a formatted citation
x
Spandidos Publications style
Ye K, Wang S, Yang Y, Kang X, Wang J and Han H: Aplasia Ras homologue member Ⅰ overexpression inhibits tumor growth and induces apoptosis through inhibition of PI3K/Akt survival pathways in human osteosarcoma MG-63 cells in culture. Int J Mol Med 36: 776-782, 2015.
APA
Ye, K., Wang, S., Yang, Y., Kang, X., Wang, J., & Han, H. (2015). Aplasia Ras homologue member Ⅰ overexpression inhibits tumor growth and induces apoptosis through inhibition of PI3K/Akt survival pathways in human osteosarcoma MG-63 cells in culture. International Journal of Molecular Medicine, 36, 776-782. https://doi.org/10.3892/ijmm.2015.2278
MLA
Ye, K., Wang, S., Yang, Y., Kang, X., Wang, J., Han, H."Aplasia Ras homologue member Ⅰ overexpression inhibits tumor growth and induces apoptosis through inhibition of PI3K/Akt survival pathways in human osteosarcoma MG-63 cells in culture". International Journal of Molecular Medicine 36.3 (2015): 776-782.
Chicago
Ye, K., Wang, S., Yang, Y., Kang, X., Wang, J., Han, H."Aplasia Ras homologue member Ⅰ overexpression inhibits tumor growth and induces apoptosis through inhibition of PI3K/Akt survival pathways in human osteosarcoma MG-63 cells in culture". International Journal of Molecular Medicine 36, no. 3 (2015): 776-782. https://doi.org/10.3892/ijmm.2015.2278
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