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Article

Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway

  • Authors:
    • Qiang Zhao
    • Xue-Yao Wang
    • Xiao-Xia Yu
    • Ying-Xian Zhai
    • Xu He
    • Shan Wu
    • Ying-Ai Shi
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Norman Bethune College of Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 857-864
    |
    Published online on: July 15, 2015
       https://doi.org/10.3892/ijmm.2015.2284
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Abstract

Multipotent mesenchymal stem cells (MSCs) are widely used as seed cells in studies of tissue engineering and regenerative medicine; however, their clinical application is limited due to replicative senescence. It has been demonstrated that telomerase expression extends the lifespan and maintains the bone-forming ability of MSCs; however, the detailed role and the underlying molecular mechanisms in MSCs remain largely unknown. In the present study, we found that senescence was associated with human telomerase reverse transcriptase (hTERT) expression, and telomere length and telomerase activity. We established a short interfering RNA (siRNA) targeting hTERT and a gene expression vector carrying hTERT and transfected these into the MSCs to investigate the detailed role and the underlying molecular mechanisms of action of hTERT in MSCs. We found that the downregulation of hTERT by siRNA markedly decreased telomere length and telomerase activity in the MSCs, whereas the overexpression of hTERT increased telomere length and telomerase activity in the MSCs. The downregulation of hTERT inhibited cell proliferation and promoted the senescence and apoptosis of MSCs, whereas the upregulation of hTERT increased cell proliferation and decreased the senescence and apoptosis of MSCs. Of note, we also found that the activation of the PI3K/AKT signaling pathway was mediated by hTERT and that blocking this pathway using LY294002 inhibited hTERT expression, induced senescence and decreased the proliferation of MSCs. These findings reveal a previously unknown regulatory mechanism of hTERT, indicating that hTERT mediates the senescence of MSCs through the PI3K/AKT signaling pathway.
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1 

Bayati V, Hashemitabar M, Gazor R, Nejatbakhsh R and Bijannejad D: Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study. Anat Cell Biol. 46:113–121. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Wakitani S, Saito T and Caplan AI: Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve. 18:1417–1426. 1995. View Article : Google Scholar : PubMed/NCBI

3 

Javazon EH, Colter DC, Schwarz EJ and Prockop DJ: Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells. Stem Cells. 19:219–225. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Beyer Nardi N and da Silva Meirelles L: Mesenchymal stem cells: isolation, in vitro expansion and characterization. Handb Exp Pharmacol. 174:249–282. 2006. View Article : Google Scholar

5 

Brighton CT and Hunt RM: Early histological and ultrastructural changes in medullary fracture callus. J Bone Joint Surg Am. 73:832–847. 1991.PubMed/NCBI

6 

Caplan AI: Mesenchymal stem cells. J Orthop Res. 9:641–650. 1991. View Article : Google Scholar : PubMed/NCBI

7 

Li J and Pei M: Cell senescence: A challenge in cartilage engineering and regeneration. Tissue Eng Part B Rev. 18:270–287. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Allsopp RC, Vaziri H, Patterson C, Goldstein S, Younglai EV, Futcher AB, Greider CW and Harley CB: Telomere length predicts replicative capacity of human fibroblasts. Proc Natl Acad Sci USA. 89:10114–10118. 1992. View Article : Google Scholar : PubMed/NCBI

9 

Broccoli D, Young JW and de Lange T: Telomerase activity in normal and malignant hematopoietic cells. Proc Natl Acad Sci USA. 92:9082–9086. 1995. View Article : Google Scholar : PubMed/NCBI

10 

Counter CM, Avilion AA, LeFeuvre CE, Stewart NG, Greider CW, Harley CB and Bacchetti S: Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J. 11:1921–1929. 1992.PubMed/NCBI

11 

Vaziri H and Benchimol S: Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr Biol. 8:279–282. 1998. View Article : Google Scholar : PubMed/NCBI

12 

Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S and Wright WE: Extension of life-span by introduction of telomerase into normal human cells. Science. 279:349–352. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Greider CW and Blackburn EH: A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature. 337:331–337. 1989. View Article : Google Scholar : PubMed/NCBI

14 

Feng J, Funk WD, Wang SS, Weinrich SL, Avilion AA, Chiu CP, Adams RR, Chang E, Allsopp RC, Yu J, et al: The RNA component of human telomerase. Science. 269:1236–1241. 1995. View Article : Google Scholar : PubMed/NCBI

