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International Journal of Molecular Medicine
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Article

The combined use of miRNAs and mRNAs as biomarkers for the diagnosis of papillary thyroid carcinoma

  • Authors:
    • Yinlong Zhao
    • Xiaodong Liu
    • Lili Zhong
    • Mengzi He
    • Silin Chen
    • Tiejun Wang
    • Shumei Ma
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Radiation Oncology, The Second Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 1097-1103
    |
    Published online on: August 5, 2015
       https://doi.org/10.3892/ijmm.2015.2305
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Abstract

Thyroid carcinoma (TC) is the most common malignancy of the endocrine system, and papillary thyroid carcinoma (PTC) accounts for the largest proportion of cases with TC. Although histology is considered the gold standard in the diagnosis of PTC, the sensitivity and specificity of this method is low. Therefore, developing novel diagnostic and prognostic biomarkers for PTC is essential. MicroRNAs (miRNAs or miRs) and their target RNAs play critical roles in tumorigenesis and tumor progression. Thus, the characteristic miRNA and mRNA expression profiles may function as diagnostic biomarkers for tumors, making it possible to predict the tumor stage and the prognosis of patients. In the present study, we detected miRNAs and mRNAs which can function as novel biomarkers for the diagnosis of PTC. The sensitivity of the diagnostic tests was evaluated by receiver operating characteristic curve analysis. Pearson's correlation analysis was used to determine the correlation between mRNAs and miRNAs, and cancer types. We found that the area under the curve (AUC) values of 8 miRNAs (miR-106a, miR-15a, miR-30a, miR-30b, miR-20a, miR-20b, miR-30d and miR-30e) and 8 mRNAs [axis inhibition protein 2 (AXIN2), integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) (ITGA3), tumor protein p53 inducible nuclear protein (TP53INP)1, TP53INP2, B-cell CLL/lymphoma 2 (BCL2), phosphatase and tensin homolog (PTEN), FOS and K(lysine) acetyltransferase 2B (KAT2B)] were >0.90. The combination of miR-15a and AXIN2 significantly improved the diagnostic accuracy. Therefore, our data indicate that the differential expression of miRNAs combined with that of their target mRNAs may serve as a powerful biomarker for distinguishing PTC from benign tissues.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Liu X, Zhong L, He M, Chen S, Wang T and Ma S: The combined use of miRNAs and mRNAs as biomarkers for the diagnosis of papillary thyroid carcinoma. Int J Mol Med 36: 1097-1103, 2015.
APA
Zhao, Y., Liu, X., Zhong, L., He, M., Chen, S., Wang, T., & Ma, S. (2015). The combined use of miRNAs and mRNAs as biomarkers for the diagnosis of papillary thyroid carcinoma. International Journal of Molecular Medicine, 36, 1097-1103. https://doi.org/10.3892/ijmm.2015.2305
MLA
Zhao, Y., Liu, X., Zhong, L., He, M., Chen, S., Wang, T., Ma, S."The combined use of miRNAs and mRNAs as biomarkers for the diagnosis of papillary thyroid carcinoma". International Journal of Molecular Medicine 36.4 (2015): 1097-1103.
Chicago
Zhao, Y., Liu, X., Zhong, L., He, M., Chen, S., Wang, T., Ma, S."The combined use of miRNAs and mRNAs as biomarkers for the diagnosis of papillary thyroid carcinoma". International Journal of Molecular Medicine 36, no. 4 (2015): 1097-1103. https://doi.org/10.3892/ijmm.2015.2305
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Liu X, Zhong L, He M, Chen S, Wang T and Ma S: The combined use of miRNAs and mRNAs as biomarkers for the diagnosis of papillary thyroid carcinoma. Int J Mol Med 36: 1097-1103, 2015.
APA
Zhao, Y., Liu, X., Zhong, L., He, M., Chen, S., Wang, T., & Ma, S. (2015). The combined use of miRNAs and mRNAs as biomarkers for the diagnosis of papillary thyroid carcinoma. International Journal of Molecular Medicine, 36, 1097-1103. https://doi.org/10.3892/ijmm.2015.2305
MLA
Zhao, Y., Liu, X., Zhong, L., He, M., Chen, S., Wang, T., Ma, S."The combined use of miRNAs and mRNAs as biomarkers for the diagnosis of papillary thyroid carcinoma". International Journal of Molecular Medicine 36.4 (2015): 1097-1103.
Chicago
Zhao, Y., Liu, X., Zhong, L., He, M., Chen, S., Wang, T., Ma, S."The combined use of miRNAs and mRNAs as biomarkers for the diagnosis of papillary thyroid carcinoma". International Journal of Molecular Medicine 36, no. 4 (2015): 1097-1103. https://doi.org/10.3892/ijmm.2015.2305
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