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International Journal of Molecular Medicine
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Article

Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in the chondrocytes of patients with osteoarthritis

  • Authors:
    • Chao Liu
    • Yongping Cao
    • Xin Yang
    • Pengcheng Shan
    • Heng Liu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China, Department of Orthopedics, Peking University First Hospital, Beijing 100034, P.R. China
  • Pages: 1081-1087
    |
    Published online on: July 29, 2015
       https://doi.org/10.3892/ijmm.2015.2295
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Abstract

The main pathogenic events in osteoarthritis (OA) include loss and abnormal remodeling of cartilage extracellular matrix. The present study aimed to evaluate the protective effect of tauroursodeoxycholic acid on chondrocyte apoptosis induced by endoplasmic reticulum (ER) stress. Articular cartilage tissues were collected from 18 patients who underwent total knee arthroplasty and were analyzed histologically. Subsequently, chondrocyte apoptosis was assessed by TUNEL. Quantitative polymerase chain reaction and western blot analysis were employed to evaluate gene and protein expression, respectively, of ER stress markers, including glucose‑regulated protein 78 (GRP78), growth arrest and DNA‑damage‑inducible gene 153 (GADD153) and caspase‑12 along with type II collagen. Chondrocytes obtained from osteoarthritis patients at different stages were cultured in three conditions including: No treatment (CON group), tunicamycin treatment to induce ER stress (ERS group) and tauroursodeoxycholic acid treatment after 4 h of tunicamycin (TDA group); and cell proliferation, apoptosis, function and ER stress level were assessed. Degradation of cartilage resulted in histological damage with more apoptotic cartilage cells observed. Of note, GRP78, GADD153 and caspase‑12 mRNA and protein expression increased gradually from grade I to III cartilage tissue, while type II collagen expression decreased. Tunicamycin induced ER stress, as shown by a high expression of ER stress markers, reduced cell proliferation, increased apoptosis and decreased synthesis of type II collagen. Notably, tauroursodeoxycholic acid treatment resulted in the improvement of tunicamycin‑induced ER stress. These results indicated that ER stress is highly involved in the tunicamycin‑induced apoptosis in chondrocytes, which can be prevented by tauroursodeoxycholic acid.
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View References

1 

Loeser RF: Aging and osteoarthritis: The role of chondrocyte senescence and aging changes in the cartilage matrix. Osteoarthritis Cartilage. 17:971–979. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Creamer P and Hochberg MC: Osteoarthritis. Lancet. 350:503–508. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Blanco FJ, Guitian R, Vázquez-Martul E, de Toro FJ and Galdo: Osteoarthritis chondrocytes die by apoptosis. A possible pathway for osteoarthritis pathology. Arthritis Rheum. 41:284–289. 1998. View Article : Google Scholar : PubMed/NCBI

4 

Hashimoto S, Ochs RL, Komiya S and Lotz M: Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis. Arthritis Rheum. 41:1632–1638. 1998. View Article : Google Scholar : PubMed/NCBI

5 

Kühn K, D'Lima DD, Hashimoto S and Lotz M: Cell death in cartilage. Osteoarthritis Cartilage. 12:1–16. 2004. View Article : Google Scholar

6 

Aigner T, Kurz B, Fukui N and Sandell L: Roles of chondrocytes in the pathogenesis of osteoarthritis. Curr Opin Rheumatol. 14:578–584. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Boyce M and Yuan J: Cellular response to endoplasmic reticulum stress: A matter of life or death. Cell Death Differ. 13:363–373. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Brostrom MA and Brostrom CO: Calcium dynamics and endoplasmic reticular function in the regulation of protein synthesis: Implications for cell growth and adaptability. Cell Calcium. 34:345–363. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Archer CW and Francis-West P: The chondrocyte. Int J Biochem Cell Biol. 35:401–404. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Yang L, Carlson SG, McBurney D and Horton WE Jr: Multiple signals induce endoplasmic reticulum stress in both primary and immortalized chondrocytes resulting in loss of differentiation, impaired cell growth, and apoptosis. J Biol Chem. 280:31156–31165. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Ruiz-Romero C, Carreira V, Rego I, Remeseiro S, López-Armada MJ and Blanco FJ: Proteomic analysis of human osteoarthritic chondrocytes reveals protein changes in stress and glycolysis. Proteomics. 8:495–507. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Nugent AE, Speicher DM, Gradisar I, McBurney DL, Baraga A, Doane KJ and Horton WE Jr: Advanced osteoarthritis in humans is associated with altered collagen VI expression and upregulation of ER-stress markers Grp78 and bag-1. J Histochem Cytochem. 57:923–931. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Intekhab-Alam NY, White OB, Getting SJ, Petsa A, Knight RA, Chowdrey HS, Townsend PA, Lawrence KM and Locke IC: Urocortin protects chondrocytes from NO-induced apoptosis: A future therapy for osteoarthritis? Cell Death Dis. 4:e7172013. View Article : Google Scholar : PubMed/NCBI

14 

Hardison WG and Grundy SM: Effect of ursodeoxycholate and its taurine conjugate on bile acid synthesis and cholesterol absorption. Gastroenterology. 87:130–135. 1984.PubMed/NCBI

