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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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September-2015 Volume 36 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro

  • Authors:
    • Zengmei Lan
    • Zhaoyang Chong
    • Cong Liu
    • Danyang Feng
    • Dihai Fang
    • Weijin Zang
    • Jun Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China, Department of Cardiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 904-910
    |
    Published online on: July 20, 2015
       https://doi.org/10.3892/ijmm.2015.2289
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Abstract

Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies worldwide, and its incidence associated with viral infection has increased in recent years. Amantadine is a tricyclic symmetric amine that can effectively protect against the hepatitis C virus. However, its antitumor properties remain unclear. In the present study, the effects of amantadine on tumor cell viability, cell cycle regulation and apoptosis were investigated. The growth of HepG2 and SMMC‑7721 cells (HCC cell lines) was detected by an MTT assay. Flow cytometry was used to investigate cell cycle regulation and apoptosis. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were also performed to examine the expression of cell cycle‑ and apoptosis‑related genes and proteins, including cyclin E, cyclin D1, cyclin‑dependent kinase 2 (CDK2), B‑cell lymphoma 2 (Bcl‑2) and Bax. Our results demonstrated that amantadine markedly inhibited the proliferation of HepG2 and SMMC‑7721 cells in a dose‑ and time‑dependent manner and arrested the cell cycle at the G0/G1 phase. The levels of the cell cycle‑related genes and proteins (cyclin D1, cyclin E and CDK2) were reduced by amantadine, and apoptosis was significantly induced. Amantadine treatment also reduced Bcl‑2 and increased the Bax protein and mRNA levels. Additionally, Bcl‑2/Bax ratios were lower in the two HCC cell lines following amantadine treatment. Collectively, these results emphasize the role of amantadine in suppressing proliferation and inducing apoptosis in HCC cells, advocating its use as a novel tumor-suppressive therapeutic candidate.
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Copy and paste a formatted citation
Spandidos Publications style
Lan Z, Chong Z, Liu C, Feng D, Fang D, Zang W and Zhou J: Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro. Int J Mol Med 36: 904-910, 2015.
APA
Lan, Z., Chong, Z., Liu, C., Feng, D., Fang, D., Zang, W., & Zhou, J. (2015). Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro. International Journal of Molecular Medicine, 36, 904-910. https://doi.org/10.3892/ijmm.2015.2289
MLA
Lan, Z., Chong, Z., Liu, C., Feng, D., Fang, D., Zang, W., Zhou, J."Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro". International Journal of Molecular Medicine 36.3 (2015): 904-910.
Chicago
Lan, Z., Chong, Z., Liu, C., Feng, D., Fang, D., Zang, W., Zhou, J."Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro". International Journal of Molecular Medicine 36, no. 3 (2015): 904-910. https://doi.org/10.3892/ijmm.2015.2289
Copy and paste a formatted citation
x
Spandidos Publications style
Lan Z, Chong Z, Liu C, Feng D, Fang D, Zang W and Zhou J: Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro. Int J Mol Med 36: 904-910, 2015.
APA
Lan, Z., Chong, Z., Liu, C., Feng, D., Fang, D., Zang, W., & Zhou, J. (2015). Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro. International Journal of Molecular Medicine, 36, 904-910. https://doi.org/10.3892/ijmm.2015.2289
MLA
Lan, Z., Chong, Z., Liu, C., Feng, D., Fang, D., Zang, W., Zhou, J."Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro". International Journal of Molecular Medicine 36.3 (2015): 904-910.
Chicago
Lan, Z., Chong, Z., Liu, C., Feng, D., Fang, D., Zang, W., Zhou, J."Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro". International Journal of Molecular Medicine 36, no. 3 (2015): 904-910. https://doi.org/10.3892/ijmm.2015.2289
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