15 

Kassem M, Abdallah BM, Yu Z, Ditzel N and Burns JS: The use of hTERT-immortalized cells in tissue engineering. Cytotechnology. 45:39–46. 2004. View Article : Google Scholar

16 

Tao Q, Lv B, Qiao B, Zheng CQ and Chen ZF: Immortalization of ameloblastoma cells via reactivation of telomerase function: Phenotypic and molecular characteristics. Oral Oncol. 45:e239–e244. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Morales CP, Holt SE, Ouellette M, Kaur KJ, Yan Y, Wilson KS, White MA, Wright WE and Shay JW: Absence of cancer-associated changes in human fibroblasts immortalized with telomerase. Nat Genet. 21:115–118. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Rajamani K, Lin YC, Wen TC, Hsieh J, Subeq YM, Liu JW, Lin PC, Harn HJ, Lin SZ and Chiou TW: The antisenescence effect of trans-cinnamaldehyde on adipose-derived stem cells. Cell Transplant. 24:493–507. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Yang J, Chang E, Cherry AM, Bangs CD, Oei Y, Bodnar A, Bronstein A, Chiu CP and Herron GS: Human endothelial cell life extension by telomerase expression. J Biol Chem. 274:26141–26148. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Bischoff DS, Makhijani NS and Yamaguchi DT: Constitutive expression of human telomerase enhances the proliferation potential of human mesenchymal stem cells. Biores Open Access. 1:273–279. 2012. View Article : Google Scholar

21 

Huang G, Zheng Q, Sun J, Guo C, Yang J, Chen R, Xu Y, Wang G, Shen D, Pan Z, et al: Stabilization of cellular properties and differentiation mutilpotential of human mesenchymal stem cells transduced with hTERT gene in a long-term culture. J Cell Biochem. 103:1256–1269. 2008. View Article : Google Scholar

22 

Kobune M, Kawano Y, Ito Y, Chiba H, Nakamura K, Tsuda H, Sasaki K, Dehari H, Uchida H, Honmou O, et al: Telomerized human multipotent mesenchymal cells can differentiate into hematopoietic and cobblestone area-supporting cells. Exp Hematol. 31:715–722. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Piper SL, Wang M, Yamamoto A, Malek F, Luu A, Kuo AC and Kim HT: Inducible immortality in hTERT-human mesenchymal stem cells. J Orthop Res. 30:1879–1885. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Moffatt-Jauregui CE, Robinson B, de Moya AV, Brockman RD, Roman AV, Cash MN, Culp DJ and Lamont RJ: Establishment and characterization of a telomerase immortalized human gingival epithelial cell line. J Periodontal Res. 48:713–721. 2013.PubMed/NCBI

25 

Yang YX, Miao ZC, Zhang HJ, Wang Y, Gao JX and Feng MF: Establishment and characterization of a human telomerase catalytic subunit-transduced fetal bone marrow-derived osteoblastic cell line. Differentiation. 75:24–34. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Scutt A and Bertram P: Bone marrow cells are targets for the anabolic actions of prostaglandin E2 on bone: induction of a transition from nonadherent to adherent osteoblast precursors. J Bone Miner Res. 10:474–487. 1995. View Article : Google Scholar : PubMed/NCBI

27 

Galderisi U, Helmbold H, Squillaro T, Alessio N, Komm N, Khadang B, Cipollaro M, Bohn W and Giordano A: In vitro senescence of rat mesenchymal stem cells is accompanied by downregulation of stemness-related and DNA damage repair genes. Stem Cells Dev. 18:1033–1042. 2009. View Article : Google Scholar

28 

Shi YA, Zhao Q, Zhang LH, Du W, Wang XY, He X, Wu S and Li YL: Knockdown of hTERT by siRNA inhibits cervical cancer cell growth in vitro and in vivo. Int J Oncol. 45:1216–1224. 2014.PubMed/NCBI

29 

Palm W and de Lange T: How shelterin protects mammalian telomeres. Annu Rev Genet. 42:301–334. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Follo MY, Manzoli L, Poli A, McCubrey JA and Cocco L: PLC and PI3K/Akt/mTOR signalling in disease and cancer. Adv Biol Regul. 57:10–16. 2015. View Article : Google Scholar