15 

Ozcan U, Yilmaz E, Ozcan L, Furuhashi M, Vaillancourt E, Smith RO, Görgün CZ and Hotamisligil GS: Chemical chap-erones reduce ER stress and restore glucose homeostasis in a mouse model of type 2 diabetes. Science. 313:1137–1140. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Chen Y, Liu CP, Xu KF, Mao XD, Lu YB, Fang L, Yang JW and Liu C: Effect of taurine-conjugated ursodeoxycholic acid on endoplasmic reticulum stress and apoptosis induced by advanced glycation end products in cultured mouse podocytes. Am J Nephrol. 28:1014–1022. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Hochberg MC, Altman RD, Brandt KD, Clark BM, Dieppe PA, Griffin MR, Moskowitz RW and Schnitzer TJ; American College of Rheumatology: Guidelines for the medical management of osteoarthritis. Part II Osteoarthritis of the knee. Arthritis Rheum. 38:1541–1546. 1995. View Article : Google Scholar : PubMed/NCBI

18 

Outerbridge RE: The etiology of chondromalacia patellae. J Bone Joint Surg Br. 43-B:752–757. 1961.PubMed/NCBI

19 

Xu C, Bailly-Maitre B and Reed JC: Endoplasmic reticulum stress: Cell life and death decisions. J Clin Invest. 115:2656–2664. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Rao RV, Ellerby HM and Bredesen DE: Coupling endoplasmic reticulum stress to the cell death program. Cell Death Differ. 11:372–380. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Iwawaki T, Akai R, Kohno K and Miura M: A transgenic mouse model for monitoring endoplasmic reticulum stress. Nat Med. 10:98–102. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Oyadomari S and Mori M: Roles of CHOP/GADD153 in endoplasmic reticulum stress. Cell Death Differ. 11:381–389. 2004. View Article : Google Scholar

23 

Wang XZ, Lawson B, Brewer JW, Zinszner H, Sanjay A, Mi LJ, Boorstein R, Kreibich G, Hendershot LM and Ron D: Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153). Mol Cell Biol. 16:4273–4280. 1996.PubMed/NCBI

24 

Oliver BL, Cronin CG, Zhang-Benoit Y, Goldring MB and Tanzer ML: Divergent stress responses to IL-1beta, nitric oxide, and tunicamycin by chondrocytes. J Cell Physiol. 204:45–50. 2005. View Article : Google Scholar

25 

Matsuo M, Nishida K, Yoshida A, Murakami T and Inoue H: Expression of caspase-3 and -9 relevant to cartilage destruction and chondrocyte apoptosis in human osteoarthritic cartilage. Acta Med Okayama. 55:333–340. 2001.

26 

Pelletier JP, Jovanovic DV, Lascau-Coman V, Fernandes JC, Manning PT, Connor JR, Currie MG and Martel-Pelletier J: Selective inhibition of inducible nitric oxide synthase reduces progression of experimental osteoarthritis in vivo: Possible link with the reduction in chondrocyte apoptosis and caspase 3 level. Arthritis Rheum. 43:1290–1299. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Xie Q, Khaoustov VI, Chung CC, Sohn J, Krishnan B, Lewis DE and Yoffe B: Effect of tauroursodeoxycholic acid on endoplasmic reticulum stress-induced caspase-12 activation. Hepatology. 36:592–601. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Zhang J, Morris MW Jr, Dorsett-Martin WA, Drake LC and Anderson CD: Autophagy is involved in endoplasmic reticulum stress-induced cell death of rat hepatocytes. J Surg Res. 183:929–935. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Zhu Q, Zhong JJ, Jin JF, Yin XM and Miao H: Tauroursodeoxycholate, a chemical chaperone, prevents palmitate-induced apoptosis in pancreatic β-cells by reducing ER stress. Exp Clin Endocrinol Diabetes. 121:43–47. 2013.

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Copy and paste a formatted citation
Spandidos Publications style
Liu C, Cao Y, Yang X, Shan P and Liu H: Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in the chondrocytes of patients with osteoarthritis. Int J Mol Med 36: 1081-1087, 2015.
APA
Liu, C., Cao, Y., Yang, X., Shan, P., & Liu, H. (2015). Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in the chondrocytes of patients with osteoarthritis. International Journal of Molecular Medicine, 36, 1081-1087. https://doi.org/10.3892/ijmm.2015.2295
MLA
Liu, C., Cao, Y., Yang, X., Shan, P., Liu, H."Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in the chondrocytes of patients with osteoarthritis". International Journal of Molecular Medicine 36.4 (2015): 1081-1087.
Chicago
Liu, C., Cao, Y., Yang, X., Shan, P., Liu, H."Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in the chondrocytes of patients with osteoarthritis". International Journal of Molecular Medicine 36, no. 4 (2015): 1081-1087. https://doi.org/10.3892/ijmm.2015.2295
Copy and paste a formatted citation
x
Spandidos Publications style
Liu C, Cao Y, Yang X, Shan P and Liu H: Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in the chondrocytes of patients with osteoarthritis. Int J Mol Med 36: 1081-1087, 2015.
APA
Liu, C., Cao, Y., Yang, X., Shan, P., & Liu, H. (2015). Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in the chondrocytes of patients with osteoarthritis. International Journal of Molecular Medicine, 36, 1081-1087. https://doi.org/10.3892/ijmm.2015.2295
MLA
Liu, C., Cao, Y., Yang, X., Shan, P., Liu, H."Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in the chondrocytes of patients with osteoarthritis". International Journal of Molecular Medicine 36.4 (2015): 1081-1087.
Chicago
Liu, C., Cao, Y., Yang, X., Shan, P., Liu, H."Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in the chondrocytes of patients with osteoarthritis". International Journal of Molecular Medicine 36, no. 4 (2015): 1081-1087. https://doi.org/10.3892/ijmm.2015.2295
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