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International Journal of Molecular Medicine
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October-2015 Volume 36 Issue 4

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Article

miR‑485‑5p inhibits bladder cancer metastasis by targeting HMGA2

  • Authors:
    • Zhijun Chen
    • Qingwen Li
    • Sheng Wang
    • Jiajun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Urinary Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233003, P.R. China
  • Pages: 1136-1142
    |
    Published online on: August 3, 2015
       https://doi.org/10.3892/ijmm.2015.2302
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Abstract

MicroRNA (miRNA or miR)‑485 is a functional miRNA which has received much attention in recent years. However, little is known about the expression of miR‑485 or the role it plays in bladder cancer [namely in metastasis and epithelial‑mesenchymal transition (EMT)]. Thus, in the present study, we aimed to detect the expression of miR‑485 in human bladder cancer tissues and bladder cancer cell lines, and to examine the effects of miR‑485‑5p on bladder cancer cell metastasis and EMT. We found that the expression of miR‑485‑5p was downregulated in the human bladder cancer tissues and different bladder cancer cell lines compared with the normal tissues and cell lines, as demonstrated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). We enforced the expression of miR‑485‑5p in T24 cells and inhibited the expression of miR‑485‑5p in SW780 cells by transfection with miR‑485‑5p mimic or miR‑485‑5p inhibitor, respectively. The ectopic expression of miR‑485‑5p was shown to inhibit cell metastasis and EMT, whereas the inhibition of miR‑485‑5p expression promoted cell metastasis and EMT, as shown by transwell‑matrigel assay, cell adhesion assay and western blot analysis. Furthermore, a luciferase reporter assay revealed that high mobility group AT‑hook 2 (HMGA2) was a direct target of miR‑485‑5p and that the overexpression of HMGA2 reversed the effects of miR‑485‑5p on cell metastasis and EMT. In conclusion and to the very best of our knowledge, the present study, for the first time, identified miR‑485‑5p as a suppressive miRNA in human bladder cancer, and demonstrated that miR‑485‑5p inhibits cell metastasis and EMT at least partly through the suppression of HMGA2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Z, Li Q, Wang S and Zhang J: miR‑485‑5p inhibits bladder cancer metastasis by targeting HMGA2. Int J Mol Med 36: 1136-1142, 2015.
APA
Chen, Z., Li, Q., Wang, S., & Zhang, J. (2015). miR‑485‑5p inhibits bladder cancer metastasis by targeting HMGA2. International Journal of Molecular Medicine, 36, 1136-1142. https://doi.org/10.3892/ijmm.2015.2302
MLA
Chen, Z., Li, Q., Wang, S., Zhang, J."miR‑485‑5p inhibits bladder cancer metastasis by targeting HMGA2". International Journal of Molecular Medicine 36.4 (2015): 1136-1142.
Chicago
Chen, Z., Li, Q., Wang, S., Zhang, J."miR‑485‑5p inhibits bladder cancer metastasis by targeting HMGA2". International Journal of Molecular Medicine 36, no. 4 (2015): 1136-1142. https://doi.org/10.3892/ijmm.2015.2302
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Z, Li Q, Wang S and Zhang J: miR‑485‑5p inhibits bladder cancer metastasis by targeting HMGA2. Int J Mol Med 36: 1136-1142, 2015.
APA
Chen, Z., Li, Q., Wang, S., & Zhang, J. (2015). miR‑485‑5p inhibits bladder cancer metastasis by targeting HMGA2. International Journal of Molecular Medicine, 36, 1136-1142. https://doi.org/10.3892/ijmm.2015.2302
MLA
Chen, Z., Li, Q., Wang, S., Zhang, J."miR‑485‑5p inhibits bladder cancer metastasis by targeting HMGA2". International Journal of Molecular Medicine 36.4 (2015): 1136-1142.
Chicago
Chen, Z., Li, Q., Wang, S., Zhang, J."miR‑485‑5p inhibits bladder cancer metastasis by targeting HMGA2". International Journal of Molecular Medicine 36, no. 4 (2015): 1136-1142. https://doi.org/10.3892/ijmm.2015.2302
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