31 

Yoon KA, Cho HS, Shin HI and Cho JY: Differential regulation of CXCL5 by FGF2 in osteoblastic and endothelial niche cells supports hematopoietic stem cell migration. Stem Cells Dev. 21:3391–3402. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

33 

Stewart JA, Chaiken MF, Wang F and Price CM: Maintaining the end: Roles of telomere proteins in end-protection, telomere replication and length regulation. Mutat Res. 730:12–19. 2012. View Article : Google Scholar :

34 

Serakinci N, Graakjaer J and Kolvraa S: Telomere stability and telomerase in mesenchymal stem cells. Biochimie. 90:33–40. 2008. View Article : Google Scholar

35 

Fajkus J, Simícková M and Maláska J: Tiptoeing to chromosome tips: facts, promises and perils of today's human telomere biology. Philos Trans R Soc Lond B Biol Sci. 357:545–562. 2002. View Article : Google Scholar : PubMed/NCBI

36 

Dimitrova V and Arcaro A: Targeting the PI3K/AKT/mTOR signaling pathway in medulloblastoma. Curr Mol Med. 15:82–93. 2015. View Article : Google Scholar : PubMed/NCBI

37 

Chell JM and Brand AH: Nutrition-responsive glia control exit of neural stem cells from quiescence. Cell. 143:1161–1173. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Sewell GW, Marks DJ and Segal AW: The immunopathogenesis of Crohn's disease: a three-stage model. Curr Opin Immunol. 21:506–513. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Kang SS, Kwon T, Kwon DY and Do SI: Akt protein kinase enhances human telomerase activity through phosphorylation of telomerase reverse transcriptase subunit. J Biol Chem. 274:13085–13090. 1999. View Article : Google Scholar : PubMed/NCBI

40 

Kawagoe J, Ohmichi M, Takahashi T, Ohshima C, Mabuchi S, Takahashi K, Igarashi H, Mori-Abe A, Saitoh M, Du B, et al: Raloxifene inhibits estrogen-induced up-regulation of telomerase activity in a human breast cancer cell line. J Biol Chem. 278:43363–43372. 2003. View Article : Google Scholar : PubMed/NCBI

41 

Kimura A, Ohmichi M, Kawagoe J, Kyo S, Mabuchi S, Takahashi T, Ohshima C, Arimoto-Ishida E, Nishio Y, Inoue M, et al: Induction of hTERT expression and phosphorylation by estrogen via Akt cascade in human ovarian cancer cell lines. Oncogene. 23:4505–4515. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao Q, Wang X, Yu X, Zhai Y, He X, Wu S and Shi Y: Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway. Int J Mol Med 36: 857-864, 2015.
APA
Zhao, Q., Wang, X., Yu, X., Zhai, Y., He, X., Wu, S., & Shi, Y. (2015). Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway. International Journal of Molecular Medicine, 36, 857-864. https://doi.org/10.3892/ijmm.2015.2284
MLA
Zhao, Q., Wang, X., Yu, X., Zhai, Y., He, X., Wu, S., Shi, Y."Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway". International Journal of Molecular Medicine 36.3 (2015): 857-864.
Chicago
Zhao, Q., Wang, X., Yu, X., Zhai, Y., He, X., Wu, S., Shi, Y."Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway". International Journal of Molecular Medicine 36, no. 3 (2015): 857-864. https://doi.org/10.3892/ijmm.2015.2284
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Q, Wang X, Yu X, Zhai Y, He X, Wu S and Shi Y: Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway. Int J Mol Med 36: 857-864, 2015.
APA
Zhao, Q., Wang, X., Yu, X., Zhai, Y., He, X., Wu, S., & Shi, Y. (2015). Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway. International Journal of Molecular Medicine, 36, 857-864. https://doi.org/10.3892/ijmm.2015.2284
MLA
Zhao, Q., Wang, X., Yu, X., Zhai, Y., He, X., Wu, S., Shi, Y."Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway". International Journal of Molecular Medicine 36.3 (2015): 857-864.
Chicago
Zhao, Q., Wang, X., Yu, X., Zhai, Y., He, X., Wu, S., Shi, Y."Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway". International Journal of Molecular Medicine 36, no. 3 (2015): 857-864. https://doi.org/10.3892/ijmm.2015.2284
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